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Application of berberine and bacillus licheniformis composition in preparation of drug for preventing or treating diseases caused rotavirus infection

A technology of Bacillus licheniformis and rotavirus, applied in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of easy drug resistance and inability to take long-term medication

Active Publication Date: 2017-02-22
NORTHEAST PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, anti-rotavirus drugs such as ribavirin are often used in clinical anti-rotavirus treatment, but they are prone to drug resistance and cannot be used for a long time

Method used

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  • Application of berberine and bacillus licheniformis composition in preparation of drug for preventing or treating diseases caused rotavirus infection
  • Application of berberine and bacillus licheniformis composition in preparation of drug for preventing or treating diseases caused rotavirus infection
  • Application of berberine and bacillus licheniformis composition in preparation of drug for preventing or treating diseases caused rotavirus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] This example proves that a certain dose of Bacillus licheniformis 63516 strain original bacteria powder and berberine hydrochloride can synergistically prevent and treat rotavirus Wa strain infection, and its effect is significantly better than that of Bacillus licheniformis or berberine hydrochloride alone .

[0013] 1. Cell strains and virus strains:

[0014] Rhesus monkey embryonic kidney cells (MA-104) (ATTC#CRL-2378.1) were purchased from China Center for Type Culture Collection. Human rotavirus strain Wa was purchased from China Microorganism Strain Network, number CVCCAV54.

[0015] 2. MA-104 cell culture:

[0016] MA-104 was cultured with minimal essential medium (MEM) containing 10% calf serum and 1% non-essential amino acids at a cell density of 5×10 5 / ml inoculated in culture flasks, placed at 37°C with 5% CO 2 After static culture in the incubator for 48 hours, after growing into a good dense monolayer of cells, it was used for subsequent experiments. ...

Embodiment 2

[0046] Take 20g of the original bacteria powder, 25g of berberine hydrochloride, 30g of starch, 10g of lactose and 2.5g of carboxymethyl starch sodium and mix thoroughly, add an appropriate amount of water to make a soft material, and pass the soft material through a 40-mesh sieve to obtain wet granules. Dry the wet granules by box drying method, that is to say.

Embodiment 3

[0048] Take 15g of the original bacteria powder, 75g of berberine hydrochloride, 60g of microcrystalline cellulose, 35g of lactose and 7g of low-substituted hydroxypropyl cellulose, mix well, add an appropriate amount of water to make a soft material, pass the soft material through a 40-mesh sieve, and make To obtain wet granules, dry the wet granules by box drying method.

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Abstract

The invention provides application of a berberine and bacillus licheniformis composition in preparation of a drug for preventing or treating diseases caused rotavirus infection and relates to the field of drug compositions and novel application thereof. The berberine and bacillus licheniformis composition is used for preventing or treating enteritis, diarrhea and other diseases caused by rotavirus infection and has the advantages of being efficient, low in toxicity and the like.

Description

technical field [0001] The invention belongs to the field of new drug combinations and new applications thereof, in particular to the application of a combination of berberine and bacillus licheniformis in the preparation of drugs for preventing or treating rotavirus infection. Background technique [0002] Rotavirus (Rotavirus, RV) belongs to the Reoviridae family, and is the most important pathogen causing severe diarrhea in infants and young children. Children infected with RV have high morbidity and mortality. About 453,000 people died of RV infection. RV infection not only causes enteritis and diarrhea, but also causes myocardial and liver damage in younger children. As the main incidence of RV is children, hospitalization costs and family disease burden are relatively high. Moreover, epidemiological studies have shown that economic level Improvement of health conditions and sanitation did not reduce the incidence of RV infectious diarrhea. Although many rotavirus vac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/742A61P31/14A61K31/4375
CPCA61K31/4375A61K35/742A61K2300/00
Inventor 苏显英雷健彭勃于雯郭凤尧吴楠韩微魏斌戴畅
Owner NORTHEAST PHARMA GRP
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