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Culture medium for culturing dental pulp stem cells and preparation method thereof

A technique for dental pulp stem cells and culture medium, which is applied to the culture medium of dental pulp stem cells and the field of preparation thereof, can solve the problems of high price and the like, and achieve the effects of high safety and increasing expansion speed.

Inactive Publication Date: 2017-05-31
JIANGXI YIXINTANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although, a serum-free medium for culturing dental pulp stem cells has been developed, but it is expensive

Method used

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  • Culture medium for culturing dental pulp stem cells and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, a kind of medium for cultivating dental pulp stem cells

[0027] The medium for cultivating dental pulp stem cells includes basal medium and additives added in the basal medium, and the ingredients of the additives include: epidermal growth factor 3 μg / L, carboxymethyl chitosan in terms of final concentration Sugar 2g / L, catalase 5mg / L, lipoic acid 0.14mg / L, coenzyme Q10 0.3μM, laminin 15μg / L, linolenic acid 2μM, nicotinamide 8μM, taurine 2mg / L, platelet-derived growth Factor 2μg / L, choline chloride 70μM, diosgenin 0.5mg / L.

[0028] The basal medium is F12 medium.

[0029] Preparation:

[0030] S1 Add epidermal growth factor, lipoic acid, laminin, linolenic acid, nicotinamide, taurine, platelet-derived growth factor and choline chloride to the basal medium, stir for 20 minutes, add carboxymethyl chitosan and Diosgenin, continue to stir for 30 minutes, let stand for 1 hour, add catalase and coenzyme Q10, stir for 30 minutes to obtain a mixture;

[0031]...

Embodiment 2

[0032] Embodiment 2, a kind of medium for cultivating dental pulp stem cells

[0033] The medium for cultivating dental pulp stem cells includes basal medium and additives added in the basal medium, and the ingredients of the additives include: epidermal growth factor 5 μg / L, carboxymethyl chitosan in terms of final concentration Sugar 4g / L, catalase 8mg / L, lipoic acid 0.18mg / L, coenzyme Q10 0.6μM, laminin 25μg / L, linolenic acid 5μM, nicotinamide 14μM, taurine 4mg / L, platelet-derived growth Factor 6μg / L, choline chloride 80μM, diosgenin 2mg / L.

[0034] The basal medium is DMEM medium.

[0035] Preparation:

[0036] S1 Add epidermal growth factor, lipoic acid, laminin, linolenic acid, nicotinamide, taurine, platelet-derived growth factor and choline chloride to the basal medium, stir for 40 minutes, add carboxymethyl chitosan and Diosgenin, continue to stir for 40 minutes, let stand for 3 hours, add catalase and coenzyme Q10, stir for 30 minutes to obtain a mixture;

[0037...

Embodiment 3

[0038] Embodiment 3, a kind of medium for cultivating dental pulp stem cells

[0039] The medium for cultivating dental pulp stem cells includes basal medium and additives added in the basal medium, and the ingredients of the additives include: epidermal growth factor 4 μg / L, carboxymethyl chitosan Sugar 3g / L, catalase 6mg / L, lipoic acid 0.16mg / L, coenzyme Q10 0.4μM, laminin 18μg / L, linolenic acid 4μM, nicotinamide 12μM, taurine 3mg / L, platelet-derived growth Factor 5μg / L, choline chloride 76μM, diosgenin 1.4mg / L.

[0040] The basal medium is F12 medium.

[0041] Preparation:

[0042] S1 Add epidermal growth factor, lipoic acid, laminin, linolenic acid, nicotinamide, taurine, platelet-derived growth factor and choline chloride to the basal medium, stir for 30 minutes, add carboxymethyl chitosan and Diosgenin, continue to stir for 35 minutes, let stand for 2 hours, add catalase and coenzyme Q10, stir for 30 minutes to obtain a mixture;

[0043] S2 Adjust the pH of the mixtu...

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Abstract

The invention belongs to the technical field of cell culture, and specifically relates to a culture medium for culturing dental pulp stem cells and a preparation method thereof. The culture medium for culturing the dental pulp stem cells comprises the a basal culture medium and an additive added into the basal culture medium, wherein the additive is prepared from the following components in final concentrations: 3 to 5 micrograms / L of epidermal growth factors, 2 to 4 g / L of carboxymethyl chitosan, 5 to 8 mg / L of catalase, 0.14 to 0.18 mg / L of lipoic acid, 0.3 to 0.6 micro mol / L of coenzyme Q10, 15 to 25 micrograms / L of laminin, 2 to 5 micro mol / L of linolenic acid, 8 to 14 micro mol / L of nicotinamide, 2 to 4 mg / L of taurine, 2 to 6 micrograms / L of platelet-derived growth factors, 70 to 80 micro mol / L of choline chloride and 0.5 to 2 mg / L of diosgenin. The culture medium for culturing the dental pulp stem cells disclosed by the invention is relatively low in cost and high in safety, and the amplification speed of the dental pulp stem cells can be obviously increased.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a medium for culturing dental pulp stem cells and a preparation method thereof. Background technique [0002] Dental defect and tooth loss due to various reasons have become the main diseases that endanger the oral cavity and even human health, and the incidence rate is increasing year by year. At present, various dental materials are mainly used to deal with tooth defect diseases. Although it can prevent the continued development of lesions to a certain extent, it cannot solve the fundamental problem. Although there are many solutions to tooth loss, the most effective and fundamental way is tooth regeneration. [0003] With the rapid development of tissue regenerative medicine, it brings hope for the effective treatment of dental defects and missing teeth. At present, many studies have used the interaction of odontogenic epithelium and odontogenic mesenchyme t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074C12N5/0775
CPCC12N5/0664C12N2500/30C12N2500/33C12N2500/34C12N2500/36C12N2500/38C12N2501/11C12N2501/135C12N2501/71C12N2501/998
Inventor 胡波钟勇财谢玉国
Owner JIANGXI YIXINTANG MEDICAL TECH
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