Direct fluorescence spectrum detection method for content of cystine in cystine tablet

Abstract

The invention discloses a direct fluorescence spectrum detection method for the content of cystine in a cystine tablet. The content of the cystine tablet is directly measured by adopting a sensitive fluorescence spectrophotometer. Experiments show that cystine molecules show specific luminescent properties in an alkaline solution environment, and the content of the cystine can be directly measured by using a good linear relationship between the content of the cystine and fluorescence intensity under certain conditions. The direct fluorescence spectrum detection method disclosed by the invention has the advantages of high precision, good accuracy, mild reaction conditions and lower cost of a used instrument; a complex pretreatment process is not needed, an operation process is simple, and time and labor are saved; a used solvent is simple and easily-obtained, and has less injury to an operator and less pollution.

Description

technical field [0001] The invention relates to a spectrum detection method, in particular to a direct fluorescence spectrum detection method for cystine content in cystine tablets. Background technique [0002] Cystine (Cystine, Cys) (C6H12N2O4S2, Mr=240.30) is an amino acid obtained by oxidation of two molecules of cysteine. It is a sulfur-containing amino acid and belongs to the class of disulfides. -alanine)”; is the basic unit of protein, widely exists in hair, bone, keratin, the content in human hair is about 5% (by mass), and the normal value in human body is 4.40~11.52μmol / L. [0003] As a nutritional enhancer in medicine, cystine can promote the oxidation and reduction functions of body cells, improve liver function, promote white blood cell proliferation, etc., and can neutralize toxins and prevent the growth of pathogenic bacteria; due to the important role of cystine in physiological research It is a widely used amino acid drug; cystine tablets are recorded in...

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Problems solved by technology

[0003] As a nutritional enhancer in medicine, cystine can promote the oxidation and reduction functions of body cells, improve liver function, promote white blood cell proliferation, etc., and can neutralize toxins and prevent the growth of pathogenic bacteria; due to the important role of cystine in physiological research It is a widely used amino acid drug; cystine tablets are recorded in the Pharmacopoeia of the People's Republic of China (2015 Edition) (Part Two) (Chemical Drugs 1896), and the traditional potassium bromate titration colorimetric method is used. It is cumbersome and has large errors; with the development of technology, there are many researches on related instrumental analysis methods, currently reported are atomic emission spectrometry (ICP-AES), amino acid analyzer assay (AA), high performance liquid chromatography (HPLC) , Spectrophotometry (UV), High Performance Capillary Electrophoresis (HPCE), Electrochemical (EC), Flow Injection Fluorescence (FI-FL), High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS), etc.; these methods It is often interfered by coexisting amino acids, and has poor selectivity for non-single cystine systems; electrochemical analysis methods usually use different types of amino acid selective electrodes to measure various amino acids; when cystine is used for oxidation reactions on electrodes The electrical activity presented can be used without derivatization treatment, and its content can be indirectly measured by using the coordination reaction between cystine and metal ions, but there is a problem of large interference; high performance liquid chromatography is especially effective for pre-column derivatization-reaction Phase-high performance liquid chromatography (RP-HPLC) analysis method is more sensitive (can be detected at <1pmol level) and fast (it only takes 12-30min to complete the hydrolysis, separation and determination of a protein); but RP-HPLC requires the amino acid It is converted into derivatives that are suitable for reversed-phase chromatographic separation and can be sensitively detected before the column. Therefore, there are problems such as expensive instruments and equipment, difficult selection of derivatization reagents, long derivatization time and complex components of derivatization products; other instrumental analysis All methods need to derivatize cystine with a revealing agent and then measure it indirectly. There are problems such as long derivatization time, complex components of derivatized products, and poor stability.

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