Multiple RT-PCR detection kit for identifying PRRSV (Porcine Reproductive and Respiratory Syndrome)

Abstract

The invention discloses a multiple RT-PCR detection kit for identifying PRRSV (Porcine Reproductive and Respiratory Syndrome), and a specific amplification primer which is used for the kit and aims at a PRRSV classic strain, a high pathogenicity variant strain and a high pathogenicity vaccine strain (JXA1-R strain). The kit can be used for detecting the PRRSV and distinguishing different toxic strain types (the classic strain, the high pathogenicity variant strain and the high pathogenicity vaccine strain JXA1-R strain). The invention provides a method for utilizing the kit for detecting the PRRSV at the same time, so that the PRRSV can be quickly and specifically detected, and the toxic strain types can be quickly and specifically distinguished. The method provided by the invention has the characteristics of quickness, simplicity, convenience, high specificity and high sensibility, can be used for large-batch sample detecting at the same time, can distinguish and detect whether a sample is infected by the PRRSV classic strain, the PRRSV high pathogenicity variant strain or the PRRSV high pathogenicity vaccine strain JXA1-R strain or is jointly infected by two or three of the PRRSV classic strain, the PRRSV high pathogenicity variant strain and the PRRSV high pathogenicity vaccine strain JXA1-R strain through one-time reaction, can provide a technical support for epidemic surveillance, epidemiological investigation and synthesize prevention and control of the PRRSV, and has a favorable application prospect.

Description

technical field [0001] The invention belongs to the technical field of animal virus molecular biology, and specifically relates to a multiple RT-PCR detection kit for differentiating and detecting PRRSV classic strains, highly pathogenic variant strains and highly pathogenic vaccine strains (JXA1-R strains). In clinical or scientific research, it is used to identify whether the test sample is infected by the classic strain of PRRSV, or infected by the highly pathogenic variant strain of PRRSV, or infected by the highly pathogenic vaccine strain of PRRSV (JXA1-R strain), or both or co-infection of the three. Background technique [0002] Porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, PRRS) is caused by porcine reproductive and respiratory syndrome virus (Porcine reproductive and respiratory syndrome virus, PRRSV), with fever, abortion, and death of piglets before and after weaning Diseases with clinical features such as elevated...

AI Technical Summary

Problems solved by technology

[0005] There are many traditional diagnostic methods for porcine reproductive and respiratory syndrome, such as virus isolation, immunohistochemistry, and immunofluorescence tests for the detection of pathogens, and ELISA, serum virus neutralization test, and indirect immunofluorescence test for the detection of antibodies. , immunoperoxidase monolayer test and other serological methods, but these diagnostic methods have limitations such as low sensitivity, poor specificity, long time, and inability to distinguish infection by different strains of PRRSV to varying degrees.

The RT-PCR method for all PRRSV has been established at home and abroad, but this method can only detect whether there is PRRSV infection, and cannot judge whether it is a classic PRRSV infection, or a highly pathogenic variant of PRRSV infection, or both co-infection

In addition, the existing RT-PCR detection method for highly pathogenic variants of PRRSV can only detect highly pathogenic variants of PRRSV, and cannot determine whether it is a single highly pathogenic variant of PRRSV or a classic strain of PRRSV. , highly pathogenic variant strains, or co-infection with highly pathogenic vaccine strains

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