High-temperature resistant xylanase containing feed additive and application thereof
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A feed additive and xylanase technology, applied in the field of feed additives, can solve the problems of high temperature resistance, xylanase inactivation, etc., and achieve the effects of reducing the viscosity of chyme, reducing the rate of diarrhea, and saving crude protein
Inactive Publication Date: 2017-05-17
ANHUI LEVEKING BIOTECH CO LTD
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Problems solved by technology
[0004] Existing literature discloses a lot of xylanases used as feed additives, but most of them are not resistant to high temperature, so when adding feed additives containing xylanase to make feed, the high temperature in the process of making feed instantly makes xylanase Irreversible inactivation of enzyme preparations such as carbohydrases
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Embodiment 1
[0034] Embodiment 1: the preparation of thermostable xylanase xyn-LVK
[0035] 1. Experimental conditions
[0036] (1) Strains and vectors
[0037] Escherichia coli E. coli Top10 was purchased from Invitrogen, Trichoderma reesei QM9414 was purchased from American Type Culture Collection (ATCC), and pUC19 and pMD19-T simple vector were purchased from TaKaRa. Plasmid pAN7-1 (with fungal selection marker hygromycin resistance gene hph and E. coli selection marker ampicillin resistance gene Ap).
[0038] (2) Enzymes and other biochemical reagents
[0039] Various DNA tool enzymes, DNA Marker, DNA Gel Recovery and Purification Kit (Agarose Gel DNA Purification Kit), DNA Purification Kit (Fragment Purification Kit Ver. 2.0) were purchased from TaKaRa. T4 DNA ligase was purchased from Fermetas. Other biochemical reagents were purchased from Shanghai Sangon Bioengineering Technology Service Co., Ltd.
[0040] (3) Medium
[0041] LB medium: for cultivating Escherichia coli, conta...
Embodiment 2
[0076] The formula of the feed additive is as follows (mass fraction): xylanase xyn-LVK 10%, dextranase 15%, cellulase 1.5%, mannanase 0.75%, protease 20%, (soybean meal + DDGS) 52.75 %.
[0077] The enzyme activity of xylanase xyn-LVK in the feed additive is 10000 u / g, the enzyme activity of dextranase is 3000 u / g, the enzyme activity of cellulase is 300 u / g, and the activity of mannanase The enzyme activity is 300 u / g, and the enzyme activity of protease is 20000 u / g.
Embodiment 3
[0079] The formula of the feed additive was as follows (mass fraction): xylanase xyn-LVK 12%, glucanase 13%, cellulase 2.5%, mannanase 1%, protease 18%, DDGS 53.5%.
[0080] The enzyme activity of xylanase xyn-LVK in the feed additive is 12000 u / g, the enzyme activity of dextranase is 2600 u / g, the enzyme activity of cellulase is 500 u / g, and the activity of mannanase The enzyme activity is 400 u / g, and the enzyme activity of protease is 18000 u / g.
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Abstract
The invention discloses a high-temperature resistant xylanase containing feed additive and application thereof. The high-temperature resistant xylanase containing feed additive consists of a compound enzyme and a carrier, wherein the compound enzyme is mainly high-temperature resistant xylanase; the most appropriate xylanase degradation temperature of the high-temperature resistant xylanase is 75 DEG C; 42-65% of xylanase degradation activity can be still maintained when the high-temperature resistant xylanase is treated for 4-8 minutes at 90 DEG C. According to the high-temperature resistant xylanase containing feed additive disclosed by the invention, soluble and insoluble non-starch polysaccharides antinutritional factors in fiber daily ration can be effectively degraded by using the compound enzyme which is mainly high-temperature resistant xylanase, so that cell walls can be broken, the opportunity that endogenous enzymes secreted from animals contact corresponding substrates can be increased, meanwhile the soybean protein decomposition rate can be remarkably increased due to protease in the compound enzyme, the antinutritional factors can be degraded, diarrhea caused by insufficient protein digestion of young animals with insufficient endogenous enzyme secretion can be also avoided, and furthermore the digestion rate of protein raw materials such as miscellaneous meal which is not easy to digest can be directly increased.
Description
technical field [0001] The invention relates to the technical field of feed additives, in particular to a feed additive containing high temperature resistant xylanase and its application in improving animal body weight and / or feed conversion rate. Background technique [0002] Due to the serious shortage of feed materials such as corn and soybean meal, in order to meet the rapidly developing feed demand, it is objectively required to make full use of my country's rich resources of wheat bran, rice bran, rapeseed meal, cotton meal, etc. The anti-nutritional factors in these vegetable raw materials are mainly non-starch polysaccharides, especially soluble non-starch polysaccharides. In order to effectively utilize these feed ingredients, it is necessary to use enzyme preparations such as xylanase at the same time. At the same time, as my country's food safety is increasingly valued, the use of antibiotics and other feed additives that are prone to residues and pollute the env...
Claims
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