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Halotolerant nitrate reducing bacteria, and applications thereof

A nitrate and salt-tolerant technology, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve problems such as loss, and achieve the effect of low cost and simple operation

Active Publication Date: 2017-03-29
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of denitrification is to cause the loss of N and thus reduce the biological denitrification

Method used

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  • Halotolerant nitrate reducing bacteria, and applications thereof
  • Halotolerant nitrate reducing bacteria, and applications thereof
  • Halotolerant nitrate reducing bacteria, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Cultivation, isolation, screening, identification and preservation of nitrate-reducing bacteria Roseovarius lacus GSS12

[0011] 1.1 Culture and isolation of strains

[0012] The nitrate-reducing bacterium Roseovarius lacus GSS12 of the present invention is obtained by separating and purifying the bottom sludge of the salt lake in Yuncheng, Shanxi.

[0013] Concrete separation and purification method comprises the following steps:

[0014] (1) Take 5.0g of Shanxi Yuncheng Salt Lake bottom mud and inoculate it into the enrichment and separation medium with a pH value of 7.2, culture at 30°C with shaking at 200rpm / min; after culturing for 3 days, transfer the culture medium to Transfer to another fresh enrichment and separation medium, and culture with shaking at 30°C and 200rpm / min. Such enrichment culture for four generations.

[0015] (2) Dilute the culture solution after the fourth generation reaction to 10-5-10-6 by the dilution plate method, spread 0.1...

Embodiment 2

[0031] Embodiment 2: the preparation of microbial bacterial agent

[0032](1) Shake flask culture: Pick a ring of bacterial lawn from the R. lacus GSS12 slant and transfer it into an Erlenmeyer flask filled with 100mL 2216E liquid medium, and cultivate it on a shaking table at 37°C and 200rpm / min for 24h. The formula of the 2216E liquid medium described is: yeast powder 1.0g, peptone 5.0g, ferric citrate 0.1g, NaCl 19.45g, MgCl 2 5.98g, Na 2 SO 4 3.24g, CaCl 2 1.8g, KCl 0.55g, Na 2 CO 3 0.16g, BrCl 2 0.08g, SeCl 2 0.034g, H 3 BO 3 0.022g, Na 2 SiO 3 0.004g, NaF 0.0024g, NH 4 NO 3 0.0016g, Na 2 HPO 4 0.008g, water 1000mL, pH 7.2.

[0033] (2) Fermentation in seed tank: put the cultured R. lacus GSS12 fermentation broth into a 10L fermenter, and ferment at 37°C and 200rpm / min for 36 hours; the formula of the fermentation medium used is the above-mentioned liquid medium.

[0034] (3) Fermentation in a fermenter: put the cultured R. lacus GSS12 fermentation...

Embodiment 3

[0036] Embodiment 3: the preparation of microbial bacterial agent

[0037] (1) Shake flask culture: Pick a ring of bacterial lawn from the R. lacus GSS12 slant and transfer it into an Erlenmeyer flask filled with LB liquid medium, culture at 50°C and 240rpm / min on a shaker for 24 hours. The formula of the 2216E liquid medium described is: yeast powder 1.0g, peptone 5.0g, ferric citrate 0.1g, NaCl 19.45g, MgCl 2 5.98g, Na 2 SO 4 3.24g, CaCl 2 1.8g, KCl 0.55g, Na 2 CO 3 0.16g, BrCl 2 0.08g, SeCl 2 0.034g, H 3 BO 3 0.022g, Na 2 SiO 3 0.004g, NaF0.0024g, NH 4 NO 3 0.0016g, Na 2 HPO 4 0.008g, water 1000mL, pH 7.2.

[0038] (2) Fermentation in seed tank: put the cultured R. lacus GSS12 fermentation broth into a 10L fermenter, and ferment at 30° C. and 200 rpm / min for 24 hours; the formula of the fermentation medium used is the above-mentioned liquid medium.

[0039] (3) Fermentation in a fermenter: put the cultured R. lacus GSS12 fermentation broth into a f...

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Abstract

The invention discloses a halotolerant nitrate reducing bacteria, and applications thereof. The halotolerant nitrate reducing bacteria is named as Roseovarius lacus, and is preserved in Guangdong culture collection center, 5th floor, building NO.59, yard NO.100, Xianlie zhonglu, Guangzhou. The preservation number is GDMCC No.60066. The halotolerant nitrate reducing bacteria is capable of reducing nitrate nitrogen into nitrites at high salinity environment, reducing the nitrites into nitrogen further, and realizing bioconversion of the nitrates, and can be widely applied in the field of waste water biological denitrification.

Description

technical field [0001] The invention relates to a new bacterial strain and its application in the field of environmental engineering, in particular to a salt-tolerant nitrate-reducing bacterium and its application. Background technique [0002] The nitrogen cycle is an important biogeochemical process and the most important mineral-inorganic nutrient for microorganisms and plants. Nitrogen is absorbed in the form of inorganic salts and circulated in a redox manner. The ammonium-containing nitrogen fertilizer used in the agricultural system and the daily excrement of animals in the feedlot will cause excessive nitrogen in the soil or groundwater environment, and the ammonia nitrogen will be converted into nitrate under the action of nitrifying bacteria. Nitrate, as a stable final oxidation product, accumulates in the environment under natural conditions and has potential impacts on the health of humans, animals and plants. [0003] Nitrate reduction is the first stage react...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/163C12N1/205C12R2001/01
Inventor 王跃强余震庄莉汤佳潘家峰
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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