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Preparation method for collagen-hydroxyapatite scaffold containing nano silver sulfide

A hydroxyapatite and silver sulfide technology, applied in microcapsules, capsule delivery, pharmaceutical formulations, etc., can solve the problems of no benefit in wound repair, non-healing wounds, delayed healing, etc., and achieve easy promotion, simple materials, and production cycle. short effect

Inactive Publication Date: 2017-03-29
王淑芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the high biological activity of nano-silver sulfide, when it is applied to tissue engineering, it will be released to the wound surface or target organ, and it will be preferentially captured by related proteins, chloride ions and groups in the wound exudate or tissue, resulting in nano-silver sulfide Particles are abnormally aggregated at high concentrations in certain parts. Although they can effectively participate in antibacterial activities, these excess nano-silver sulfide will accumulate in epidermal cells, macrophages, and fibroblasts, leading to cell apoptosis and eventually non-healing of the wound. or delayed healing
In general, unrestrained use or uncontrolled release of nano-silver sulfide is bound to be counterproductive and not beneficial to wound repair

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method of a collagen-hydroxyapatite scaffold containing nano-silver sulfide is achieved through the following processes:

[0019] (1) Adopting silver nitrate and dioctadecyl dithiophosphoric acid and adding dipotassium hydrogen phosphate as a dispersant to carry out liquid phase reduction reaction to prepare nano-silver sulfide sol; the addition of dipotassium hydrogen phosphate is the quality of tetrachloroauric acid 3%; the reaction temperature is 40°C;

[0020] (2) Take the nano-silver sulfide sol and stir for 10 minutes, heat it under microwave under airtight conditions (select Beijing Xianghu HX-300A computer microwave ultrasonic combined synthesizer as the microwave source), first react at 300W microwave power for 2.25min, and then at 120W React under microwave power for 2.75min to obtain nano-silver sulfide solution;

[0021] (3) Dissolve type Ⅰ collagen and hydroxyapatite derived from bovine Achilles tendon into 0.5mol / L acetic acid solution at a ...

Embodiment 2

[0023] A preparation method of a collagen-hydroxyapatite scaffold containing nano-silver sulfide is achieved through the following processes:

[0024] (1) Adopting silver nitrate and dioctadecyl dithiophosphoric acid and adding dipotassium hydrogen phosphate as a dispersant to carry out liquid phase reduction reaction to prepare nano-silver sulfide sol; the addition of dipotassium hydrogen phosphate is the quality of tetrachloroauric acid 3%; the reaction temperature is 30°C;

[0025] (2) Take the nano-silver sulfide sol and stir for 10 minutes, and heat it under microwave under airtight conditions (select Beijing Xiangyu HX-300A computer microwave ultrasonic combined synthesizer as the microwave source), first react at 250W microwave power for 2.5min, and then at 150W React under microwave power for 2.5min to obtain nano-silver sulfide solution;

[0026] (3) Dissolve type Ⅰ collagen and hydroxyapatite derived from bovine Achilles tendon into 0.5mol / L acetic acid solution at ...

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Abstract

The invention relates to a preparation method for a collagen-hydroxyapatite scaffold containing nano silver sulfide. The method comprises the steps of preparing nano silver sulfide through a liquid reduction reaction; uniformly mixing nano silver sulfide with a collagen-hydroxyapatite solution; performing freeze-drying by a one-step process; and performing cross-linking to obtain the collagen-hydroxyapatite scaffold, wherein the concentration range of the nano silver sulfide is 10-50ug / ml, and the particle sizes of nano silver sulfide particles are 10-30nm. According to the scaffold, the required materials are simple; complex technical requirements and high-end equipment demands are not needed; the scaffold is low in cost and easy to popularize; a reasonable nano silver sulfide sustained release system is provided for a wound surface; long-term effective drug sustained release is provided for the wound surface; and the drug concentration of the wound surface is kept balanced, effective and non-excessive.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a preparation method of a collagen-hydroxyapatite scaffold containing nano-silver sulfide. Background technique [0002] Hydroxyapatite and collagen are the most important inorganic and organic components in natural bone, and both have good biological properties, but they have different defects when used alone, and it is difficult to meet the performance requirements of tissue engineering for biomaterials. The combination of collagen and hydroxyapatite makes better use of the cohesiveness of collagen and overcomes the fluidity of hydroxyapatite particles. [0003] Nano-silver sulfide has excellent thermal conductivity, electrical conductivity, surface activity and catalytic performance, and has great application value in the fields of optics, catalysis, microelectronics, biosensing, and antibacterial. Nano-silver sulfide powders with different shapes have differ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/44A61L27/56A61L27/54
CPCA61L27/46A61L27/446A61L27/54A61L27/56A61L2300/102A61L2300/404A61L2300/622
Inventor 王淑芳
Owner 王淑芳