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Biomass extraction method of sericin, extract obtained by using biomass extraction method, and application of extract

A sericin and extraction method technology, which is applied in the fields of peptide preparation methods, chemical instruments and methods, medical preparations containing active ingredients, etc., can solve the problem of high cost, can not be used in one step, and increase the cost of sericin extraction in operation steps. and other problems, to achieve the effect of simple process and easy operation

Active Publication Date: 2017-05-31
EASTERN LIAONING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, patent CN201510620681.6 uses dialysis, spray drying and other methods to extract sericin protein, which has the advantage that the purity of the obtained sericin protein reaches 90-95%, but it needs to use trypsin for fermentation, and the cost is high
Currently, sericin extracted by chemical and physical methods needs to be further separated before it can be used, and cannot be used in one step
The increased operation steps put pressure on the extraction cost of sericin

Method used

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  • Biomass extraction method of sericin, extract obtained by using biomass extraction method, and application of extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) chitosan is joined in 30g / L acetic acid aqueous solution, is made into the chitosan acetic acid solution that contains chitosan concentration and is 20g / L;

[0021] (2) Get above-mentioned chitosan acetic acid solution 20mL and join in 100mL silk degumming waste water, be that the pH value of the acetic acid adjustment solution of 0.1mol / L is 5-6 with concentration, after stirring 20min with the rotating speed of 800rpm, leave standstill 20min;

[0022] (3) Filtration at room temperature, washing with water, and spray drying at 90°C.

Embodiment 2

[0024] (1) Chitosan is joined in 20g / L acetic acid aqueous solution, is made into the chitosan acetic acid solution that contains chitosan concentration and is 40g / L;

[0025] (2) Get above-mentioned chitosan acetic acid solution 20mL and join in 80mL silk degumming waste water, be that the pH value of the acetic acid adjustment solution of 0.1mol / L is 5-6 with concentration, after stirring 30min with the rotating speed of 600rpm, leave standstill 40min;

[0026] (3) Filter at room temperature, wash with water, and spray dry at 70°C.

Embodiment 3

[0028] (1) Chitosan is joined in 20g / L acetic acid aqueous solution, is made into the chitosan acetic acid solution that contains chitosan concentration and is 40g / L;

[0029] (2) Get above-mentioned chitosan acetic acid solution 20mL and join in 80mL silk degumming waste water, be that the pH value of the acetic acid adjustment solution of 0.1mol / L is 5-6 with concentration, after stirring 40min with the rotating speed of 900rpm, leave standstill for 30min;

[0030] (3) Filter at room temperature, wash with water, and spray dry at 80°C.

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Abstract

The invention discloses a biomass extraction method of sericin, an extract obtained by using the biomass extraction method, and application of the extract, and relates to the field of organic matter extraction. The biomass extraction method comprises the steps of carrying out flocculation on silk degumming wastewater by using a chitosan acetic acid solution under the condition that the pH value of the chitosan acetic acid solution is 5-6, wherein a single component of chitosan is used as a flocculating agent; recovering the sericin in the silk degumming wastewater. The biomass extraction method has the advantages of simple technology and easy operation. Generated chitosan-sericin solid can be directly applied, and the sericin and the chitosan do not need to be separated from each other, so that solid waste is not emitted, and secondary pollution is avoided.

Description

technical field [0001] The invention relates to the technical field of organic matter extraction, in particular to a method for extracting sericin from biomass and its extract and application, specifically a method for extracting sericin by using chitosan as a flocculant, and the extracted shell Glycan-sericin flocs and their applications. Background technique [0002] Sericin is a high-molecular globular protein with good skin care, humidity control, moisturizing, anti-oxidation, antibacterial, biocompatibility, degradable properties, and promotion of cell adhesion and proliferation. As a big silk producing country in the world, China has tens of thousands of tons of sericin protein discharged directly into the environment along with production wastewater every year. Because the organic content of sericin wastewater seriously exceeds the standard, resulting in high concentrations of biochemical oxygen demand (BOD) in wastewater, Chemical oxygen demand (COD) and high nitrog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/32A61K8/64A61Q17/00A61Q19/00D06M15/15D06M15/03
CPCA61K8/64A61K2800/10A61Q17/005A61Q19/00C07K14/43586D06M15/03D06M15/15D06M16/00
Inventor 路艳华程德红林杰王勃翔李佳尹丽馨
Owner EASTERN LIAONING UNIV
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