Biological sensor with micro-electrode and application of biological sensor to detecting salicylic acid of plants in online manner

A technology of biosensors and microelectrodes, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of sample material damage, precious material damage, and detection results without time continuity, so as to achieve reliable detection methods and accurate measurement results Effect
CN106770570AInactive Publication Date: 2017-05-31BEIJING RES CENT FOR INFORMATION TECH & AGRI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING RES CENT FOR INFORMATION TECH & AGRI
Publication Date
2017-05-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a biological sensor with a micro-electrode. The length of the micro-electrode is 4-8 cm, the length of a tip of the micro-electrode is 5-30 mm, the width of the tip of the micro-electrode is 2mm, and the thickness of the tip of the micro-electrode is 0.5mm; substrate electrodes of the micro-electrodes are made of silicon wafers, the micro-electrode is provided with a three-electrode system and comprises an Ag / AgCl reference electrode, a platinum counter electrode and a gold working electrode modified by carbon nano-tube materials, and the lengths*the widths of exposed conductive portions of the three electrodes are 0.5mm*0.5mm. The biological sensor has the advantages that the content of salicylic acid of plant bodies can be monitored by the aid of living bodies in an online manner, change conditions of the content of the salicylic acid can be truly recorded and can be analyzed in real time, detection methods are reliable, accurate measurement results can be obtained, the novel methods can be provided for detecting the salicylic acid of plants, and salicylic acid detection means can be enriched in research on the stress resistance and the disease resistance of the plants.
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Description

technical field

[0001] The invention relates to microelectrode biosensing technology, in particular to a microelectrode biosensor and its application in on-line detection of plant salicylic acid. Background technique

[0002] Salicylic acid (SA) is a natural active small molecule phenolic substance that widely exists in plant tissues. It has important pharmacological effects and participates in plant growth and development as well as physiological processes such as stress resistance and disease resistance. It has a regulatory effect on plant physiological metabolism, such as regulating the senescence of fruits.

[0003] At present, the methods for detecting salicylic acid include: ultraviolet spectrophotometry, ion chromatography, gas chromatography-mass spectrometry, and high-performance liquid chromatography. The above methods select multiple time points to sample materials, and after complex processing, finally analyze salicylic acid through instruments. The content of s...

Claims

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