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Culture medium capable of promoting Arnebia euchroma cells to synthesize shikonin and preparation method of culture medium

A Xinjiang comfrey, culture medium technology, applied in plant cells, fermentation and other directions, can solve the problems of restricting the industrialization process, low content of medicinal ingredients, and high cost, and achieve the effects of promoting division, maintaining osmotic pressure balance, and avoiding damage.

Inactive Publication Date: 2017-05-31
安徽檀鑫科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problem of high cost due to the low content of medicinal ingredients in the process of cell culture seriously restricts the process of industrialization

Method used

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  • Culture medium capable of promoting Arnebia euchroma cells to synthesize shikonin and preparation method of culture medium
  • Culture medium capable of promoting Arnebia euchroma cells to synthesize shikonin and preparation method of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, a kind of culture medium that promotes Shikonin to be synthesized by Xinjiang Shicao cells

[0032] The culture medium for promoting the synthesis of shikonin by Xinjiang Shikonensis cells includes a basal medium and additives added in the basal medium, and the ingredients of the additives include: zinc sulfate 0.08 μM, pyridoxine hydrochloride in terms of final concentration Pyridoxine 8mg / L, thymine 5mg / L, calcium pantothenate 1mg / L, glutathione 10μg / L, activated carbon 0.06g / L, indole acetic acid 7mg / L, mannitol 20mg / L, daidzein 50μg / L , triammonium citrate 10mg / L and xylitol 20g / L.

[0033] The basal medium is B5 medium.

[0034] Preparation:

[0035] S1 Add pyridoxine hydrochloride, thymine, calcium pantothenate, indole acetic acid, triammonium citrate and xylitol to the basal medium, stir for 30 minutes, add zinc sulfate, glutathione, activated carbon, mannitol and daidzin , continue stirring for 20 minutes to obtain a mixture;

[0036] S2 Adjust...

Embodiment 2

[0037] Embodiment 2, a kind of culture medium that promotes Shikonin to be synthesized by Xinjiang Shicao cells

[0038] The culture medium for promoting the synthesis of shikonin by Xinjiang Shikonensis cells includes a basal medium and additives added in the basal medium, and the ingredients of the additives include: zinc sulfate 0.15 μM, pyridoxine hydrochloride in terms of final concentration Pyridoxine 13mg / L, thymine 8mg / L, calcium pantothenate 5mg / L, glutathione 16μg / L, activated carbon 0.14g / L, indole acetic acid 12mg / L, mannitol 40mg / L, daidzein 75μg / L , triammonium citrate 50mg / L and xylitol 40g / L.

[0039] The basal medium is White medium.

[0040] Preparation:

[0041] S1 Add pyridoxine hydrochloride, thymine, calcium pantothenate, indole acetic acid, triammonium citrate and xylitol to the basal medium, stir for 30 minutes, add zinc sulfate, glutathione, activated carbon, mannitol and daidzin , continue stirring for 20 minutes to obtain a mixture;

[0042] S2 A...

Embodiment 3

[0043] Embodiment 3, a kind of culture medium that promotes Xinjiang shikonin cells to synthesize shikonin

[0044] The culture medium for promoting the synthesis of shikonin by Xinjiang Shikonensis cells includes a basal medium and additives added in the basal medium, and the ingredients of the additives include: zinc sulfate 0.12 μM, pyridoxine hydrochloride in terms of final concentration Pyridoxine 11mg / L, thymine 7mg / L, calcium pantothenate 3mg / L, glutathione 12μg / L, activated carbon 0.08g / L, indole acetic acid 8mg / L, mannitol 27mg / L, daidzin 62μg / L , triammonium citrate 40mg / L and xylitol 25g / L.

[0045] The basal medium is B5 medium.

[0046] Preparation:

[0047] S1 Add pyridoxine hydrochloride, thymine, calcium pantothenate, indole acetic acid, triammonium citrate and xylitol to the basal medium, stir for 30 minutes, add zinc sulfate, glutathione, activated carbon, mannitol and daidzin , continue stirring for 20 minutes to obtain a mixture;

[0048] S2 Adjust the ...

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Abstract

The invention belongs to the technical field of plant cell culture media and particularly relates to a culture medium capable of promoting Arnebia euchroma cells to synthesize shikonin and a preparation method of the culture medium. The culture medium comprises a basic culture medium and additives added into the basic culture medium, wherein the additives comprise, by final concentration, 0.08-0.15 micromole of zinc sulfate, 8-13mg / L of pyridoxine hydrochloride, 5-8mg / L of thymine, 1-5mg / L of calcium pantothenate, 10-16 microgram / L of glutathione, 0.06-0.14g / L of activated carbon, 7-12mg / L of indoleacetic acid, 20-40mg / L of mannitol, 50-75 microgram / L of daidzin, 10-50mg / L of triammonium citrate and 20-40g / L of xylitol. The culture medium is clear in component, low in price, capable of greatly shortening the production cycle of Arnebia euchroma cells and high in shikonin yield.

Description

technical field [0001] The invention belongs to the technical field of plant cell culture medium, and in particular relates to a culture medium for promoting the synthesis of shikonin by Xinjiang shikonensis cells and a preparation method thereof. Background technique [0002] The use of comfrey as medicine was first seen in "Shen Nong's Materia Medica", and "Compendium of Materia Medica" quoted Tao Hongjing as saying "it is out of Xiangyang today", which shows that comfrey has been used for medicinal purposes in ancient China. After 1990, the "Pharmacopoeia of the People's Republic of China" included 3 species of plants in the family Boraginaceae, and Xinjiang Burgess is one of them with the best quality. Xinjiang comfrey is a perennial herbaceous plant of Boraginaceae, with flat and flat hairs, thick roots, conical, twisted, and purple when dry. The flowering period is from June to August, and the fruiting period is from August to September. It likes a cool and humid cli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12P7/66
CPCC12N5/04C12P7/66
Inventor 夏国顺
Owner 安徽檀鑫科技有限公司
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