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Activated fluorescent latex microspheres for ferritin fluorescent immunochromatographic detection card

A technology of fluorescent latex microspheres and fluorescent immunochromatography, applied in analytical materials, luminescent materials, biological testing, etc., can solve problems such as reduced detection sensitivity, uneven dispersion, and chromatographic failure, and achieve stable and accurate detection results. Accurate, easy-to-operate results

Active Publication Date: 2018-01-05
TIANJIN SAVANT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: after drying, the fluorescent latex microspheres of the ferritin (Fer) fluorescence immunochromatography detection card are easy to agglomerate and disperse unevenly, resulting in chromatographic failure or a decrease in detection sensitivity

Method used

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  • Activated fluorescent latex microspheres for ferritin fluorescent immunochromatographic detection card
  • Activated fluorescent latex microspheres for ferritin fluorescent immunochromatographic detection card
  • Activated fluorescent latex microspheres for ferritin fluorescent immunochromatographic detection card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1) Take the surfactant (2mg sodium laurylalanine, 3mg polyethylene glycol monolaurate and 5mg sodium stearate) first dissolved in 0.5M carbonic acid buffer solution of pH9.6 (H value to 9.6 ), then add in 0.1ml of DMF, 0.1ml of DMF, add 1mg of N,N'-dicyclohexylcarbodiimide (DCC) and 0.6mg of N-hydroxysuccinimide (NHS) and stir at room temperature 3 hours.

[0047] 2) Adjust the pH value of 1ml of fluorescent latex microsphere solution containing 1% solids on the amino surface to 9.6 with 0.5M carbonic acid buffer solution of pH 9.6, then add it to step 1), and continue to stir and react for 3 hours. Acquired activated latex microspheres.

[0048] 3) Use a high-speed centrifuge to centrifuge the fluorescent latex microsphere reaction solution at high speed, remove the supernatant of the reaction solution, and redissolve the fluorescent latex microspheres with 1mL microsphere buffer to form a marker working solution.

[0049] Microsphere phosphate buffer preparation: 0....

Embodiment 2

[0051] The operation method of this example is similar to that of Example 1, except that the surfactant in step 1) is: 0.5mg sodium laurylalanine, 2.5mg polyethylene glycol monolaurate and 5mg hard sodium fatty acid.

Embodiment 3

[0053] The operation method of this example is similar to that of Example 1, except that the surfactant in step 1) is: 5 mg sodium laurylalanine, 0.5 mg polyethylene glycol monolaurate and 4.5 mg hard sodium fatty acid.

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Abstract

The invention discloses an activated fluorescent latex microsphere used for a ferritin fluorescence immunoassay chromatography detection card, and application of the activated fluorescent latex microsphere. A preparation method of the activated fluorescent latex microsphere used for the ferritin fluorescence immunoassay chromatography detection card comprises the following steps: 1) adding a surfactant into buffered solution of which the pH is 8-10, and adding dimethylformamide, N,N'-dicyclohexylcarbodiimide and N-hydroxy succinimide; 2) taking dispersion liquid of the fluorescent latex microsphere on an amino surface, regulating the pH to 8-10 by the buffered solution, adding into a mixture obtained in 1), carrying out stirring reaction, and removing supernate by centrifugation after reaction is finished, so as to obtain a surface-activated fluorescent latex microsphere, wherein the surfactant is a mixture of an amino acid-based surfactant, polyethylene glycol monolaurate and stearate at the mass ratio of (1 to 10):(1 to 5): (1 to 10).

Description

technical field [0001] The invention belongs to the technical field of fluorescence immunochromatography, and in particular relates to an activated fluorescent latex microsphere for a ferritin fluorescence immunochromatography detection card and its preparation and application. Background technique [0002] Fluorescence immunochromatography detection card (reagent card) is widely used in the field of rapid detection because of its fast and convenient characteristics. The analyte antibody is labeled with fluorescent latex microspheres, and the latex microspheres have surface electron repulsion and van der Waals forces in the liquid phase to keep the latex particles in the liquid phase evenly distributed. However, after spraying on the glass cellulose film and drying, as the solution is evaporated to dryness, the surface tension pushes the latex particles infinitely closer together, resulting in the disappearance of the intermolecular repulsion, and the increase of the van der...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/543G01N33/533C09K11/06C09K11/02
CPCC09K11/02C09K11/06C09K2211/1425G01N33/533G01N33/54313G01N33/558G01N33/577G01N33/6893G01N2333/47G01N2800/22
Inventor 林斯
Owner TIANJIN SAVANT BIOTECH CO LTD