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Biological sensor with micro-electrode and application of biological sensor to detecting salicylic acid of plants in online manner

A technology of biosensors and microelectrodes, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of sample material damage, precious material damage, and detection results without time continuity, so as to achieve reliable detection methods and accurate measurement results Effect

Inactive Publication Date: 2017-05-31
BEIJING RES CENT FOR INFORMATION TECH & AGRI
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AI Technical Summary

Problems solved by technology

[0003] At present, the methods for detecting salicylic acid include: ultraviolet spectrophotometry, ion chromatography, gas chromatography-mass spectrometry, and high-performance liquid chromatography. The above methods select multiple time points to sample materials, and after complex processing, finally analyze salicylic acid through instruments. The content of salicylic acid in plants at a certain time point, using the method of in vitro sampling, constitutes damage to the sample material, especially precious materials may cause complete damage; in addition, the existing technology for detecting the content of salicylic acid in plants cannot achieve continuous real-time in vivo Detection can only be done by setting the time interval in advance to take samples, so the test results do not have time continuity and cannot reflect the dynamic content changes of salicylic acid in plants

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  • Biological sensor with micro-electrode and application of biological sensor to detecting salicylic acid of plants in online manner

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 microelectrode biosensor and preparation method thereof

[0022] The microelectrode biosensor prepared in this example is 5-30mm, with a width of 2mm and a thickness of 0.5mm; the base electrode material of the microelectrode is a silicon wafer, and the microelectrode has a three-electrode system, including an Ag / AgCl reference electrode , a platinum counter electrode and a gold working electrode modified by carbon nanotube materials, the exposed conductive parts of the three electrodes are 0.5mm×0.5mm in length×width ( figure 1 ).

[0023] A gold working electrode 2, an Ag / AgCl reference electrode 3, and a platinum counter electrode 4 are sequentially arranged on the tip of the silicon wafer base 1; the other end of the silicon wafer base is wider, and three wires 5 are arranged on it, respectively The above three electrodes are connected, and the other end of the wire is used to connect to the electrochemical workstation.

[0024] The prepared microelec...

Embodiment 2

[0028] Example 2 Real-time online detection of plant salicylic acid using microelectrode biosensor

[0029] 1. Subjects

[0030] The experimental materials are potted cherry tomato seedlings that have matured, and the mature fruit on the tomato seedlings is used as the detection object. The detection time is 10:00 in the morning, and probes are inserted in different parts of the tomato fruit at random to detect salicylic acid. content.

[0031] 2. Experimental instruments and consumables

[0032] The experimental instrument used electrochemical workstation CHI760E, purchased from Shanghai Chenhua Instrument Co., Ltd., model CHI76E.

[0033] 3. Experimental method

[0034] (1) Drawing of the standard curve: connect the microelectrode biosensor prepared in Example 1 to the electrochemical workstation CHI760E, select different concentrations of salicylic acid standard solutions for microelectrode detection, the working potential is 0.9V, and start timing after the current is s...

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Abstract

The invention provides a biological sensor with a micro-electrode. The length of the micro-electrode is 4-8 cm, the length of a tip of the micro-electrode is 5-30 mm, the width of the tip of the micro-electrode is 2mm, and the thickness of the tip of the micro-electrode is 0.5mm; substrate electrodes of the micro-electrodes are made of silicon wafers, the micro-electrode is provided with a three-electrode system and comprises an Ag / AgCl reference electrode, a platinum counter electrode and a gold working electrode modified by carbon nano-tube materials, and the lengths*the widths of exposed conductive portions of the three electrodes are 0.5mm*0.5mm. The biological sensor has the advantages that the content of salicylic acid of plant bodies can be monitored by the aid of living bodies in an online manner, change conditions of the content of the salicylic acid can be truly recorded and can be analyzed in real time, detection methods are reliable, accurate measurement results can be obtained, the novel methods can be provided for detecting the salicylic acid of plants, and salicylic acid detection means can be enriched in research on the stress resistance and the disease resistance of the plants.

Description

technical field [0001] The invention relates to microelectrode biosensing technology, in particular to a microelectrode biosensor and its application in on-line detection of plant salicylic acid. Background technique [0002] Salicylic acid (SA) is a natural active small molecule phenolic substance that widely exists in plant tissues. It has important pharmacological effects and participates in plant growth and development as well as physiological processes such as stress resistance and disease resistance. It has a regulatory effect on plant physiological metabolism, such as regulating the senescence of fruits. [0003] At present, the methods for detecting salicylic acid include: ultraviolet spectrophotometry, ion chromatography, gas chromatography-mass spectrometry, and high-performance liquid chromatography. The above methods select multiple time points to sample materials, and after complex processing, finally analyze salicylic acid through instruments. The content of s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/26
CPCG01N27/327G01N27/26
Inventor 侯佩臣王成李爱学王晓冬胡叶宋鹏罗斌何璐璐高权潘大宇
Owner BEIJING RES CENT FOR INFORMATION TECH & AGRI
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