Preparation method for antimicrobial peptide formula for dental implant

A technology for dental implants and dental implants is applied in the field of preparation of antimicrobial peptide formulations, which can solve the problems of implant loosening and falling off, peripheral bone tissue defect and loss, etc., and achieves broad application prospects and high-efficiency sterilization effects.

Inactive Publication Date: 2017-03-22
DALIAN SANSHENG SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Peri-implantitis can lead to the defect and loss of bone tissue around the implan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1

[0028] (1) The dental implant with the surface of the titanium dioxide nanotube array layer was cleaned with deionized water, ethanol and acetone in sequence, and then dried with nitrogen, placed in a vacuum drying oven, and dried at 60°C for 24 hours , Put aside;

[0029] (2) Dissolving chitosan in a 0.3% acetic acid solution to prepare a 0.5 mg / ml chitosan solution. The chitosan has a degree of deacetylation of 50% and Mw of 1000 kDa.

[0030] (3) Mix the solution obtained in step (2) with liquid paraffin at a volume ratio of 0.1, and add Span 80 with a volume concentration of 0.5%. At the same time, add the dispersing agent magnesium stearate, the combination of Span 80 and magnesium stearate The mass ratio is 2:1. Stir for more than 1 hour at room temperature to form a uniform water-in-oil emulsion;

[0031] (4) Add 1 ml of the crosslinking agent vanillin at 5 mg / ml to the water-in-oil emulsion obtained in step (3), and react for 4 hours under stirring at 40°C ...

Example Embodiment

[0034] Example 2

[0035] (1) The dental implant with the surface of the titanium dioxide nanotube array layer was cleaned with deionized water, ethanol and acetone in sequence, and then dried with nitrogen, placed in a vacuum drying oven, and dried at 60°C for 24 hours , Put aside;

[0036] (2) Dissolve chitosan in a 3% acetic acid solution to prepare a 5 mg / ml chitosan solution. The chitosan has a degree of deacetylation of 98% and a Mw of 1500 kDa.

[0037] (3) Mix the solution obtained in step (2) with liquid paraffin at a volume ratio of 0.4, and add Span 80 with a volume concentration of 1%. At the same time, add the dispersing agent magnesium stearate, the blend of Span 80 and magnesium stearate The mass ratio is 4:1, stirring at room temperature for more than 1 hour to form a uniform water-in-oil emulsion;

[0038] (4) Add 1 ml of 10mg / ml crosslinker vanillin to the water-in-oil emulsion obtained in step (3), and react for 6 hours under stirring at 60°C to obtain chitosan par...

Example Embodiment

[0041] Example 3

[0042] The dental implant obtained in step (1) of Example 1 was immersed in the chitosan solution described in Examples 1 and 2 above for 10-20 minutes to make the surface of the dental implant positively charged; Fully clean with ionized water;

[0043] Immerse the above-mentioned cleaned dental implant in the 0.5mg / ml sodium hyaluronate solution mentioned above for 10-20 minutes, so that the surface of the dental implant will be negatively charged; then fully rinse with deionized water;

[0044] The dental implant obtained by the treatment is immersed in the chitosan solution described in Examples 1 and 2 above for 10-20 minutes, so that the surface of the dental implant is positively charged; then it is thoroughly washed with deionized water;

[0045] According to the number of layers to be assembled, repeat the steps of Example 3 more than two times to obtain dental implants with different layers of coating.

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Abstract

The invention discloses a preparation method for an antimicrobial peptide formula for a dental implant, especially for the dental implant with different layers of coatings. The coatings are arranged as molecular coatings which are formed and sequentially arrayed on the solid surface of the dental implant under the electrostatic attraction effect between positive ions and negative ions. Chitosan and hyaluronic acid coatings are made from the biological materials with excellent biological degradability and biocompatibility. Due to the existence of a surface chondroitin sulfate layer-antimicrobial peptide layer compound structure, the antimicrobial peptide formula has the advantages of long-lasting effect, efficient sterilization, anti-infection capability, anti-microbial and anti-apoptosis activity, capability of supporting conglutination, survival, growth and differentiation of various cells (such as osteoblast and chondrocyte) and wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of dental implants, and in particular relates to a preparation method of an antibacterial peptide formula for dental implants. Background technique [0002] With the continuous development of dental implant technology and wide recognition by patients, it is also the fastest-growing specialty in stomatology in recent years. It has continuously made breakthroughs and is providing professional help to more patients with dental diseases. However, another current situation that has to be admitted is that the existing interventional devices are prone to infection after being implanted in the body. The main infection mechanism is that the bacteria entering the body first adhere to the surface of the material, and due to the rich nutrient environment in the body, the bacteria reproduce and form a protective biofilm. The presence of biofilms makes infections from medical devices particularly difficult to treat. [...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/54A61L27/02A61L27/50
CPCA61L27/34A61L27/025A61L27/50A61L27/54A61L2300/404A61L2420/08A61L2430/12C08L5/08
Inventor 谢红国马小军张久文蔡俊江彭鹏李闪
Owner DALIAN SANSHENG SCI & TECH DEV
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