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Cytoplasm sterile hybrid seed production method for cabbage type rape in Guanzhong region

A cabbage rape and hybrid seed production technology, which is applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problem of low seed production yield, inability to meet mass production, low hybrid rape seed production purity, and high seed production cost. It can reduce the outcrossing seed setting rate, eliminate the problem of low temperature micropowder self-crossing, and increase the yield of seed production.

Inactive Publication Date: 2017-05-31
咸阳市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main factors affecting the low purity of hybrid rape seed production are: (1) the influence of micro pollen; (2) the influence of the surrounding environment; (3) the low purity of the parents; (4) various mechanical mixing in the process of seed production and seed processing, etc.
In the existing rapeseed seed production methods, two-line recessive sterility hybridization is mostly used, but the seed production of this method is relatively difficult, the cost of seed production is high, and the yield of seed production is low, which cannot meet the needs of mass production

Method used

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  • Cytoplasm sterile hybrid seed production method for cabbage type rape in Guanzhong region

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] On September 15th, the seed production field was prepared, and 15kg of urea, 30kg of calcium phosphate, 10kg of potassium chloride and 0.5kg of boron fertilizer were applied per mu; and small and shallow trenches were opened, and after sowing, the ditch was covered with 2cm deep. Soil, spread 1kg of carbofuran granules along the ditch per mu of land; and before seed production and sowing, set up a safety isolation area around the seed field, and the distance between the outer edge of the safety isolation area and the seed field is 2000m.

[0029] Use 25g of carbendazim per kilogram of rapeseed for seed dressing, and put the finished seeds into pre-marked plastic bottles according to the restorer line as the male parent and the male sterile line as the female parent. The row ratio of the row to the female parent row is 2:1, and the two sides of each female parent row are guaranteed to be one parent row respectively, and two parent rows are planted between every two adjace...

Embodiment 2

[0038] On September 16th, the seed field was prepared, and 15kg of urea, 10kg of potassium chloride and 0.5kg of boron fertilizer were applied per mu; small trenches and shallow trenches were opened, and 2cm deep soil was covered along the trench after sowing. Spread 1kg of carbofuran granules in the ditch; and before seed production and sowing, set up a safety isolation area around the seed field, and the distance between the outer edge of the safety isolation area and the seed field is 2050m.

[0039] Each kilogram of rapeseed seeds was soaked and dressed with 10 g of 25% Rheumatoidin, and after the seeds were air-dried, they were loaded into the restorer line as the male parent and the male sterile line as the female parent. In the plastic bottles marked in advance, sow seeds according to the row ratio of the male parent row and the female parent row 2:1, and ensure that there is a male parent row on both sides of each female parent row, and every two adjacent female parent ...

Embodiment 3

[0048] On September 17th, the seed production field was prepared with 15kg urea; and small ditches and shallow ditches were opened, after sowing, the ditch was covered with 2cm-deep soil, and 1kg of carbofuran granules were spread along the ditch per mu of land; Before seed production and sowing, a safety isolation area shall be set up around the seed field, and the distance between the outer edge of the safety isolation area and the seed field shall be 2100m.

[0049] Use 25g of carbendazim per kilogram of rapeseed for seed dressing, and put the finished seeds into pre-marked plastic bottles according to the restorer line as the male parent and the male sterile line as the female parent. The row ratio of the row to the female parent row is 2:1, and the two sides of each female parent row are guaranteed to be one father parent row, and two parent parent rows are planted between every two adjacent female parent rows. The sowing rate of the male parent row and each female parent...

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Abstract

The invention relates to a cytoplasm sterile hybrid seed production method for cabbage type rape in the Guanzhong region, and belongs to the field of hybrid seed production. The method comprises the following steps: firstly, selecting a restorer line as a male parent and a male sterile line as a female parent; pretreating the male parent and the female parent respectively, and sowing the male parent and the female parent after pretreatment at an interval in a seed production field provided with a safe isolation area to form a male parent row and a female parent row; spraying a chemical male gametocide to kill stamens when the main inflorescence diameter of the female parent is 6-12mm and the stalk height is 10-20cm; and performing field management until hybrid seeds are harvested. The chemical male gametocide is prepared according to the following steps: taking 100% tribenuron-methyl to prepare a mother solution according to the concentration of 0.8-1.2g / L, and mixing the mother solution and a detergent with water to obtain the chemical male gametocide, wherein the volume percentage of the mother solution in the chemical male gametocide is 0.03-0.05%. The method can promote the emergence rates of the male and female parents for seed production and improve the seed production output, the prepared hybrid seeds are high in purity and cleanliness, and the economic benefit of hybrid seed production is increased.

Description

technical field [0001] The invention relates to the field of hybrid seed production, in particular to a cytoplasmic sterile hybrid seed production method for Brassica napus in Guanzhong area. Background technique [0002] The utilization of heterosis is an important way to improve crop yield. There are many ways to utilize heterosis in rapeseed, mainly including cytoplasmic male sterility, nuclear male sterility, chemical male sterility and so on. The heterosis of rapeseed is remarkable, generally can increase the yield by 20%-30%, and the good combination can increase the yield by 50%. The popularization and application of hybrid rapeseed is developing rapidly. Only the area of ​​hybrid rapeseed in my country accounts for more than 70% of the total area of ​​rapeseed. [0003] At present, the main problem facing the development of hybrid rapeseed is the problem of seed purity, which is also the key to affecting the potential of hybrid rapeseed to increase yield. According...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 薛汉军贾战通苟建鹏俱苏耀常建军华哲陈宏
Owner 咸阳市农业科学研究院
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