Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of lactobacillus pentosus SS6 to producing gamma-aminobutyric acid

A technology of Lactobacillus pentosus and aminobutyric acid, applied in the field of microorganisms, can solve the problems of slow growth of fungi, high cost, serious environmental pollution, etc., and achieve the effects of promoting growth hormone secretion, lowering cholesterol, and having wide application prospects.

Active Publication Date: 2017-02-22
武汉蜜欢健康产业发展有限公司
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the early days, enrichment production of GABA was mainly based on chemical synthesis, plant enrichment and fermentation by Escherichia coli, but there were problems such as poor safety, high cost, serious environmental pollution, low enrichment content and food safety. , so it is of potential significance and value to search for available food-grade safe strains in the enrichment of GABA by microbial methods
[0004] Most of the gamma-aminobutyric acid-producing microorganisms isolated by the prior art are fungi, which grow slowly and cannot meet production needs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of lactobacillus pentosus SS6 to producing gamma-aminobutyric acid
  • Application of lactobacillus pentosus SS6 to producing gamma-aminobutyric acid
  • Application of lactobacillus pentosus SS6 to producing gamma-aminobutyric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Isolation, screening and identification of bacterial strains

[0025] Isolation of strains: Select mature mulberry fruits from healthy growing mulberry trees, rinse them with sterile water for 3 times in a sterile ultra-clean bench, then dry them with sterile filter paper, cut them into small pieces of 1 cm, put them in a mortar and grind them to get the juice, and absorb 100 μL The fruit juice was cultured in the MRS liquid medium for 2 days in a constant temperature incubator at 30°C. When the liquid medium became turbid, the bacterial liquid was diluted by the gradient dilution method, and the dilution was 10. -5 and 10 -6 Put 100 μL of the bacterial cell dilution solution in the lactic acid bacteria isolation medium, and incubate in a 30°C constant temperature incubator for 2 to 3 days. After colonies grow in the lactic acid bacteria isolation medium (round milky white or yellow colonies appear), the surrounding medium turns yellow. It was determined to b...

Embodiment 2

[0035] The cultivation of embodiment 2 bacterial strains

[0036] 1. Activation of Lactobacillus pentosus SS6: Aseptically operate in an ultra-clean workbench, pick Lactobacillus pentosus SS6 with an inoculation loop, streak on a plate medium (MRS medium) with an inoculation loop, and culture at 30°C Cultured in the box for 2 days.

[0037] 2. Preparation of fermentation samples of Lactobacillus pentosus SS6: Inoculate the isolated Lactobacillus pentosus SS6 into 50 mL of fermentation medium TYG, culture it in a 30°C incubator for 2 days, place the fermentation mixture in a boiling water bath for 10 minutes, and heat at 10000 r / h Centrifuge for 10 min, and take the upper liquid to obtain the fermentation broth.

[0038] 3. Drawing of γ-aminobutyric acid standard curve: Take 1.0mL distilled water and standard sample solutions of γ-aminobutyric acid with concentrations of 1mg / mL, 2mg / mL, 3mg / mL, 4mg / mL and 5mg / mL respectively, Add 120μL 1mol / L LaCl 3 Solution, mix and shake f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of lactobacillus pentosus SS6 to producing gamma-aminobutyric acid. The lactobacillus pentosus SS6 is preserved at the Microbiology Strain Preservation Center in Guangdong province on the preservation date of October 13th, 2016, the preservation address is the Institute of Microbiology in Guangdong province, and a preservation number of the lactobacillus pentosus SS6 is GDMCC No.60086. The application has the advantages that the content of the GABA (gamma-aminobutyric acid) fermented and produced by the aid of the lactobacillus pentosus can reach 3.09 mg / mL, the GABA has diversified beneficial healthcare functions of lowering blood pressures and cholesterol, promoting growth hormone secretion and the like, the basis can be provided for developing novel healthcare foods with abundant gamma-aminobutyric acid, and the lactobacillus pentosus SS6 has a broad application prospect when applied to developing the foods and healthcare products with the abundant GABA.

Description

technical field [0001] The invention relates to the technical field of microbes, more specifically, the application of Lactobacillus pentosus SS6 in producing gamma-aminobutyric acid. Background technique [0002] Gamma-aminobutyric acid (GABA) has many important physiological functions, such as anti-anxiety, lowering blood pressure, lowering blood sugar, improving sleep, repairing skin (inhibiting inflammation, stimulating epidermal cell regeneration), regulating hormones, etc. At this stage, with the continuous research and development of GABA's physiological and pharmacological functions, it has been used as a new type of functional factor in food and pharmaceutical industries. [0003] In the early days, enrichment production of GABA was mainly based on chemical synthesis, plant enrichment and fermentation by Escherichia coli, but there were problems such as poor safety, high cost, serious environmental pollution, low enrichment content and food safety. , so finding ava...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P13/00C12N1/20C12R1/225
CPCC12P13/005C12N1/205C12R2001/225
Inventor 陈芳艳吴珊林健荣钟杨生
Owner 武汉蜜欢健康产业发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com