Method and kit for simultaneously detecting multi vitamins in dry blood spot card

Abstract

The invention discloses a method for simultaneously detecting multi vitamins in a dry blood spot card. The method comprises the following steps: (1) mixing a dry blood spot card specimen and an extracting solution; oscillating and incubating; then centrifuging and taking supernatant; (2) after uniformly mixing the supernatant obtained by the step (1) with a mobile phase and an internal standard mixed solution, detecting by adopting a tandem mass spectrometry; comparing ion intensity of the vitamins and internal standard of the vitamins to obtain the content of the vitamins in blood serum to be detected or blood plasma to be detected. Furthermore, the invention also discloses a kit for simultaneously detecting the multi vitamins in the dry blood spot card; the kit comprises the internal standard mixed solution, the extracting solution, the mobile phase and a probe washing solution. According to the method disclosed by the invention, multi water-soluble and fat-soluble vitamins are extracted from the dry blood spot card and more than ten vitamins in one specimen can be simultaneously quantified within 2min to 3min by utilizing a tandem mass spectrometer; a detection period is remarkably shortened, and a detection result of the vitamins has higher precision and accuracy.

Description

technical field [0001] The invention relates to vitamin detection technology, in particular to a method and kit for simultaneously detecting multiple vitamins in dried blood filter paper. Background technique [0002] Vitamins are a class of organic substances that humans and animals must obtain from food in order to maintain normal physiological functions, and play an important role in the growth and metabolism of the human body. The lack of vitamins in the body will cause metabolic disorders and various diseases, which are collectively referred to as vitamin deficiency. At present, vitamins can be divided into fat-soluble vitamins and water-soluble vitamins according to their solubility. Water-soluble vitamins include B vitamins, vitamin C, etc.; fat-soluble vitamins include vitamins A, D, E, and K. [0003] Among the fat-soluble vitamins, vitamin A is the main component of the photosensitive substance of the photoreceptor cells in the retina. Vitamin A deficiency can cau...

AI Technical Summary

Problems solved by technology

Regarding the detection of vitamins, the traditional method is the microbial method, which has high sensitivity and accurate results, but this method has many limitations: the entire experiment cycle is long, the repeatability of batch test results is poor, and the test results have large errors, and each time only The detection of a single vitamin type; and recently, the development of liquid phase tandem mass spectrometry has brought the detection of vitamins into a new field. popularized in the diagnosis of disease

[0006] At present, there is only a method for the determination of a single vitamin in human serum in China, and serum is liquid during transportation, and it needs to be filled in a container and the serum needs to be stabilized in a certain temperature range. In this way, some containers are made of glass, which is fragile and at the same time , the container takes up a lot of space; to maintain the serum at a certain temperature range, it is necessary to purchase a refrigerator and other constant temperature devices, and the refrigerator will also take up a large space. Therefore, the cost of transporting serum is too high, and the quantity transported each time is also very limited.

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