Hydrophilic modification method for PS-DVB (polystyrene-divinyl benzene) crosslinked microspheres grafted and bonded with PVA (polyvinyl alcohol)

Abstract

The invention relates to a hydrophilic modification method for PS-DVB (polystyrene-divinyl benzene) crosslinked microspheres grafted and bonded with PVA (polyvinyl alcohol). The method comprises steps as follows: epoxy groups with high reaction activity are introduced into surfaces of PS-DVB crosslinked microspheres and subjected to a reaction with hydroxyl in PVA, bonded PVA is subjected to a post-crosslinking reaction with a cross-linking agent, compact hydrophilic PS-DVB crosslinked microspheres wrapped with PVA are obtained, and hydrophobicity caused by benzene ring residues and pendent double bonds on the surfaces of the PS-DVB crosslinked microspheres is eliminated; furthermore, PVA is subjected to the post-crosslinking reaction, so that the phase collapse phenomenon of PVA long chains on the surfaces of the PS-DVB crosslinked microspheres in the field of bioseparation under the high-salt condition is avoided, further derivatization of a large amount of hydroxyl in PVA can be facilitated, chromatography separation media meeting different requirements can be prepared, and the method belongs to the field of modification of functional polymer microspheres.

Description

technical field [0001] The invention relates to a method for hydrophilic modification of PVA graft-bonded polystyrene-divinylbenzene crosslinked microspheres (PS-DVB microspheres), which is introduced on the surface of PS-DVB microspheres with high reactivity The epoxy group of the epoxy group reacts with the hydroxyl group in PVA, and the bonded PVA passes through the post-crosslinking reaction of the crosslinking agent to obtain PVA-coated compact hydrophilic PS-DVB microspheres, which eliminates the PS-DVB microsphere surface factor. The hydrophobicity caused by the residual benzene ring and the hanging double bond; and the post-crosslinking reaction of PVA can avoid the phase collapse phenomenon of the long chain of PVA on the surface of the hydrophilic PS-DVB microsphere under the high salt condition in the field of biological separation, and A large number of hydroxyl groups in PVA can facilitate further derivatization, and can be prepared into chromatographic separation...

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Problems solved by technology

Common polymers that can be used for bonding include PVA, PEG, and β-cyclodextrin, etc., mainly through the benzyl chloride or acetyl chloride (bromine) on the surface of functionalized PS-DVB microspheres and PVA (ZL200710177704.6, 2009 ), PEG (Journal of biomedicalmaterials research, 1992, 26, 779-790) and β-cyclodextrin (Journal of Functional Polymers, 1997, 10, 25-212) react under alkaline conditions, but benzyl chloride or ethyl Acyl chloride (bromine) has only 1-2 C atoms away from the benzene ring on the surface of PS-DVB microspheres. It reacts with large molecular weight polymers, and its benzyl chloride or acetyl chloride (bromine) has low degrees of freedom, and the reaction needs to It can only react under alkaline conditions, the reaction conditions are harsh, and the activity is low; in addition, Ma Guanghui and others grafted PVA to the surface of chloromethylated PS-DVB microspheres through etherification reaction in Chinese patent ZL 200710177704.6, but this method has Many problems, such as: the preparation process of chloromethylated PS-DVB microspheres requires the use of a strong carcinogen chloromethyl ether reagent; in addition, after PVA is grafted on the surface of PS-DVB microspheres, the PVA chain is too long, and it is easy to The surface is "entangled", and the hydrophilic PVA chain is too long without further treatment. During the bioseparation process, if the mobile phase has a high salt concentration, it is prone to phase collapse (Egbert Muller, Chemical Engineering & Technology, 2005, 28, 1292- 1305), which is not conducive to the separation of biological proteins, thus limiting its application range

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