Application of nano-ciprofloxacin particles in inhibiting biofilm formation of Pseudomonas aeruginosa
A technology of ciprofloxacin hydrochloride and nano rings, which is applied in the field of bio-nano, can solve problems such as treatment difficulties, and achieve the effects of low cytotoxicity, high drug loading rate, and stable loading system
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Embodiment 1
[0022] Example 1 Preparation of Nano Ciprofloxacin Particles
[0023] (1) CS is made into an aqueous solution with a concentration of 1.0 mg / ml with sterile double-distilled water, adjusted to pH 5.0 with NaOH, and sterilized by sterile microporous membrane filtration;
[0024] (2) S-β-CD is made into an aqueous solution with a concentration of 1.0 mg / ml with sterile double-distilled water, and is sterilized by microporous membrane filtration;
[0025] (3) Cip is made into an aqueous solution with a concentration of 1 mg / ml with sterile double-distilled water, sterilized by microporous membrane filtration, added to an equal volume of 1 mg / ml S-β-CD solution, and magnetically stirred at room temperature for 1 hour;
[0026] (4) Add an equal volume of 1 mg / ml CS aqueous solution, and continue to stir at room temperature for 4 h to obtain nano antibiotic drug-loaded particle emulsion. Centrifuge at 16000rpm for 30min, take the supernatant, measure the encapsulation efficiency, r...
Embodiment 2
[0028] Example 2 Preparation of empty nanoparticles
[0029] CS and S-β-CD with a concentration of 1.0 mg / ml were mixed according to the volume of 1:1, placed on a magnetic stirrer at room temperature and stirred for 4 hours, and the empty nanoparticle emulsion was obtained. Centrifuge at 16000rpm for 30min, rinse the precipitated particles three times with sterile water, and freeze-dry overnight to obtain empty nanoparticles (NPs).
Embodiment 3
[0030] Example 3 Detection of Ciprofloxacin Hydrochloride Concentration
[0031] (1) Prepare 10µg / ml Cip standard solution.
[0032] (2) According to the method in Table 1, dilute the 10 µg / ml Cip standard solution to obtain different concentrations, use distilled water as the blank control solution, and measure the absorbance value of each concentration of Cip at the maximum absorption wavelength of Cip at 276 nm with a UV-visible spectrophotometer . Draw a standard curve with the concentration as the X axis and the OD value as the Y axis. The regression equation is: y=0.109x-0.012 (R²=0.997), Y is the absorbance value, and X is the drug concentration (µg / ml).
[0033] Table 1 Cip standard curve drawing solution component preparation
[0034] Solution number 1 2 3 4 5 6 7 8 9 Cip standard solution (ml) 0.5 0.75 1.0 1.25 1.5 1.75 2.0 2.25 2.5 Distilled water (ml) 2.5 2.25 2.0 1.75 1.5 1.25 1.0 0.75 0.5 Cip final concentrat...
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