Magnolia liliiflora tissue culture process

Abstract

The invention discloses a magnolia liliiflora tissue culture process. The magnolia liliiflora tissue culture process includes following steps: S1, performing sterilization and bacteriostasis treatment on a culture site of magnolia liliiflora tissue; S2, selecting magnolia liliiflora branches 1-3cm in diameter and with axillary buds from maternal plants of magnolia liliiflora, which grow strongly and are free from plant diseases and insect pests, as explant; S3, first using tap water for washing the explant for 10-12min, then using a mercury chloride and polysorbate mixture 0.1-0.3% in concentration for steeping the explant for 8-12min, and finally using sterile water for washing the explant for 4-7min; S4, preparing raw materials by following weight part: 20-25 parts of alcohol, 21-24 parts of indole-3-butyric acid potassium, 15-20 parts of talcum powder, 12-16 parts of clay, 25-30 parts of naphthylacetic sodium salt, 18-22 parts of plant ash and 17-21 parts of volcanic ash. The magnolia liliiflora tissue culture process can ensure good quality of the magnolia liliiflora after tissue culture, can substantially improve rooting rate and survival rate of the magnolia liliiflora in the tissue culture, can further improve the quality of the magnolia liliiflora after the tissue culture, and is simple in technology and convenient to use.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a magnolia tissue culture process. Background technique [0002] Purple magnolia, also known as magnolia and magnolia, is a unique plant in China. It is distributed in Yunnan, Fujian, Hubei, Sichuan and other places in China. It grows in areas with an altitude of 300 meters to 1600 meters, and generally grows on the edge of hillside forests; purple magnolia flowers are gorgeous and pleasant. It is an excellent garden and street greening plant, and it is a traditional flower and traditional Chinese medicine with a history of more than 2,000 years in China; it is not easy to transplant and maintain, it is very Precious flowers and trees. [0003] Now with the development of technology, many areas are carrying out the method of cultivating purple magnolia, and want to cultivate and plant purple magnolia in this area; traditional purple magnolia generally adopts cultiva...

AI Technical Summary

Problems solved by technology

[0003] Now with the development of technology, many areas are carrying out the method of cultivating purple magnolia, and want to cultivate and plant purple magnolia in this area; traditional purple magnolia generally adopts cultivation methods such as sowing, cutting, grafting, layering and branch propagation; The time-consuming of mode is long, and breeding is difficult for surviving; Existing some purple magnolia tissue culture modes are carried out under closed aseptic condition, required cost is bigger, and there is also explant easy browning in tissue culture, survives. The rate is not high, and it cannot be used for mass reproduction of magnolia

[0004] The patent document with the application number 201310730707.3 discloses a method of tissue culture of Magnolia japonica, which is used to provide an open condition without the need to prepare a closed aseptic environment, which saves costs; Eliminate aseptic contamination of explants, improve the survival rate of its culture; soak the explants with 2% PVP and add 2% PVP in the medium, reduce the browning of the explants and improve the survival rate of the explants The purple magnolia tissue culture method; this purple magnolia tissue culture method is simple to operate, the time is short, the cost is low, the survival rate is high, the rooting rate is high, and can realize the out-of-the-box transplanting in a short period of time, and can keep the good character of original variety, Lay the foundation for improved seed breeding and industrialized production; But it can not greatly improve the rooting rate and survival rate of Magnolia tissue culture, and can not improve the quality of Magnolia tissue culture