Spirulina culture method
A breeding method and technology of spirulina, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of increasing algae biomass concentration, promoting photosynthetic productivity, and inhibiting bacterial reproduction
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Embodiment 1
[0031] A kind of cultivation method of spirulina of the present invention comprises the steps:
[0032] (1) Put the solid culture medium in a sterilizing pot and cook for 90 minutes, keep the temperature at 120°C and the air pressure at 0.01MPa, and cool the cooked medium to 32°C for use;
[0033] The solid medium is composed of A component and B component, and the A component is composed of the following components by mass percentage:
[0034]
[0035]
[0036] The B component is underground seawater, and the mass ratio of the B component to the A component is 2:1; the preparation method of the solid medium: weigh the A component and the B component in the above mass percentage, and A component is added into underground seawater with a salinity of 30wt‰ and stirred evenly to obtain a solid medium. The mass of the underground seawater accounts for 60% of the total mass of the components, and the underground seawater is filtered with a double layer of 250-mesh cotton clo...
Embodiment 2
[0042] The difference between embodiment 2 and embodiment 1 is: a kind of cultivation method of spirulina of the present invention comprises the steps:
[0043] (1) Put the solid culture medium in a sterilizing pot and cook for 100 minutes, keep the temperature at 122°C, and the air pressure at 0.01MPa, and cool the cooked medium to 25°C for use;
[0044] The solid medium is composed of A component and B component, and the A component is composed of the following components by mass percentage:
[0045]
[0046] The B component is underground seawater, and the mass ratio of the B component to the A component is 4:1; the preparation method of the solid medium: weigh the A component and the B component in the above mass percentage, and A component is added into underground seawater with a salinity of 32wt‰ and stirred evenly to obtain a solid medium. Prepare a solid medium. The underground seawater is filtered with a double-layer 250-mesh cotton cloth, and the stirring rate ...
Embodiment 3
[0052] The difference between embodiment 3 and embodiment 1 is:
[0053] A kind of cultivation method of spirulina of the present invention comprises the steps:
[0054] (1) Place the solid medium in a sterilizing pot and cook for 95 minutes, keep the temperature at 124°C and the air pressure at 0.02MPa, and cool the cooked medium to 30°C for use;
[0055] The solid medium is composed of A component and B component, and the A component is composed of the following components by mass percentage:
[0056]
[0057] The B component is underground seawater, and the mass ratio of the B component to the A component is 3:1; the preparation method of the solid medium: weigh the A component and the B component in the above mass percentage, and A component is added into underground seawater with a salinity of 35wt‰ and stirred evenly to obtain a solid medium. The quality of the underground seawater accounts for 80% of the total mass of the components. The underground seawater is fil...
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