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Method of separating high-purity hyperin from lotus leaf

A hyperin, high-purity technology, applied in chemical instruments and methods, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problem of high heat consumption, solvent consumption and emission, and lack of extraction of flavonoid monomers method, it is difficult to industrialize production and other issues, to achieve the effect of continuous operation, lower development and utilization costs, and reduce energy consumption

Inactive Publication Date: 2017-05-31
YANGZHOU BAOLIAN BIOLOGICAL SCI & TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The extraction of flavonoids in the prior art has the following disadvantages: 1) there are many types of flavonoids, and their functions are not the same. In development and application, functional factors should be clarified to achieve a better use effect. The separation and purification technology of leaf flavonoids is based on the total flavonoid content as an index, lacks clear functional factors, and lacks extraction methods for flavonoid monomers; 2) Whether it is the extraction of total flavonoids from lotus leaves or the extraction of hyperin from other natural plants , the current technology uses hot organic solvents, and the lotus leaf has a light specific gravity and a large volume, so the amount of solvent consumed in the leaching stage is relatively large, so that the heat consumption, solvent consumption and emissions are relatively large, which is difficult to meet Modern deep processing requirements for energy saving and emission reduction; 3) The existing extraction technology of lotus leaf flavonoids is still in the laboratory stage, and it is difficult to realize industrial production

Method used

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  • Method of separating high-purity hyperin from lotus leaf
  • Method of separating high-purity hyperin from lotus leaf
  • Method of separating high-purity hyperin from lotus leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Take 500g of crushed dry lotus leaf fragments, the size of which is about 3mm, and pre-soak the fragments with 0.09mol / L sodium hydroxide aqueous solution for 18 hours at room temperature; put the soaked lotus leaves from the feeding inlet of a small ultrasonic countercurrent extractor, Weak alkali aqueous solution is added from the outlet of the residue, the ratio of material to liquid is 1:40, the active ingredients of lotus leaves are extracted in the ultrasonic dynamic countercurrent extraction tank, the rotation speed of the auger is 0.3r.p.m, and the ultrasonic power is 3.0kW / m 3 , the lotus leaf fragments stay in the extraction tank for 35min, continuous operation; the residue is squeezed, then washed twice with clear water with a volume of 1:1 with the residue, the washing water is added to the extract, and the total flavonoid content is measured. The extraction rate was 7.89%. However, the extraction rate of total flavonoids was 8.42% in hot alcohol water extra...

Embodiment 2

[0082] Take 1 kg of crushed dry lotus leaf fragments, the size of which is about 2mm, and pre-soak the fragments with 0.07mol / L calcium hydroxide aqueous solution for 24 hours at room temperature; put the soaked lotus leaves from the feeding inlet of a small ultrasonic countercurrent extractor, Weak alkali aqueous solution is added from the outlet of the residue, the ratio of material to liquid is 1:30, the active ingredients of lotus leaves are extracted in the ultrasonic dynamic countercurrent extraction tank, the rotation speed of the auger is 0.5r.p.m, and the ultrasonic power is 2.5kW / m 3 , the lotus leaf fragments stay in the extraction tank for 30 minutes, continuous operation; the residue is squeezed, then washed twice with water with a volume of 1:1 with the residue, and the washing water is added to the extraction solution;

[0083] Adjust the pH of the extract to neutral, after standing still, use a 0.2μm microfiltration membrane to remove residual particles, the ope...

Embodiment 3

[0089] Take 5 kg of crushed dry lotus leaf fragments, the size of which is about 4 mm, and pre-soak the fragments with 0.15 mol / L potassium hydroxide aqueous solution for 12 hours at room temperature; put the soaked lotus leaves from the feeding inlet of a small ultrasonic countercurrent extractor, Weak alkali aqueous solution is added from the outlet of the residue, the ratio of material to liquid is 1:50, the active ingredients of lotus leaves are extracted in the ultrasonic dynamic countercurrent extraction tank, the rotation speed of the auger is 0.2r.p.m, and the ultrasonic power is 2.2kW / m 3 , the lotus leaf fragments stay in the extraction tank for 40 minutes, continuous operation; the residue is squeezed, then washed once with water with a volume of 1:1 with the residue, and the washing water is added to the extraction solution;

[0090] Adjust the pH of the extract to neutral, after standing still, use a 0.2μm microfiltration membrane to remove residual particles, the ...

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Abstract

The invention discloses a method of separating high-purity hyperin from a lotus leaf, and relates to a method of separating high-purity hyperin from a natural plant particularly the lotus leaf and synchronously preparing lotus leaf general flavones, in particular to a method that normal-temperature weak-base water solution ultrasonic-assisted continuous dynamic countercurrent extracting is adopted to replace conventional alcohol-water hot solvent extracting and used for extracting in combination with ultrafiltration impurity removal, nanofiltration solvent recovery and the multiple-unit macroreticular resin separation technology, and high-purity hyperin and other lotus leaf general flavones are collected through sequential elution. The method of separating high-purity hyperin from the lotus leaf is definite in functional factor, suitable for industrial production, low in cost, capable of separating high-purity hyperin from the lotus leaf in an environment-friendly and energy-saving mode and suitable for industrial development and utilization of lotus leaf flavones resources, and the extracted hyperin is high in purity.

Description

technical field [0001] The invention relates to a method for isolating high-purity hyperin from natural plants, especially lotus leaves, and synchronously producing total flavonoids of lotus leaves, in particular adopting ultrasonic-assisted continuous dynamic countercurrent leaching of normal temperature weak alkali aqueous solution to replace conventional alcohol water Hot solvent extraction, combined with ultrafiltration to remove impurities, nanofiltration solvent recovery, multi-group macroporous adsorption resin separation technology extraction, and step-by-step elution to collect high-purity hyperin and other lotus leaf total flavonoids. Background technique [0002] Extracting functional active flavonoids with medical and health value from natural plants is safe and reliable. It is very popular in the international market, and the developed health care products are also diverse. Most of the lotus leaf weight loss and blood fat-lowering medicines and health care produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61K36/62A61K127/00
CPCA61K36/62A61K2236/15A61K2236/333A61K2236/51A61K2236/53C07H1/08C07H17/07Y02P20/10
Inventor 黄阿根周爱琴高露杨振泉饶胜其黄铖骁朱杰朱映华黄娟章敏洁
Owner YANGZHOU BAOLIAN BIOLOGICAL SCI & TECH CO LTD
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