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A kind of Enterobacter ludwig and its application in inducing magnesium ion mineralization

A technology of Enterobacter ludwigii and Enterobacter dervii, applied to Enterobacter ludwigii and its application field in inducing magnesium ion mineralization, can solve the problems of few reports of spherochite, etc., Achieving the effect of low price, obvious settlement effect and environmental friendliness

Active Publication Date: 2019-03-26
SHANDONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Balloonite, also known as Dupinite (Mg 5 (CO 3 ) 4 (OH) 2 ·5H 2 O), is a flower-like mineral, often associated (para)growth with magnesite, but there are few reports on venerene

Method used

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  • A kind of Enterobacter ludwig and its application in inducing magnesium ion mineralization
  • A kind of Enterobacter ludwig and its application in inducing magnesium ion mineralization
  • A kind of Enterobacter ludwig and its application in inducing magnesium ion mineralization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Isolation, Identification and Preservation of Enterobacter ludwigii SYB1CGMCC No.12709

[0065] 1. Isolation of sulfate-reducing bacteria SYB1

[0066] 1. Add 10g of rock powder sample (collected from the dolomite of Mantou Mountain in Changqing, Jinan, China) to 100mL of sterile distilled water, stir evenly, let stand for 10 minutes, then take 20mL supernatant and add it to 200mL enrichment medium, and use Seal the liquid level in the bottle with sterile liquid paraffin, place it in a constant temperature incubator at 37°C for static cultivation, and observe. After culturing until the 7th day, the color of the enrichment medium turned black, indicating that the sulfate-reducing bacteria were successfully enriched (Fe(NH in the enrichment medium 4 ) 2 (SO 4 ) 2 The ferrous ions in the enrichment medium can interact with the S in the 2- The reaction produces black ferrous sulfide).

[0067] 2. After completing step 1, take the supernatant and inoculate it to a conc...

Embodiment 2

[0080] Preparation of Enterobacter ludwig SYB1 bacterial agent

[0081] Activate Enterobacter ludwig SYB1 on the solid purification medium, then pick a single colony and inoculate it into a 250mL Erlenmeyer flask filled with 100mL liquid purification medium, and culture it on a constant temperature shaker at 37°C and 130r / min for 1 day , get OD 600 Enterobacter ludwig SYB1 agent A with a value of approximately 1.80. Enterobacter ludwig SYB1 agent A is hereinafter referred to simply as SYB1 agent A.

Embodiment 3

[0083] Characterization of Enterobacter ludwigii SYB1

[0084] This example studies the growth curve and pH change curve of Enterobacter ludwig SYB1 under different Mg / Ca ratios.

[0085] Use each medium (calcium-magnesium-containing medium A, calcium-magnesium-containing medium B, calcium-magnesium-containing medium C, calcium-magnesium-containing medium D and calcium-magnesium-containing medium E) to carry out the following operations:

[0086] Get 6 250mL Erlenmeyer flasks, each Erlenmeyer flask is filled with 150mL medium, divide 6 Erlenmeyer flasks into experimental group and control group, each group has 3 Erlenmeyer flasks; inoculate the Erlenmeyer flasks of the experimental group Inoculate 1.5 mL of SYB1 bacterial agent A into the Erlenmeyer flask of the control group with an equal volume of sterile ultrapure water; after the inoculation, place the Erlenmeyer flask in a constant-temperature shaking incubator and incubate at 37°C and 130r / min. Sampling 4mL at the end o...

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Abstract

The present invention relates to a strain of Enterobacter ludwigii, which is classified as Enterobacter ludwigii SYB1, and has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on June 27, 2016, and the preservation number is CGMCC No.12709. The present invention also relates to the application of Ludwig enterobacteria strain in inducing magnesium ion mineralization. Enterobacter ludwig strain of the present invention is to Mg 2+ The sedimentation effect is remarkable, and high-quality magnesite can be prepared by using the Enterobacter ludwig strain of the invention, and can also be used in the field of hard water softening, with good sedimentation effect, simple operation, low price, and environmental friendliness.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to an enterobacter ludwig and its application in inducing magnesium ion mineralization. Background technique [0002] Magnesite, also known as magnesite or alkali magnesite, is a carbonate mineral of magnesium, and its main chemical composition is magnesium carbonate (MgCO 3 ). After processing, magnesite is mainly used as a refractory material for industrial high-temperature furnaces and equipment in terms of high temperature resistance and corrosion resistance. In addition, it is also used in building materials, chemical industry, agriculture and animal husbandry, papermaking, aerospace, automobile and environmental protection and other industries. Moreover, magnesite is the basic raw material for the production of alkaline refractory materials and one of the three pillars of refractory raw materials in my country. At present, the national magnesia material industry has developed ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P3/00C12R1/01
CPCC12P3/00C12R2001/01C12N1/205Y02A50/30
Inventor 韩作振孟瑞瑞闫华晓赵辉韩梅赵延洋孙彬庄定祥李丹刘斌为
Owner SHANDONG UNIV OF SCI & TECH
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