Unlock instant, AI-driven research and patent intelligence for your innovation.

A method of using laser to induce myoglobin reduction

A myoglobin, laser-induced technology with applications in physiology

Active Publication Date: 2019-04-09
DALIAN UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the transformation of modern people's lifestyle, the acceleration of the pace of life and the aggravation of the concept of healthy eating, the traditional meat preservation can no longer meet people's needs, so the development of new technologies is required

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method of using laser to induce myoglobin reduction
  • A method of using laser to induce myoglobin reduction
  • A method of using laser to induce myoglobin reduction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0053] The present invention will be further explained below in conjunction with the accompanying drawings and specific embodiments.

[0054] A method of using laser to induce myoglobin reduction, comprising the following steps:

[0055] 1. Preparation of different forms of myoglobin and determination of spectral data, including steps:

[0056] (1) Prepare a certain amount of Na with a high concentration (0.2mol / L) of PH=7.4 2 HPO 4 -NaH 2 PO 4 The buffer solution is used as a stock solution and stored in a refrigerator at 4°C in the dark, and diluted to 0.05mol / L before use;

[0057] (2) Use prepared 0.05mol / L Na 2 HPO 4 -NaH 2 PO 4 Buffer solution to prepare high concentration (10 -4 mol / L) of pH = 7.4 metmyoglobin (MetMb) as a stock solution, and stored in a refrigerator at 4 ° C in the dark, and then diluted to 10 when ready to use -5 mol / L (the metmyoglobin used is purchased from the Sigma company of the United States);

[0058] (3) Use prepared Na 2 HPO 4 -N...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of physiology, particularly relates to reduction procedures of myoglobin in life bodies, and discloses a method for inducing reduction of myoglobin by the aid of laser light. Procedures of combination and separation of the myoglobin and oxygen in body fluid environments, change of structures of ferriporphyrin of a protein active center and electron transfer can be simulated. Research on change of valence states and coordination states of the ferriporphyrin in a site of the myoglobin active center is carried out by the aid of diversified spectral processes such as laser flash photolysis, ultraviolet-visible light and circular dichroism spectral processes. The method has the advantages that research on influence of amino acid on myoglobin transient absorption and dynamic procedures is carried out by the aid of laser flash photolysis technologies, effects different from effects of amino acid in proteins can be realized by the free amino acid as discovered in the research, and the free amino acid is high in influence on photo-excitation myoglobin relaxation procedures; the method has important physiological and medical significance, novel possibility can be provided for treating muscle and blood diseases by the aid of photodynamics, and novel ideas can be possibly provided for developing preservation technologies for meat products.

Description

technical field [0001] The invention belongs to the field of physiology, and specifically relates to the reduction process of myoglobin in a living body, the combination and separation of myoglobin and oxygen in a simulated body fluid environment, and the structure change of protein active center iron porphyrin and the process of electron transfer. technical background [0002] Proteins with heme as a prosthetic group are called hemoproteins. Hemoproteins can catalyze a series of reactions, from electron transfer, small molecule transport, sensing, to oxygen activation reactions. The heme prosthetic group constitutes the active center of this protein. Heme-like proteins have many important functions in organisms, such as storing and transporting O 2 , electron transfer, biocatalysis and small molecule biosensors (O 2 , NO and CO), etc. Due to the simple structure and important biological activity of myoglobin, myoglobin (Mb) has become a model protein for studying protein...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33G01N33/72
CPCG01N21/33G01N33/721
Inventor 曹洪玉史飞高凌星郑学仿唐乾
Owner DALIAN UNIV