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Animal feed containing high-temperature-resisting xylanase and application of animal feed

A xylanase and animal feed technology, applied in animal feed, animal feed, applications, etc., can solve the problems of high temperature resistance, xylanase inactivation, etc., to increase animal weight, reduce diarrhea rate, and improve feed conversion rate effect

Inactive Publication Date: 2017-05-17
ANHUI LEVEKING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing literature discloses a lot of xylanases used as feed additives, but most of them are not resistant to high temperature. Therefore, when adding xylanase to the feed, the high temperature instantly makes the xylanase irreversible in the process of making the feed. Inactivate

Method used

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  • Animal feed containing high-temperature-resisting xylanase and application of animal feed
  • Animal feed containing high-temperature-resisting xylanase and application of animal feed
  • Animal feed containing high-temperature-resisting xylanase and application of animal feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the preparation of thermostable xylanase xyn-LVK

[0038] 1. Experimental conditions

[0039] (1) Strains and vectors

[0040] Escherichia coli E. coli Top10 was purchased from Invitrogen, Trichoderma reesei QM9414 was purchased from American Type Culture Collection (ATCC), and pUC19 and pMD19-T simple vector were purchased from TaKaRa. Plasmid pAN7-1 (with fungal selection marker hygromycin resistance gene hph and E. coli selection marker ampicillin resistance gene Ap).

[0041] (2) Enzymes and other biochemical reagents

[0042] Various DNA tool enzymes, DNA Marker, DNA Gel Recovery and Purification Kit (Agarose Gel DNA Purification Kit), DNA Purification Kit (Fragment Purification Kit Ver. 2.0) were purchased from TaKaRa. T4 DNA ligase was purchased from Fermetas. Other biochemical reagents were purchased from Shanghai Sangon Bioengineering Technology Service Co., Ltd.

[0043] (3) Medium

[0044] LB medium: for cultivating Escherichia coli, conta...

Embodiment 2

[0078] Example 2 The effect of the animal feed containing thermostable xylanase of the present invention on the growth performance of yellow-feathered broiler chickens.

[0079] Test time: July-August 2014;

[0080] Test location: a large feed enterprise in Guangdong;

[0081] Experimental design: 800 1-day-old yellow-feathered broiler chickens were selected and divided into 4 treatments, namely positive control group, test I group, negative control group and test II group. Each treatment has 4 repetitions, 50 rats per repetition, and the formal test period is 42 days.

[0082]

[0083] The basic diet consists of the following components by mass fraction: corn 66.50%, soybean meal: 19.50%, corn gluten meal 7.10%, oil 3.00%, calcium hydrogen phosphate 0.37%, stone powder 1.47%, lysine 0.57%, methionine 0.22% , salt 0.17%, choline 0.10%, premix 1%. The premix contains trace elements, vitamins, digestive enzymes and acidulants. The basal diet was reduced by 50kcal / kg by ad...

Embodiment 3

[0088] Example 3 The effect of the animal feed containing thermostable xylanase of the present invention on the growth performance of suckling piglets.

[0089] Test time: September-October 2014;

[0090] Test location: a large pig farm in Guangdong;

[0091] Experimental design: 150 weaned piglets with similar body weight at 21 days of age were selected and randomly divided into 2 groups, with 5 repetitions in each group and 15 piglets in each repetition. The formal experiment period was 28 days.

[0092]

[0093] The basic diet consisted of the following components by mass fraction: corn 70.25%, soybean meal 22.15%, fish meal 5.0%, calcium hydrogen phosphate 1%, stone powder 0.35%, salt 0.25%, and premix 1%. The premix contains trace elements, vitamins, digestive enzymes and acidulants.

[0094] Measuring indicators: daily weight gain; daily feed intake; feed-to-meat ratio; diarrhea rate.

[0095] test results:

[0096]

[0097] It can be seen from the above table ...

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Abstract

The invention discloses animal feed containing high-temperature-resisting xylanase. The animal feed is prepared from basic ration; the optimum temperature for degrading xylan by the high-temperature-resisting xylanase is 75DEG C; in addition, after the high-temperature-resisting xylanase is treated at the temperature of 90DEG C for 4 to 8 minutes, 42 to 65 percent of xylan degradation activity also can be retained. The animal feed containing the high-temperature-resisting xylanase, disclosed by the invention, is applied to improvement on body weight of an animal and / or conversion rate of feed, so that the slaughter weight of broiler chicken is increased by 2.33 to 3.49 percent, and the feed-to-meat ratio is reduced by 1.73 to 4.49 percent; daily grain and daily food consumption of weanling pigs can be increased, the conversion rate of the feed can be improved, and the diarrhea rate can be reduced.

Description

technical field [0001] The invention relates to the technical field of animal feed, in particular to an animal feed containing high-temperature resistant xylanase and its application in improving animal body weight and / or feed conversion rate. Background technique [0002] Due to the serious shortage of feed materials such as corn and soybean meal, in order to meet the rapidly developing feed demand, it is objectively required to make full use of my country's rich resources of wheat bran, rice bran, rapeseed meal, cotton meal, etc. The anti-nutritional factors in these vegetable raw materials are mainly non-starch polysaccharides, especially soluble non-starch polysaccharides. In order to effectively utilize these feed ingredients, it is necessary to use enzyme preparations such as xylanase at the same time. At the same time, as my country's food safety is increasingly valued, the use of antibiotics and other feed additives that are prone to residues and pollute the environ...

Claims

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Application Information

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IPC IPC(8): A23K50/75A23K50/60A23K10/30A23K10/37A23K20/189A23K20/142A23K20/174A23K10/18
CPCY02P60/87
Inventor 吴广兵王剑英郭宏涛林智谭福建
Owner ANHUI LEVEKING BIOTECH CO LTD
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