Bacterial cellulose/fullerene composite material and preparation method thereof
A technology of bacterial cellulose and composite materials, which is applied in the field of biomedical materials, can solve the problems of low solubility and limit applications, and achieve the effects of overcoming damage to the human body, excellent mechanical properties, and good biocompatibility
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Embodiment 1
[0030] Step 1, preparation of bacterial cellulose
[0031] Preparation method: Inoculate the Acetobacter xylinum species into the seed medium prepared with fruit juice, high biological nutrients and trace elements, shake and culture at 200rmp and 27°C for 18h to obtain the culture solution. Shake the culture solution evenly, place it in an incubator at 28°C for cultivation, and the bacterial cellulose can be obtained after the culture solution is completely consumed.
[0032] The composition of the seed medium of the present embodiment is: 500ml of coconut juice or pineapple juice contains 8g of glucose, 3g of yeast extract powder, 2g of peptone, 0.1g of magnesium sulfate, and 0.05g of anhydrous calcium chloride.
[0033] Step 2, purification of bacterial cellulose
[0034] The bacterial cellulose prepared in step 1 is fully cleaned with ultrapure water to remove surface impurities, then placed in 0.1mol / L sodium hydroxide aqueous solution and boiled for 0.5h, then soaked in ...
Embodiment 2
[0045] Step 1, preparation of bacterial cellulose
[0046] Preparation method: Inoculate Acetobacter xylinum strains into the seed medium, shake and culture at 150rmp and 28°C for 24h to obtain a culture solution. Shake the culture solution evenly, place it in an incubator at 28°C for cultivation, and the bacterial cellulose can be obtained after the culture solution is completely consumed.
[0047]The composition of the seed medium of the present embodiment is: 500ml of coconut juice or pineapple juice contains 16g of glucose, 8g of yeast extract powder, 5g of peptone, 1.0g of magnesium sulfate, and 0.5g of anhydrous calcium chloride.
[0048] Step 2, purification of bacterial cellulose
[0049] Fully wash the bacterial cellulose prepared in step 1 with ultrapure water to remove surface impurities, then boil it in 0.2 mol / L aqueous sodium hydroxide solution for 1 hour, then soak it in ultrapure water for 4 days, every 8 hours Change the water once until it is neutral, and t...
Embodiment 3
[0057] Step 1, preparation of bacterial cellulose
[0058] Preparation method: Inoculate the Acetobacter xylinum species into the seed medium, shake and culture at 300rmp and 30°C for 48h to obtain the culture solution. Shake the culture solution evenly, place it in an incubator at 28°C for cultivation, and the bacterial cellulose can be obtained after the culture solution is completely consumed.
[0059] Step 2, purification of bacterial cellulose
[0060] The bacterial cellulose prepared in step 1 is fully washed with ultrapure water to remove surface impurities, then placed in 0.3mol / L sodium hydroxide aqueous solution and boiled for 0.5h, then soaked in ultrapure water for 2 days, every Change the water once every 12 hours until neutral, and store the purified bacterial cellulose in a 4°C refrigerator for later use.
[0061] Step 3, preparation of fullerene aqueous dispersion
[0062] Take 15mgC 70 Add 700ml of tetrahydrofuran and stir for 48h under the protection of a...
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