A method for detecting alginic acid content in brown algae and its products
A detection method and technology of alginic acid, applied in the field of analytical chemistry
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Embodiment 1
[0080] 1. Prepare the test solution:
[0081] (1) Hydrolysis of brown algae:
[0082] Accurately weigh 1.25g of brown algae (accurate to 0.0001g), place it in a 25ml volumetric flask, add water to fully dissolve it, make the volume constant, then accurately pipette 1ml into a 20ml hydrolysis flask (liquid directly and accurately divide 1ml), add 1.0ml Concentrated sulfuric acid with a concentration of 98% is then hydrolyzed in a water bath at 82°C for 30 minutes, taken out and cooled to room temperature, diluted with 5ml of water, and then placed in a water bath at 82°C for 30 minutes;
[0083] (2) Derivation of test products:
[0084] Take out step (1) The hydrolyzed product is placed in an ice-water bath to cool, and then neutralized with 8mol / L sodium hydroxide solution, adjust the pH to neutral, then transfer to a 25ml volumetric flask to constant volume, take 1ml solution in a 10ml volumetric flask Add 1ml of 0.3mol / L sodium hydroxide solution and 1ml of 0.5mol / L PMP (1-phenyl-3...
Embodiment 2
[0128] 1. Prepare the test solution:
[0129] (1) Hydrolysis of brown algae:
[0130] Accurately weigh 1.25g of brown algae (accurate to 0.0001g), place it in a 25ml volumetric flask, add water to fully dissolve it, make the volume constant, then accurately pipette 1ml into a 20ml hydrolysis flask (precisely aliquot 1ml of liquid), add 0.5ml Concentrated sulfuric acid with a concentration of 90%, then hydrolyzed in a 75°C water bath for 60 minutes, taken out and cooled to room temperature, diluted with 5ml of water, and then placed in a 75°C water bath to continue hydrolysis for 60 minutes;
[0131] (2) Derivation of test products:
[0132] Take out step (1) The hydrolyzed product is placed in an ice-water bath to cool, and then neutralized with 8mol / L sodium hydroxide solution, adjust the pH to neutral, then transfer to a 25ml volumetric flask to constant volume, take 1ml solution in a 10ml volumetric flask Add 0.5ml of 0.2mol / L sodium hydroxide solution and 0.5ml of 1mol / L PMP solu...
Embodiment 3
[0151] 1. Prepare the test solution:
[0152] (1) Hydrolysis of brown algae:
[0153] Accurately weigh 1.25g of brown algae (accurate to 0.0001g), place it in a 25ml volumetric flask, add water to fully dissolve it, make the volume constant, then accurately pipette 1ml into a 20ml hydrolysis flask (liquid directly and accurately divide 1ml), add 1.0ml Concentrated sulfuric acid with a concentration of 95% is then hydrolyzed in a water bath at 90°C for 90 minutes, taken out and cooled to room temperature, diluted with 5ml of water, and then placed in a water bath at 90°C to continue hydrolysis for 90 minutes;
[0154] (2) Derivation of test products:
[0155] Take out step (1) The hydrolyzed product is placed in an ice-water bath to cool, and then neutralized with 8mol / L sodium hydroxide solution, adjust the pH to neutral, then transfer to a 25ml volumetric flask to constant volume, take 1ml solution in a 10ml volumetric flask Add 5ml of 2mol / L sodium hydroxide solution and 5ml of 2mo...
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