Citric acid bacillus Y3 with function of degrading brominated flame retardants and application thereof
A brominated flame retardant, Citrobacter technology, applied in bacteria, microbial-based methods, water/sludge/sewage treatment, etc.
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Embodiment 1
[0036] Example 1 Isolation and Identification of Citrobacter Y3
[0037] 1. Enrichment and isolation of strains
[0038] Continuous-flow anaerobic reaction with hexabromocyclododecane as a contaminant grown for 2 years from the laboratory
[0039] Take out 5ml of the mud-water mixture from the instrument and culture it in a standard serum bottle. At the same time, add an inorganic salt culture solution containing 5mg / L hexabromocyclododecane (see Table 1 for the composition of the culture solution), and shake it at an anaerobic temperature of 30°C and a speed of 120 rap. After one week of enrichment and acclimatization in the bed, transfer the inorganic salt medium containing 8mg / L hexabromocyclododecane with 10% inoculation amount and then acclimatize for one week, and gradually increase the concentration of hexabromocyclododecane for acclimatization by analogy, six The concentration of bromocyclododecane is 10, 15, 20mg / L respectively. After acclimatization and enrichment...
Embodiment 2
[0093] Example 2 Degradation test of Citrobacter Y3 to hexabromocyclododecane under anaerobic co-metabolism conditions
[0094] 1. For the isolated and purified Citrobacter Y3 under anaerobic conditions and the serum bottle experiment of the degradation of hexabromocyclododecane without adding co-metabolism substrates, samples were taken every 2 days until the eighth day, and the detection of hexabromocyclododecane The degradation rate of dioxane and the growth of strain OD 600nm .
[0095] 2. After the experiment, the inventors found that under the condition of using hexabromocyclododecane as a single carbon source, the growth of the bacteria was very slow within eight days, and the degradation efficiency of hexabromocyclododecane was only about 40%, which shows that , Hexabromocyclododecane is a refractory persistent organic compound, and the strain may lack the corresponding enzyme in the body, so it is difficult to use hexabromocyclododecane as an energy source to suppo...
Embodiment 3
[0098] Example 3 Effects of Different Concentrations of Co-Metabolite Substrates on the Degradation of Hexabromocyclododecane by Citrobacter Y3
[0099] The inventors also explored the effects of different concentrations and different types of co-metabolite substrates on the degradation of hexabromocyclododecane by strain Y3, respectively setting up gradient experiments with different concentrations of glucose, maltose, sucrose, starch and sodium citrate, and comparing their effects on the hexabromocyclododecane. The impact of bromocyclododecane degradation efficiency, determine the optimum range or optimum ratio under optimum degradation conditions.
[0100] Firstly, five different concentrations (10, 100, 500, 1000, 2000 mg / L) were set according to the different ratios of the co-metabolism substrate and the pollutant HBCD to explore the optimum between the co-metabolism substrate and the pollutant substrate. relation. As we all know, the ratio of growth substrate and non-grow...
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