Droplet PCR (Polymerase Chain Reaction) kit for detecting mutation of human KRAS gene

Abstract

The application relates to the field of the detection of gene mutation, and particularly speaking, relates to a droplet PCR (Polymerase Chain Reaction) kit for detecting the mutation of a human KRAS gene. The kit comprises a nucleic acid amplification reagent and a reference substance, wherein KRASddPCR reaction liquid contains primers and a probe which are used for detecting the mutation of No. 12 and No. 13 codon. A digital PCR technique is adopted for the kit provided by the application; quantitative detection is carried out on the mutation states of the No. 12 and No. 13 codon of a K-ras gene of a human being; the droplet PCR kit can be used for detecting the mutation states of the K-ras genes in free DNA (Deoxyribonucleic Acid) in paraffin-embedded pathological section tissues or the peripheral blood of patients suffered from colorectal cancer, lung cancer and the like; a reference is provided for a clinical doctor to select a tumor-targeted medicine for treatment and monitoring; the droplet PCR kit has absolute quantification, high specificity, accuracy and high sensitivity.

Description

technical field [0001] The application relates to the field of gene mutation detection, in particular to a microdroplet PCR kit for detecting human KRAS gene mutation. Background technique [0002] In tumor treatment, traditional chemotherapy mainly targets the DNA of cells, which often lacks specificity. While killing tumor cells, it also "wrongly kills" many normal cells. Targeted therapy is completely different from previous chemotherapy. There are many mechanisms involved in the pathogenesis of tumors, and these mechanisms are often unique to tumor cells. Targeted therapy is aimed at a specific signal transduction pathway or a certain pathogenesis in the pathogenesis of tumors, using monoclonal antibodies and small molecular substances to interrupt it, so as to achieve the purpose of treating tumors, but has a slight effect on normal cells. Targeted drugs intervene in the gene signaling pathways that control the growth and proliferation of tumor cells by binding to the...

AI Technical Summary

Problems solved by technology

At present, the detection of K-ras gene mutation status generally uses tissue, but in clinical application, tissue samples are not easy to obtain, for example, for patients in the advanced stage, elderly patients, patients with important organ dysfunction, patients with a large number of thoracic and abdominal cavity Patients with effusions cannot tolerate tissue biopsy puncture sampling operations. In addition, drug resistance is the ultimate doom for most patients

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