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Hemofiltration sample pad and preparation method thereof

A sample pad and blood filtration technology, applied in the field of medical testing, can solve problems such as affecting the speed of testing samples and testing results, increasing the thickness of reagent strips, and unfavorable production operations, so as to improve solubility, improve uniformity, and promote enterprises. effect of development

Active Publication Date: 2017-02-22
HANGZHOU BIOTEST BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are certain defects in the existing blood filter membrane, such as: 1. When the test reagent strip is used to detect the whole blood sample, it often affects the running speed and test results of the test sample, and there are traces of blood residue, white lines, etc.
2. The existing technology has different requirements for the length of the membrane according to the different packaging forms of the final product, and targeted debugging is required; 3. When using the blood filtration membrane, it is necessary to add a layer of sealing tape to increase the thickness of the reagent strip itself. It is not conducive to production operations; 4. In the process of sticking the blood membrane, it is necessary to pay attention to the front and back operations. In mass production, operation errors are prone to occur

Method used

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  • Hemofiltration sample pad and preparation method thereof
  • Hemofiltration sample pad and preparation method thereof
  • Hemofiltration sample pad and preparation method thereof

Examples

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preparation example Construction

[0037] The preparation steps of the sample pad are as follows:

[0038] 1. According to the performance of different products, the raw materials of each component are formulated into a solution according to the corresponding proportion and order, and the pH value of the prepared solution is adjusted to form the best buffer system suitable for the antigen-antibody reaction;

[0039] 2. Calculate the required amount of solution according to the corresponding glass fiber liquid absorption coefficient, and evenly treat the prepared treatment solution on the glass fiber;

[0040] 3. Dry the glass fibers treated with the treatment solution in an oven or drying room at 37°C for 8-24 hours.

Embodiment 1

[0041] Embodiment 1: the blood filter sample pad processing of human immunodeficiency virus (HIV 1 / 2) antibody detection reagent (latex method) product

[0042] The preparation steps of the filtered blood sample pad are as follows:

[0043] 1) Prepare the required amount of 0.9 purified water for the solution, add 0.04-0.06M Tris, 0.15-0.25mM Casein, 0.15-0.35mM PVP in sequence, stir the previous component to completely dissolve and then add the next component, Stir until completely dissolved;

[0044] 2) Add in order to the solution prepared in step 1): 0.01-0.015M sodium carbonate (hereinafter abbreviated as: Na 2 CO 3 ), 0.6-1.1% surfactant of the required treatment solution, 3-5% of the mouse-derived anti-RBC of the required treatment solution, the previous component is stirred and completely dissolved, then the next component is added, and stirred until completely dissolved, the solution clarify;

[0045] 3) adjust the pH value to 8.0±0.1 with hydrochloric acid or sod...

Embodiment 2

[0048] Embodiment 2: Hepatitis B virus surface antigen detection reagent (colloidal gold method) product sample pad processing

[0049] The preparation steps of the sample pad are as follows:

[0050] 1) Take 0.7% purified water required for the treatment solution, add 0.04-0.06M Tris, 0.1-0.3mM PVP, 0.02-0.1% surfactant, 0.15-0.25mM PVP to the purified water in order Casein, 0.02-0.04M ethylenediaminetetraacetic acid, 8-12% protein stabilizer in the required treatment solution, 2-5% sheep-derived anti-RBC in the required treatment solution, after the former component is stirred and completely dissolved Add the next component and stir until completely dissolved and the solution is clear;

[0051] 2) Adjust the pH value of the solution to 8.0±0.1 with hydrochloric acid or sodium hydroxide solution;

[0052] 3) Dilute the solution to the required solution volume with purified water, and retest the pH value at 8.0±0.1;

[0053] 4) Evenly treat the prepared treatment solution on ...

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Abstract

The invention relates to a hemofiltration sample pad. According to a preparation mode, a certain proportion of human erythrocyte antibodies, trihydroxymethyl aminomethane, casein, polyvinylpyrrolidone, surfactants and the like are prepared into a treatment solution according to different product properties in accordance with different sequences; the prepared solution is subjected to pH regulation so as to form a final treatment solution; the prepared treatment solution is uniformly processed on glass fiber and is put into a 37-DEG C drying box to be dried for 8 to 24 hours. The hemofiltration sample pad prepared by the method has the advantages that the whole blood sample is separated under the condition of not influencing the sample running board; a fast diagnosis reagent subjected to sample feeding has clean background without phenomena such as blood residue traces, flooding, line whitening or rail lines.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a whole blood separation membrane for whole blood testing and a preparation method thereof. [0002] technical background [0003] At present, the main material used in the blood filtration sample pad is whole blood separation membrane, also known as blood filtration membrane or plasma separation membrane, which can quickly separate the plasma in whole blood without hemolysis, so that the end-use customers of the product do not need to centrifuge the blood into Serum, which further saves detection time and increases efficiency, can directly use peripheral blood or fresh blood. Blood filtration membrane separation of whole blood intercepts red blood cells and white blood cells in whole blood through physical separation. [0004] The whole blood separation membrane is added to the test reagent strip, no additional device is required, and it is convenient to carry and use; the separat...

Claims

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Application Information

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IPC IPC(8): G01N1/34
CPCG01N1/34
Inventor 侯鲁娜叶春生
Owner HANGZHOU BIOTEST BIOTECH CO LTD
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