Method for biologically synthesizing natural aromadendrin by escherichia coli through utilizing naringenin

A technology of Escherichia coli and naringenin is applied in the field of fermentation engineering to achieve the effects of simple production process, low production cost and simple composition
CN106635892AInactive Publication Date: 2017-05-10WUXI NEWWAY FERMENTATION TECH RES INST
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
WUXI NEWWAY FERMENTATION TECH RES INST
Publication Date
2017-05-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for biologically synthesizing natural aromadendrin by escherichia coli through utilizing naringenin and belongs to the field of fermentation engineering. The method takes a gene engineering strain capable of producing the aromadendrin as a starting strain; a relatively high thalli concentration is obtained by utilizing glucose and an organic nitrogen source, and exogenous proteins are induced to express through an inducer; finally, efficient biological synthesis of the aromadendrin is realized. The production method disclosed by the invention can realize high-efficiency production of the natural aromadendrin; within a fermentation period, namely 55h, the concentration of the aromadendrin in a fermentation solution reaches 3g / L to 5g / L and the substrate mass conversion rate reaches 70 percent to 90 percent.
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Description

technical field

[0001] The invention relates to a method for Escherichia coli to biosynthesize natural orangenin by utilizing naringenin, and belongs to the field of fermentation engineering. Background technique

[0002] Aromadendrine, also known as Dihydrokaempferol, is a flavonol, which belongs to flavonoids and is an important precursor of plant anthocyanins. Cougarin was discovered from peach for the first time, and has since been found to exist in different plants, such as plants of the genus Tassel, Dionysus chrysanthemum, goat tree, arborvitae leaves and Siberian red pine, etc., but it is distributed in nature Not widely.

[0003] Flavonoids have been widely reported to have various pharmacological activities such as anti-inflammatory, anti-oxidation, anti-tumor, and neuroprotective activities. There have been many reports showing that tangerin has various biological activities, such as anti-inflammatory activity, free radical scavenging activity and anti-tumor act...

Claims

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