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Embryo cell active protein preparation method and its uses in skin care product

A technology of embryonic cells and active proteins, applied in skin care preparations, biochemical equipment and methods, cosmetic preparations, etc., can solve problems such as inability to obtain high-purity extracts, difficult industrial production, and harmful chemicals, and achieve Promote skin physiological functions, enhance self-repair, and nourish the skin

Inactive Publication Date: 2007-07-11
王书刚
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Problems solved by technology

This kind of deoxyribonucleic acid is not directly extracted from animal embryos through the cultivation of embryonic cells. It is difficult to form industrial production, and it is often impossible to obtain high-purity extracts, and it is easy to mix harmful chemicals to the skin during the extraction process.
[0005] At present, there are no similar products on the market, only some simple extracts of some cells or genetic engineering are added to the oil-water mixed base liquid and sold on the market as skin care and maintenance products. The main sales places are beauty centers or provide Medical institutions for skin care and restoration also need the guidance of a physician or beautician in order to achieve a certain cosmetic effect

Method used

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  • Embryo cell active protein preparation method and its uses in skin care product

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Embodiment

[0042] The active protein preparation method of the embryonic cells provided by the present embodiment is:

[0043] Cultivate embryonic cells first: use 1640 medium with 5% fetal calf or calf serum, culture conditions: 37°C carbon dioxide incubator, 5% carbon dioxide concentration; according to the growth of cells, generally the cells have covered the bottom of the bottle , when the growth shape of the cells gradually turns round, the cells are subcultured, and the subculture time is generally about three to four days.

[0044] When the cells reach a concentration of 6×10 to the 6th power (if the above concentration cannot be reached, the cell liquid can be centrifuged at 2000r / m for 30min, and the supernatant is discarded and counted) for inoculation and expansion culture, using microcarrier perfusion Bioreactors for large-scale continuous cultivation.

[0045] For the continuous culture of cell collection, at the same time, inject the same amount of culture medium as the co...

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Abstract

The invention discloses a skin nursing material of active protein extracted from embryo cell, which is characterized by the following: fusing the active protein in the embryo cell and skin cell; accelerating the regenerative ability of skin tissue cell through connecting each receptor of cell; displaying hyper high polarity due to multiple nourishing components and microelement; displaying good affinity with skin and stronger skin tissue permeability; participating or adjusting the of metabolic and physiological period of cell; activating the biological ability of cell; reinforcing the secretory ability of hyaluronic acid and glucoprotein.

Description

technical field [0001] The invention relates to a series of active protein nourishing skin care products developed on the basis of bioengineering technology combined with supercritical technology, in particular to a method for preparing active protein from embryonic cells and its application in skin care products. Background technique [0002] Most of the existing production processes of beauty cosmetics are cream products, and the main ingredients are: 1. Grease and wax; 2. Moisturizing ingredients, generally polyhydric alcohols; 3. Emulsifiers; 4. Cream thickeners; 5. Additives, such as the basic composition of general moisturizers and lotions are: DC-200, white oil, vaseline, beeswax, stearic acid, cetostearyl alcohol, lanolin, monoglyceride, IPP, BHA, borax 、K 12 , Triethanolamine, CPK, Propylene Glycol, Glycerin, Sorbitol, Preservatives, Water. The additives are mainly some plant extracts or animal extract components; this product mainly has a skin care effect, but ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12N5/06A61K8/97A61K8/64A61Q19/00B01D15/08C07K1/16
Inventor 王书刚
Owner 王书刚
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