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Extraction method and applications of oat avenanthramides

An oat alkaloid and extraction method technology, which is applied in the field of daily-use chemicals, can solve the problems of long extraction time, low extraction efficiency, solvent residue, etc., and achieve the effects of easy industrial production, simple and easy method, and easy control of conditions

Active Publication Date: 2017-05-31
SOUTH CHINA INST OF COLLABORATIVE INNOVATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvent immersion extraction method is simple to operate and can handle a large amount, but the amount of solvent used is large, the extraction time is long, the extraction efficiency is low, and the solvent remains; supercritical CO 2 The extraction method has good selectivity, high yield, and is environmentally friendly, but the equipment is expensive and the maintenance cost is high, and alkaloids often exist in the form of salt, so that the selectivity of the extraction is not very high; the microwave extraction method has simple equipment and low cost , the extraction efficiency is high, but as the processing capacity increases, the microwave attenuates rapidly when passing through the material, resulting in a decrease in extraction efficiency
Therefore, there is no method suitable for extracting oat alkaloids in large quantities

Method used

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  • Extraction method and applications of oat avenanthramides
  • Extraction method and applications of oat avenanthramides
  • Extraction method and applications of oat avenanthramides

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Grind naked oats, pass through a 40-mesh sieve, take 5kg of pulverized oats, then add 90% ethanol solution 20 times the weight of oats to obtain a mixed solution, add 25g of cellulase, adjust the pH value to 5.5, and place in a water bath at 30°C with Stir at 50 rpm for 1 hour. Put the enzymolysis solution into the centrifuge, centrifuge at 1000rpm, remove the liquid, and keep the solid. The treated oats enter the subcritical extraction device for subcritical extraction. The extraction temperature is 50°C, the pressure is 3MPa, and propane is used for extraction. agent, propane contains 2% entraining agent, entraining agent is ethanol, reclaims extractant, discharges, and obtains oat alkaloid extract, detects by HPLC method, and the extraction rate of oat extract is 9.2%, and oat alkaloid content is 4.62%.

Embodiment 2

[0032] Grind naked oats, pass through a 40-mesh sieve, take 7.5kg of crushed oats, then add 90% ethanol solution 30 times the weight of oats to obtain a mixed solution, add 52g of pectinase, adjust the pH to 6.0, and place in a water bath at 40°C at 100rpm Stir at high speed for 1 hour. Put the enzymolysis solution into the centrifuge, centrifuge at 1000rpm, remove the liquid, and keep the solid. The treated oats enter the subcritical extraction device for subcritical extraction. The extraction temperature is 55°C, the pressure is 3MPa, and propane is used for extraction. agent, butane contains 2.5% entraining agent, the entraining agent is butanol, the extractant is recovered, and the material is discharged to obtain the oat alkaloid extract, which is detected by HPLC method, and the extraction rate of the oat extract is 9.8%, and the oat alkaloid The content is 4.38%.

Embodiment 3

[0034] Naked oats are pulverized, cross 60 mesh sieves, get the oats that 9kg pulverizes, then add oats, 80% ethanol solution of 20 times of weight, obtain mixed liquor, add 72g compound enzyme (cellulase and pectinase are 1 by mass ratio :1), adjust the pH to 5.0 and stir for 1 hour at a speed of 50 rpm in a 40° C. water bath. Put the enzymolysis solution into the centrifuge, centrifuge at 1000rpm, remove the liquid, and keep the solid. The treated oats enter the subcritical extraction device for subcritical extraction. The extraction temperature is 70°C, the pressure is 3MPa, dimethyl ether As an extractant, dimethyl ether contains 2% entrainer, and the entrainer is ethanol and butanol, and the components are 80% ethanol and 20% butanol by weight; the extractant is recovered, and the material is discharged to obtain the oat alkaloid extraction Detected by HPLC method, the extraction rate of oat extract is 10.5%, and the alkaloid content of oat is 4.72%.

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Abstract

The invention belongs to the field of daily-use chemicals, and discloses an extraction method and applications of oat avenanthramides. The method comprises the following steps: crushing oats, adding ethanol, evenly mixing and then adding enzyme, adjusting the pH value to be 5.0-6.0, performing enzymatic hydrolysis reaction under the stirring condition, centrifugally separating a reaction solution obtained after reaction is over, conducting subcritical extraction on solids obtained from centrifugal separation, and then recycling a solvent to obtain the oat avenanthramides extract. The method is simple and easy, conditions are easily controlled, the utilization rate of raw materials is high, and the industrial product is easily realized. Before extracting the oat avenanthramides, enzyme is added to perform pretreatment on oats, and the subcritical technology is used for extraction, so that the extraction rate of the oat avenanthramides is increased, and the time needed by extraction is shortened.

Description

technical field [0001] The invention belongs to the field of daily chemicals, in particular to an extraction method and application of oat alkaloids. Background technique [0002] Oats are currently widely planted in 42 countries on five continents around the world. Its planting area is second only to wheat, rice and corn. The oats planted in my country are mainly divided into husk oats and naked oats. For centuries, people have used colloidal oats The powder is used to relieve itching symptoms of the skin. In 2003, the US FDA approved colloidal oatmeal as a protective agent for alleviating various skin diseases. Alkaloids, the raw material products developed for oats and used in the field of daily chemicals, mainly focus on oat protein, oat β-glucan and their derivatives, while oat alkaloids rarely have related products. [0003] The active ingredients in natural plants are often wrapped in the inner parenchyma cells or vacuoles protected by the epidermis, and it is very d...

Claims

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Application Information

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IPC IPC(8): A61K8/9794A61Q19/08A61Q19/00C07G5/00
CPCA61K8/97A61Q19/005A61Q19/08C07G5/00
Inventor 程建华郑华生张鹏谢培镇谢明容陈杰烽黄桂州何衍健
Owner SOUTH CHINA INST OF COLLABORATIVE INNOVATION
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