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Pseudomonas deaza and its bacterial agent and their application in degrading quinclorac

A technology for denitrifying Pseudomonas bacteria and quinclorac, which is applied in the directions of bacteria, chemical instruments and methods, and the restoration of polluted soil, etc., can solve the adverse effects on tobacco growth and quality, economic losses in tobacco leaf production, and high implementation costs. problem, to achieve the effect of low cost, strong vitality and high degradation effect

Active Publication Date: 2019-09-03
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Quinclorac (Quinclorac) is a hormone-type quinolinic acid paddy field herbicide that was promoted by BASF in the 1980s. It has a good weeding effect especially for controlling barnyardgrass in rice fields, but it has a long residual period.
Therefore, quinclorac-based pesticide residues in the soil have adverse effects on the growth and quality of tobacco, and will bring serious economic losses to tobacco leaf production.
[0004] At present, the traditional treatment methods for pesticide residues in soil mainly include physical and chemical methods such as soil replacement, leaching, chemical passivation, electrical repair, thermal repair, etc., but generally speaking, these technologies have certain limitations. The cost of implementation is high, and the damage to the soil is serious, and it is easy to cause other pollution

Method used

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  • Pseudomonas deaza and its bacterial agent and their application in degrading quinclorac
  • Pseudomonas deaza and its bacterial agent and their application in degrading quinclorac
  • Pseudomonas deaza and its bacterial agent and their application in degrading quinclorac

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Effect test

Embodiment 1

[0051] This embodiment is used to illustrate the tolerance and degradation of Pseudomonas denitrification provided by the present invention to quinclorac in the aqueous phase (LB liquid medium)

[0052] Bacterial strain W-7 is inserted into the LB liquid culture of 30mg / L, 50mg / L, 100mg / L, 200mg / L and 300mg / L of quinclorac after sterilization with the inoculation amount of 1 volume % Base. Cultured at 170rpm, 30±1°C for 24h, the results showed that the strain W-7 showed good tolerance to quinclorac, and the strains could grow in different media. The maximum OD value that the bacteria can reach and the degradation rate of quinclorac are shown in Table 1.

Embodiment 2

[0060] This embodiment is used to illustrate the tolerance and degradation of Pseudomonas denitrifica provided by the present invention to quinclorac in the aqueous phase (inorganic salt medium)

[0061] Inoculate bacterial strain W-7 with the inoculum amount of 1 volume % to contain the inorganic salt culture of the quinclorac of 30mg / L, 50mg / L, 100mg / L, 200mg / L and 300mg / L respectively after sterilizing Base. Cultivate at 170rpm, 30±1°C for 24h. The results showed that the strain W-7 showed good tolerance to quinclorac, and the strains could grow in different media. In the case of using quinclorac as the only carbon source, the maximum OD value that the bacteria can achieve and the degradation rate of quinclorac are shown in Table 1.

Embodiment 3

[0072] This embodiment is used to illustrate the degradability of Pseudomonas denitrification provided by the present invention to quinclorac in soil

[0073]Bacterial strain W-7 was inoculated into 100mL of LB liquid culture medium with 1 volume % strain amount, after 175rmp, 30 ± 1 ℃ under cultivation 24h, the bacterial solution was added to the pollution of 5kg containing 20mg / kg quinclorac In the soil, stir evenly, cultivate for 7 days, and ensure that the water content in the soil is about 20%, and detect the content of quinclorac in the soil on the 3rd day, the 5th day and the 7th day respectively, and the results are shown in Table 2.

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Abstract

The invention discloses pseudomonas denitrificans having the preservation number of CGMCC No.10845. The invention also discloses a microbial inoculum comprising the pseudomonas denitrificans. The invention further discloses an application of the pseudomonas denitrificans and the microbial inoculum in degradation of quinclorac. The invention also discloses a method for degradation of quinclorac. The pseudomonas denitrificans is characterized in that: (1) only an inorganic salt culture medium is provided, and the pseudomonas denitrificans can survive and grow in a culture environment containing quinclorac as a sole carbon source, and has the advantages of strong vital force, fast growth speed, and large living weight; (2) during preparation, only a preservation strain needs to be activated, the operation is simple and the cost is low; and (3) the pseudomonas denitrificans has high degradation effect in water bodies and soil, in the water bodies, under a condition that the content of quinclorac is 200 mg / L, the bacteria are cultured for 24 h, the degradation rate is as high as 93.4%, and in the soil, under a condition that the content of quinclorac is 20 mg / kg, the degradation rate is as high as 88.0%.

Description

technical field [0001] The present invention relates to the field of environmental remediation of microorganisms, specifically, a strain of Pseudomonas denitrificans with quinclorac degrading function, bacterial agents containing the Pseudomonas denitrificans, and their degradation function Application of quinclorac. Background technique [0002] Quinclorac (Quinclorac) is a hormone-type quinolinic acid paddy field herbicide promoted by BASF in the 1980s. It has a good herbicidal effect especially for controlling barnyardgrass in rice fields, but it has a long residual period. Its chemical name is 3,7-dichloroquinoline-8-carboxylic acid and its molecular formula is C 10 h 5 C l2 NO 2 , with a molecular weight of 942.1 Da, and its trade names are Kebaixing, Kuaishabai, Shenhoe, Baicaojing, Shabailing, Barnyardgrass King, etc. Its target is the synthetic hormone in plants. By interfering with plant hormones to regulate enzyme activity, the growth and metabolism of organis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/38C02F101/38
Inventor 任立伟齐丹林森王培红
Owner 粮华生物科技(北京)有限公司
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