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Preparation method of composite microbial inoculum for producing silage from rotten vegetable leaves and straw

A technology of silage and compound bacterial agent, which is applied in the direction of animal feed, animal feed, application, etc., can solve the problems of difficult control of feed quality, inability to effectively reduce acidity, and single bacterial species, so as to improve the quality of livestock meat and milk, and promote sustainable development. Continuous development, avoiding the effect of secondary fermentation

Active Publication Date: 2017-05-17
NORTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The patent with the publication number CN103518957A uses tail vegetables and wheat straw to produce silage, and the added strain is single, which cannot effectively reduce the acidity in a short period of time, and the quality of the feed is difficult to control
At present, there is still a lack of commercial bacteria agents for the production of silage from tail vegetables and straw

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 is used for the preparation method of the compound bacterial agent of tail vegetables and straw production silage, comprises the following steps:

[0023] (1) Mix 10kg milk and 1kg brown sugar evenly to obtain solution A.

[0024] Among them: milk refers to skimmed milk after pasteurization.

[0025] (2) Add 0.2kg of lactic acid bacteria mixture to 10kg of solution A, mix well, then add 1 part of inorganic salt per 1L of mixture to obtain solution B.

[0026] Wherein: 1 part of inorganic salt is composed of 2.0 g of disodium citrate, 2.0 g of potassium dihydrogen phosphate, 2.0 g of dipotassium hydrogen phosphate, 0.05 g of manganese sulfate, and 20.0 g of calcium carbonate.

[0027] The lactic acid bacteria mixture is prepared as follows:

[0028] ①Dilute Lactobacillus plantarum (CICC6026), Pediococcus lactis (CGMCC1.2696), Enterococcus faecalis (CICC20440), and Bifidobacterium animalis (CICC 21717) with 10 mL of sterile water, and then take 1 mL of the ...

Embodiment 2

[0033] Embodiment 2 The preparation method of the compound bacterial agent that is used for tail vegetables and stalks to produce silage comprises the following steps:

[0034] (1) Mix 20kg milk and 1kg brown sugar evenly to obtain solution A.

[0035] Wherein milk is with embodiment 1.

[0036] (2) Add 0.4kg of lactic acid bacteria mixture to 20kg of solution A, mix well, then add 1 part of inorganic salt per 1L of mixture to obtain solution B.

[0037] Wherein: 1 part of inorganic salt, lactic acid bacteria mixed solution are the same as embodiment 1.

[0038] (3) Add 12 kg of corn flour with a fineness of 80-100 mesh to 2 kg of solution B, and stir evenly to obtain a raw material with a moisture content of 30%-45%.

[0039] ⑷Put raw materials into anaerobic bags, ferment at 28°C~36°C for 3~5 days, when the pH value reaches 3.8~4.1, and the lactic acid bacteria reach 20~20 billion cfu / g, dry and pulverize under aseptic conditions , Packed into bags to obtain the special b...

Embodiment 3

[0040] Embodiment 3 The preparation method of the compound microbial agent that is used for tail vegetables and stalks to produce silage comprises the following steps:

[0041] (1) Mix 15kg milk and 1kg brown sugar evenly to obtain solution A.

[0042] Wherein milk is with embodiment 1.

[0043] (2) Add 0.3kg of lactic acid bacteria mixture to 15kg of solution A, mix well, add 1 part of inorganic salt per 1L of mixture to obtain solution B.

[0044] Wherein: 1 part of inorganic salt, lactic acid bacteria mixed solution are the same as embodiment 1.

[0045] (3) Add 11 kg of corn flour with a fineness of 80 to 100 mesh to 1.5 kg of solution B, and stir evenly to obtain raw materials with a moisture content of 30% to 45%.

[0046] ⑷Put raw materials into anaerobic bags, ferment at 28°C~36°C for 3~5 days, when the pH value reaches 3.8~4.1, and the lactic acid bacteria reach 20~20 billion cfu / g, dry and pulverize under aseptic conditions , Packed into bags to obtain the special...

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Abstract

The invention relates to a preparation method of composite microbial inoculum for producing silage from rotten vegetable leaves and straw. The method comprises the following steps of (1) uniformly mixing milk with brown sugar in proportion so as to obtain a solution A; (2) adding lactic acid bacteria mixed liquor to the solution A, performing uniform mixing to obtain mixed liquor, and adding inorganic salts to the mixed liquor in a manner of adding 1 part of the inorganic salts to each liter of the mixed liquor so as to obtain a solution B; (3) adding corn flour to the solution B, and performing uniform stirring so as to obtain raw materials of which the moisture content is 30-45%; and (4) loading the raw materials into anaerobic bags, performing fermentation under the condition of 28-36 DEG C for 3-5 days, when the pH value reaches 3.8-4.1 and lactic acid bacteria reach 2-20 billion cfu / g, performing drying under an aseptic condition, performing crushing, and performing bagging so as to obtain the microbial inoculum special for the silage. The preparation method disclosed by the invention is simple in technology and low in cost; and through the obtained composite microbial inoculum, the fermentation time of the silage is shortened, and the fermentation efficiency is improved.

Description

technical field [0001] The invention relates to the field of silage production, in particular to a preparation method of a compound bacterial agent used for producing silage from tail vegetables and straw. Background technique [0002] In recent years, the vegetable industry in Gansu has developed rapidly, and it has become one of the five major commercial vegetable bases in the country's "West-to-East Vegetable Transfer" and "North-to-South Vegetable Transport" and a key production area for exporting vegetables in the northwest inland. Especially in the main vegetable producing areas such as the Yellow River Irrigation Area, Yuzhong Basin, Hexi Corridor, and Huicheng Basin, taking advantage of the climate characteristics of cool summer temperature, sufficient sunshine, and large temperature difference between day and night on the Northwest Plateau, the vegetables produced have a longer harvesting and marketing period. It makes up for the gap in the supply of summer vegetabl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12
Inventor 臧荣鑫徐红伟杨具田任瑞杨研梅牛荇洲
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
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