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Method for purifying polyphenol substance system

A purification method and technology for polyphenols, which are applied in the field of system purification of polyphenols, can solve problems such as no reports on research, and achieve the effects of less energy consumption, reduced pollution and high efficiency

Inactive Publication Date: 2017-05-24
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few studies on mango peel in my country. There are domestic researchers who extract pectin, pigments and feed from mango peel, but there is no report on the polyphenols of mango peel.

Method used

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  • Method for purifying polyphenol substance system
  • Method for purifying polyphenol substance system
  • Method for purifying polyphenol substance system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A systemic purification method for polyphenols, the specific steps are:

[0022] 1) Chopped and blanched; weigh 100g of mango skin, chop it, and blanch in boiling water for 0.5min;

[0023] 2) Inactivation of polyphenol oxidase: Sodium sulfite mixed with vitamin C, soaked in 0.5% sodium sulfite for 1 min, then directly added 0.5% vitamin C;

[0024] 3) Crushing and homogenization: before crushing, rinse the mango skin with running water, and immediately add ultra-pure water according to the ratio of 1:5 material to liquid and crush it with a beater;

[0025] 4) Ethanol leaching: According to the volume of the crushed slurry, add absolute ethanol to the slurry according to a certain volume ratio, and place the mixed solution in a 60°C water bath for 1.0h;

[0026] 5) Centrifugation: Combine the filtrates, then centrifuge them at a rate of 5000r / min for 5min and take the supernatant after centrifugation;

[0027] 6) Recovery of the purified product: recovery of ethanol ...

Embodiment 2

[0030] A systemic purification method for polyphenols, the specific steps are:

[0031] 1) Chopped and blanched; weigh 100g of mango skin, chop it, and blanch in boiling water for 0.5min;

[0032] 2) Inactivation of polyphenol oxidase: Sodium sulfite mixed with vitamin C, soaked in 0.5% sodium sulfite for 1 min, then directly added 0.5% vitamin C;

[0033] 3) Crushing and homogenization: before crushing, rinse the mango skin with running water, and immediately add ultra-pure water according to the ratio of 1:5 material to liquid and crush it with a beater;

[0034] 4) Ethanol extraction: according to the volume of the crushed slurry, add absolute ethanol to the slurry according to the volume ratio of 2:3, and place the mixed solution in a water bath box at a certain temperature for extraction for 1.0 h;

[0035] 5) Centrifugation: Combine the filtrates, then centrifuge them at a rate of 5000r / min for 5min and take the supernatant after centrifugation;

[0036] 6) Recovery of...

Embodiment 3

[0039] A systemic purification method for polyphenols, the specific steps are:

[0040] 1) Chopped and blanched; weigh 100g of mango skin, chop it, and blanch in boiling water for 0.5min;

[0041] 2) Inactivation of polyphenol oxidase: Sodium sulfite mixed with vitamin C, soaked in 0.5% sodium sulfite for 1 min, then directly added 0.5% vitamin C;

[0042] 3) Crushing and homogenization: before crushing, rinse the mango skin with running water, and immediately add ultra-pure water according to the ratio of 1:5 material to liquid and crush it with a beater;

[0043] 4) Ethanol extraction: according to the volume of the crushed slurry, add absolute ethanol to the slurry according to the volume ratio of 2:3, and place the mixed solution in a 60°C water bath for a certain period of time;

[0044] 5) Centrifugation: Combine the filtrates, then centrifuge them at a rate of 5000r / min for 5min and take the supernatant after centrifugation;

[0045] 6) Recovery of the purified produc...

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Abstract

The invention discloses a method for purifying a polyphenol substance system. The method comprises a step 1) of performing cutting up and hot iron; a step 2) of inactivating polyphenol oxidase; a step 3) of performing crushing and homogenizing; a step 4) of performing alcohol extraction; a step 5) of performing centrifugation and a step 6) of recycling a purified substance. According to the method, waste mango skin is taken as a raw material, polyphenol substances are extracted from the waste mango skin, mango skin polyphenols are water-soluble substances, and the water solubility of the mango skin polyphenols increases with the rise of the temperature. The mango skin contains substances such as pectin, polysaccharide and protein and the like, a certain concentration of alcohol solution has a certain blocking effect on the polyphenols and hydrogen bonds and hydrophobic bonds formed by mutual association of the polyphenols, alcohol has high polyphenol solubility and is low in protein and polysaccharide solubility, and pectin is not dissolved to alcohol. Compared with a conventional purification technology, the method has the advantages of being simple and convenient to operate, quick in purification speed, high in efficiency and low in energy consumption and the like, and product pollution and thermosensitive substance decomposition can further be reduced.

Description

technical field [0001] The invention relates to a method for systematically purifying polyphenols, which belongs to the technical field of food processing. Background technique [0002] Mango is a perennial plant of the cactaceae Anacardiaceae family. Mango is also known as "Wangguo", which means "fruit of hope". It is rich in nutrition, so it is also known as the "king of tropical fruits". Mango skin accounts for about 10% of the weight of mango fruit. It is rich in carbohydrates, protein, and fat needed by the human body, and is also rich in vitamins A, B, and C. Wherein the content of vitamin A is as high as 3.8%. Mango skin also contains calcium, phosphorus, iron and other mineral elements necessary for the human body. In addition, mango peel also has high medicinal value. "Food Herbs" records: "Mango can cure the disease of 'the woman's meridian is blocked, and the husband's blood in the camp is not good'." Features. [0003] The annual output of mango skin is la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L19/00A23L33/10
CPCA23V2002/00A23V2250/082A23V2250/21A23V2250/2132
Inventor 张新李武
Owner GUANGXI UNIV