Sunscreen repairing cosmetic composition and its preparation method and application
A cosmetic composition and extract technology, applied in cosmetic preparations, cosmetics, cosmetic preparations and other directions, can solve problems such as inability to reduce skin damage, and achieve the effects of preventing melanin deposition, maintaining skin elasticity, and resisting photoaging
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[0055] Further preferably, the preparation method further includes the step of: filtering the liquid coralline algae extract and vacuum drying to obtain a solid coralline algae extract.
[0056] Specifically, after grinding the coralline algae degreased by ethanol, take an appropriate amount and place it in a soaking tank, and add a propylene glycol aqueous solution, wherein the solid-liquid ratio of the coralline algae to the propylene glycol aqueous solution is 1:5-1:60, and the propylene glycol In the aqueous solution, the mass ratio of water to propylene glycol is 2-1:1-4, soaked for 4-20 hours (proper stirring can be carried out during the soaking process), and the soaking solution is obtained; then the soaking solution is transferred to the extraction tank for microwave extraction, The time of microwave extraction is 5-40 minutes, the temperature of microwave extraction is 20-100°C, the power of microwave extraction is 300-1500W, and the liquid coralline algae extract is ...
Embodiment 1-31
[0121] The preparation method of coralline algae extract is:
[0122] Step 1), soaking the pulverized coralline algae in an aqueous propylene glycol solution to obtain a soaking solution;
[0123] In step 2), microwave extraction is performed on the soaking liquid, and coralline algae extracts are obtained according to the different preparation conditions shown in Table 1. Then the protective effect of the obtained coralline algae extract on keratinocytes was measured, and the results are shown in Table 1.
[0124] Wherein, the test method of keratinocyte survival rate: adopt MTT method to measure cell viability. Keratinocytes were divided into 5×10 3 Inoculated in 96-well plate at a density of / mL, and cultured until the cells were 60-70% confluent, they were divided into two groups, A and B. The cells in group A were conventionally cultured for 24 hours after being irradiated by infrared rays, and the cells in group B were added with coralline algae extracts obtained under d...
Embodiment 32-62
[0129] The preparation method of watermelon fruit extract is:
[0130] Step 1), soaking the ground watermelon fruit in an aqueous propylene glycol solution to obtain a soaking solution;
[0131] Step 2), ultrasonically extracting the soaking solution to obtain a crude extract;
[0132] Step 3), after adding ethanol in the crude extract, let it stand, then remove the ethanol in the crude extract, obtain the watermelon fruit extract according to the different preparation conditions shown in Table 2, and then measure the obtained watermelon The protective effect of fruit extract on DNA in cells is shown in Table 2 below.
[0133] Test method for DNA protection: culture fibroblasts in vitro, add watermelon fruit extract to culture for 2 hours and then irradiate with ultraviolet light, then use single-cell gel electrophoresis (i.e. comet test) to detect the protection of the extract on DNA in the cells Happening.
[0134] The specific detection steps are:
[0135] Cell preparat...
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