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Anti-ultraviolet high-toxicity meterhizium anisopliae mutant strain MaUV-1 and application thereof

An anti-ultraviolet technology of Metarhizium anisopliae, applied in the application, fungi, mutant preparation and other directions, can solve the problems of poor ultraviolet tolerance, poor control effect, weak virulence, etc., and achieves good anti-ultraviolet ability, The effect of improving the control effect and strong infestation and insecticidal effect

Active Publication Date: 2017-02-22
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, my country's existing Metarhizium anisopliae ( Metarhizium anisopliae ) The virulence of the strain is not strong, and the tolerance to ultraviolet rays is poor, so the control effect is poor

Method used

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  • Anti-ultraviolet high-toxicity meterhizium anisopliae mutant strain MaUV-1 and application thereof
  • Anti-ultraviolet high-toxicity meterhizium anisopliae mutant strain MaUV-1 and application thereof
  • Anti-ultraviolet high-toxicity meterhizium anisopliae mutant strain MaUV-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Mutagenesis and isolation and identification of wild strains

[0033] 1. Materials

[0034] The mutant strain of Metarhizium anisopliae is derived from the wild strain, and the wild-type Metarhizium anisopliae strain is collected from scarabs that are naturally infected in Tibet ( Phyllopertha horticola L.) Larval carcasses.

[0035] Czapek-Dox: NaNO 3 2 g, K 2 HPO 4 1 g, KCl 0.5 g, MgSO 4 0.5 g, FeSO 4 0.01 g, 30 g sucrose, 20 g agar, add water to make a total volume of 1000 ml and bring to a boil. Afterwards, it was sterilized by autoclaving (121°C, 30min).

[0036] Sterile operating conditions: All utensils and utensils must be sterilized by autoclaving (121°C, 30min), and operations such as inoculation are performed in an ultra-clean workbench.

[0037] Culture conditions: Culture in a 25°C light (14L: 10D) incubator. After the colony is formed, transfer it to a PDA slope, and then transfer it to a 4°C refrigerator for storage.

[0038] 2. Mu...

Embodiment 2

[0083] Example 2 Determination of the pathogenicity of the mutant strain of Metarhizium anisopliae (MaUV-1) to Plutella xylostella

[0084] 1. Bioassay is one of the effective means to detect the lethality and lethality rate of entomogenic fungi to target pests, and can provide an important reference for comprehensive evaluation of the biological control potential of the fungus. The invention measures the pathogenicity of the metarhizium anisopliae mutant strain MaUV-1 to the diamondback moth, so as to screen out the optimal concentration for control.

[0085] Insects and host plants to be tested: Plutella xylostella adults raised on kale in the net room were inoculated onto kale seedlings without insects, and after 12 hours of oviposition, the adults were removed, and the kale seedlings were placed in a 25±0.5℃ In the light incubator, wait until the diamondback moth develops to the 2nd instar larvae. kale Brassica campestris L., purchased from Vegetable Research Institute ...

Embodiment 3

[0097] Example 3 Determination of the pathogenicity of the mutant strain of Metarhizium anisopliae MaUV-1 to the red fire ants

[0098] 1. Insects to be tested: red fire ants, collected from the lawn of Guangzhou University City, were collected and kept indoors for a period of time. After the population stabilized, healthy large worker ants of the same size were selected for the test.

[0099] (1) Treatment of the tested strains

[0100] Take the mutant strain of Metarhizium anisopliae (MaUV-1) and the wild strain (WT) and inoculate them on the Czapek plate, and culture them in an incubator (14L:10D) at 25 ± 0.5°C for 14 days, and add 0.3% Tween after sporulation -80 sterile water to collect spores, disperse the spore suspension evenly on a magnetic stirrer, filter with medical gauze to remove impurities, obtain the spore suspension, count it with a hemocytometer, and make it into 0, 1×10 3 , 1×10 4 , 1×10 5 , 1×10 6 , 1×10 7 A total of 6 concentration gradients of spores...

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Abstract

The invention discloses an anti-ultraviolet high-toxicity meterhizium anisopliae mutant strain MaUV-1 and application thereof. The meterhizium anisopliae mutant strain MaUV-1 is obtained due to ultraviolet mutation screening of wild metarhizium anisopliae. The strain is preserved in the China Center for Type Culture Collection in May 6th, 2016, and the preservation number is CCTCC NO:M 2016250. Through infection biological study and indoor biological test, the meterhizium anisopliae mutant strain MaUV-1 has a strong infection insect killing effect on pests like insecticide-resistant plutella xylostella and solenopsis invicta, and is high in anti-ultraviolet capacity. Compared with a wild strain, toxicity of the mutant strain which is stable in inheritance to plutella xylostella is improved by 75%, toxicity to solenopsis invicta is improved by 39-78%, and the anti-ultraviolet capacity is improved by 70-189%.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a mutagenic strain MaUV-1 of an ultraviolet-resistant and highly virulent Metarhizium anisopliae and its application. Background technique [0002] Pests such as diamondback moth and fire ants can harm a variety of crops and cause huge economic losses. Long-term use of chemical pesticides has resulted in the emergence of pest resistance, making control more difficult. [0003] In the control of resistant pests such as diamondback moth and red fire ants, traditional chemical control methods using a large number of chemical pesticides are mostly used. However, pests such as diamondback moth and red imported fire ants have developed a high level of resistance to most of the commonly used chemical pesticides , and the RIFA lives in the soil, and it is difficult to control it with chemical pesticides. [0004] Biological control is an ideal and environmentally friendly me...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/01C07K14/37A01N63/04A01P7/04C12R1/645
CPCA01N63/30C07K14/37C12N1/145C12R2001/645
Inventor 黄振
Owner SOUTH CHINA AGRI UNIV
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