Biological sensor with micro-electrode and application of biological sensor to detecting salicylic acid of plants in online manner
A technology of biosensors and microelectrodes, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of sample material damage, precious material damage, and detection results without time continuity, so as to achieve reliable detection methods and accurate measurement results Effect
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Embodiment 1
[0021] Embodiment 1 microelectrode biosensor and preparation method thereof
[0022] The microelectrode biosensor prepared in this example is 5-30mm, with a width of 2mm and a thickness of 0.5mm; the base electrode material of the microelectrode is a silicon wafer, and the microelectrode has a three-electrode system, including an Ag / AgCl reference electrode , a platinum counter electrode and a gold working electrode modified by carbon nanotube materials, the exposed conductive parts of the three electrodes are 0.5mm×0.5mm in length×width ( figure 1 ).
[0023] A gold working electrode 2, an Ag / AgCl reference electrode 3, and a platinum counter electrode 4 are sequentially arranged on the tip of the silicon wafer base 1; the other end of the silicon wafer base is wider, and three wires 5 are arranged on it, respectively The above three electrodes are connected, and the other end of the wire is used to connect to the electrochemical workstation.
[0024] The prepared microelec...
Embodiment 2
[0028] Example 2 Real-time online detection of plant salicylic acid using microelectrode biosensor
[0029] 1. Subjects
[0030] The experimental materials are potted cherry tomato seedlings that have matured, and the mature fruit on the tomato seedlings is used as the detection object. The detection time is 10:00 in the morning, and probes are inserted in different parts of the tomato fruit at random to detect salicylic acid. content.
[0031] 2. Experimental instruments and consumables
[0032] The experimental instrument used electrochemical workstation CHI760E, purchased from Shanghai Chenhua Instrument Co., Ltd., model CHI76E.
[0033] 3. Experimental method
[0034] (1) Drawing of the standard curve: connect the microelectrode biosensor prepared in Example 1 to the electrochemical workstation CHI760E, select different concentrations of salicylic acid standard solutions for microelectrode detection, the working potential is 0.9V, and start timing after the current is s...
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