Quick osseous tissue RNA extraction kit

Abstract

The invention discloses a quick osseous tissue RNA extraction kit. The kit comprises at least a genomic DNA removal column, at least one RNA absorptive column, lysis buffer used cooperatively with the genomic DNA removal column and elution buffer used cooperatively with the RNA absorptive column. The kit has the advantages that the exclusive phenol / chloroform-free lysis buffer is used and a plurality of ingredients are added to remove proteoglycan. A particular buffer system and a bimodal pore silica gel purification system are utilized to solve the problem of remaining DNA, thus osseous tissue RNA with high purity and good integrity can be obtained. The particular genomic DNA removal column can effectively remove remaining genomic DNA (gDNA), thus the extracted RNA can be directly used in reverse transcription PCR and real-time PCR without DNase digestion. Extraction of single samples can be completed in 35 minutes generally, and RNA degradation due to long-time extraction, RNase contamination and other causes is avoided.

Description

technical field [0001] The invention relates to RNA extraction, and relates to the field of nucleic acid purification, in particular to a rapid extraction kit for bone tissue RNA. Background technique [0002] The research of molecular biology mainly focuses on nucleic acid and protein. Most molecular biology research needs to obtain purified nucleic acid first. Therefore, nucleic acid purification is one of the cornerstones of molecular biology. The global market is very large, and countries all over the world are doing Continuously improve the extraction and purification methods to achieve faster and purer nucleic acid. Nucleic acid purification is generally divided into two categories: RNA extraction and purification and DNA extraction and purification. [0003] There are mainly two mainstream RNA extraction solutions in the world. One is the guanidine isothiocyanate / acid phenol / chloroform one-step extraction of total RNA (commonly known as the TRIzol method) invented by...

AI Technical Summary

Problems solved by technology

The disadvantage is that the silica gel column will adsorb RNA / DNA at the same time, so it is easy to cause DNA residue, resulting in low purity

[0004] The bone tissue is hard, the bone tissue is hard, the bone cell density is low, and the peripheral matrix contains a large amount of mucin (proteoglycan) and RNA is difficult to separate, and high-quality extraction cannot be performed by the traditional Trizol method

In the process of extracting bone tissue RNA, the RNA is often degraded due to long extraction time, RNase contamination and other reasons, making subsequent experiments impossible

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