Preparation method of composite microbial inoculum for producing silage from rotten vegetable leaves and straw
A technology of silage and compound bacterial agent, which is applied in the direction of animal feed, animal feed, application, etc., can solve the problems of difficult control of feed quality, inability to effectively reduce acidity, and single bacterial species, so as to improve the quality of livestock meat and milk, and promote sustainable development. Continuous development, avoiding the effect of secondary fermentation
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Embodiment 1
[0022] Embodiment 1 is used for the preparation method of the compound bacterial agent of tail vegetables and straw production silage, comprises the following steps:
[0023] (1) Mix 10kg milk and 1kg brown sugar evenly to obtain solution A.
[0024] Among them: milk refers to skimmed milk after pasteurization.
[0025] (2) Add 0.2kg of lactic acid bacteria mixture to 10kg of solution A, mix well, then add 1 part of inorganic salt per 1L of mixture to obtain solution B.
[0026] Wherein: 1 part of inorganic salt is composed of 2.0 g of disodium citrate, 2.0 g of potassium dihydrogen phosphate, 2.0 g of dipotassium hydrogen phosphate, 0.05 g of manganese sulfate, and 20.0 g of calcium carbonate.
[0027] The lactic acid bacteria mixture is prepared as follows:
[0028] ①Dilute Lactobacillus plantarum (CICC6026), Pediococcus lactis (CGMCC1.2696), Enterococcus faecalis (CICC20440), and Bifidobacterium animalis (CICC 21717) with 10 mL of sterile water, and then take 1 mL of the ...
Embodiment 2
[0033] Embodiment 2 The preparation method of the compound bacterial agent that is used for tail vegetables and stalks to produce silage comprises the following steps:
[0034] (1) Mix 20kg milk and 1kg brown sugar evenly to obtain solution A.
[0035] Wherein milk is with embodiment 1.
[0036] (2) Add 0.4kg of lactic acid bacteria mixture to 20kg of solution A, mix well, then add 1 part of inorganic salt per 1L of mixture to obtain solution B.
[0037] Wherein: 1 part of inorganic salt, lactic acid bacteria mixed solution are the same as embodiment 1.
[0038] (3) Add 12 kg of corn flour with a fineness of 80-100 mesh to 2 kg of solution B, and stir evenly to obtain a raw material with a moisture content of 30%-45%.
[0039] ⑷Put raw materials into anaerobic bags, ferment at 28°C~36°C for 3~5 days, when the pH value reaches 3.8~4.1, and the lactic acid bacteria reach 20~20 billion cfu / g, dry and pulverize under aseptic conditions , Packed into bags to obtain the special b...
Embodiment 3
[0040] Embodiment 3 The preparation method of the compound microbial agent that is used for tail vegetables and stalks to produce silage comprises the following steps:
[0041] (1) Mix 15kg milk and 1kg brown sugar evenly to obtain solution A.
[0042] Wherein milk is with embodiment 1.
[0043] (2) Add 0.3kg of lactic acid bacteria mixture to 15kg of solution A, mix well, add 1 part of inorganic salt per 1L of mixture to obtain solution B.
[0044] Wherein: 1 part of inorganic salt, lactic acid bacteria mixed solution are the same as embodiment 1.
[0045] (3) Add 11 kg of corn flour with a fineness of 80 to 100 mesh to 1.5 kg of solution B, and stir evenly to obtain raw materials with a moisture content of 30% to 45%.
[0046] ⑷Put raw materials into anaerobic bags, ferment at 28°C~36°C for 3~5 days, when the pH value reaches 3.8~4.1, and the lactic acid bacteria reach 20~20 billion cfu / g, dry and pulverize under aseptic conditions , Packed into bags to obtain the special...
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