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Preparing method of authopleura midori uchida muramatsu resisting prostatic cancer oligopeptides

An anti-prostate cancer and anti-prostate anemone technology, which is applied in the field of preparation of anti-prostate cancer oligopeptides of the green side anemone, can solve problems such as difficulty in treatment, and achieve the effects of easy process control, good safety, and high purity

Active Publication Date: 2017-04-12
ZHEJIANG OCEAN UNIV
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  • Abstract
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Problems solved by technology

Without timely and effective treatment, it will develop into androgen-independent prostate cancer, and advanced prostate cancer plus bone metastasis will bring great difficulty to treatment

Method used

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  • Preparing method of authopleura midori uchida muramatsu resisting prostatic cancer oligopeptides
  • Preparing method of authopleura midori uchida muramatsu resisting prostatic cancer oligopeptides
  • Preparing method of authopleura midori uchida muramatsu resisting prostatic cancer oligopeptides

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Embodiment

[0029] 1. Materials

[0030] 1.1 Raw materials

[0031] Anthopleura anjunae: The wild sea anemone collected from Zhoushan sea area was identified as Anthopleura anjunae by Professor Zhao Shenglong from Zhejiang Ocean University.

[0032] 1.2 Reagents and Instruments

[0033] Alkaline protease, trypsin, neutral protease, pepsin: Asia Pacific Hengxin Biotechnology Co., Ltd.; methanol, acetonitrile: chromatographically pure, Merck, Germany; polybrene: Shanghai Bopu Biotechnology Co., Ltd.; F12 medium: the United States Gibco; mycoplasma-free fetal bovine serum (FBS): Hangzhou Sijiqing Biological Products Co., Ltd. Human prostate cancer cell line DU-145 was purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences.

[0034] CF16RN high-speed refrigerated centrifuge, L8900 amino acid automatic analyzer: Hitachi Instrument Co., Ltd.; Agilent-1260 high-performance liquid chromatography: Agilent Corporation of the United States; ALPHA1-4 / LDplus ultrafiltration ...

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Abstract

The invention discloses a preparing method of authopleura midori uchida muramatsu resisting prostatic cancer oligopeptides. The preparing method comprises the steps of preprocessing of a sample, enzymolysis, ultrafiltration, anion exchange chromatography, Sephade*G25 gel chromatography, separation and purification of reversed-phase high-performance liquid chromatography, and the final obtaining of the authopleura midori uchida muramatsu resisting prostatic cancer oligopeptides. According to the authopleura midori uchida muramatsu resisting prostatic cancer oligopeptides, the safety is good, the reaction condition is mild, process control is easy, the purity of the obtained authopleura midori uchida muramatsu resisting prostatic cancer oligopeptides is high, the authopleura midori uchida muramatsu resisting prostatic cancer oligopeptides have obvious anti-proliferative effects on prostate cancer cell DU-145, and the authopleura midori uchida muramatsu resisting prostatic cancer oligopeptides have good medical application value.

Description

technical field [0001] The invention relates to the technical field of extracting marine active substances, in particular to a method for preparing an anti-prostate cancer oligopeptide of Pleurotus chinensis. Background technique [0002] Sea anemone (Sea anemone) belongs to primitive marine organisms, an order of the subclass Hexacoral, and there are 37 species in six families. It is widely distributed and is commonly found in tropical and temperate seabed rocks and sediments. At present, scientists from all over the world have done a lot of research on the activity of potassium ion channel and sodium ion channel of sea anemone toxin; in addition, there are also a lot of reports on its blood pressure lowering, antifungal and antitumor research. However, due to the extremely small content of sea anemone toxin and the difficulty of extraction, the research on its physiology and pathology is seriously restricted. Referring to the research progress of marine bioactive peptides...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16
CPCC07K1/16C07K1/18C07K1/20C07K1/34C07K1/36C12P21/06
Inventor 丁国芳吴宗泽杨最素贾盈露郑媛媛陈锐余方苗
Owner ZHEJIANG OCEAN UNIV
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