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Method capable of quickly detecting paraquat in beverages

A technology for paraquat and beverages, which is applied in the preparation of test samples, measuring devices, analytical materials, etc., can solve the problems of affecting the test results, interfering with the Raman spectrum detection substrate, complex components, etc., achieving high sensitivity, effective qualitative, The effect of low detection limit

Inactive Publication Date: 2017-06-09
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Beverages, especially fruit juices and vegetable juices, have complex components, which will interfere with the detection substrate of Raman spectroscopy and affect the detection results

Method used

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  • Method capable of quickly detecting paraquat in beverages
  • Method capable of quickly detecting paraquat in beverages
  • Method capable of quickly detecting paraquat in beverages

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of gold nanoparticle sol

[0032] 100mL concentration of 0.01wt% chloroauric acid is poured into the flask, placed on a constant temperature magnetic stirrer, stirred vigorously at 1100rpm and heated to solution boiling, then adding 0.7mL concentration of 1wt% trisodium citrate solution, simultaneously with Vigorously stirred at 1100rpm and kept boiling for 15min, and the reaction was quickly terminated in an ice bath. After the solution was cooled, 15 mL of the prepared gold nanoparticle hydrosol was taken, centrifuged and the supernatant (about 14 mL) was discarded, and the gold nanoparticle sol in the lower layer was taken to be used as the substrate for surface-enhanced Raman spectroscopy for subsequent detection.

[0033] The prepared gold nanoparticle sol was detected, and the TEM results were as follows: figure 2 , the average particle size of the prepared gold nanoparticles is 64±6nm, the size is uniform, and the dispersion is good.

Embodiment 2

[0034] Example 2 Detection of Paraquat Standard Solution by Gold Nanoparticle Sol

[0035] Firstly prepare paraquat standard aqueous solutions with gradient concentrations (10 μg / L, 5 μg / L, 2 μg / L, 1 μg / L, 0.5 μg / L, 0.2 μg / mL, 0 μg / mL), using a PVDF needle with a pore size of 0.22 μm Filter with a filter membrane, then take 50 μL and place it in a 1.5 mL centrifuge tube, add an equal volume of gold nanoparticle sol (prepared in Example 1) to mix, vortex for 10 seconds; take 5 μL of the mixed solution and add it dropwise to a clean silicon wafer , after drying, use a micro-Raman spectrometer for surface-enhanced Raman spectroscopy. The power of the micro-Raman spectrometer is 80mW, the laser wavelength is 780nm, and the collected spectrum ranges from 550 to 2000cm -1 .

[0036] The test results of paraquat standard solution with gradient concentration are as follows: image 3 As shown, the Raman spectrum of paraquat standard is shown as figure 1 shown. according to image...

Embodiment 3

[0037] Example 3 Direct Determination of Paraquat Content in Apple Juice

[0038] Add paraquat mother liquor to apple juice to prepare apple juice containing paraquat with a gradient concentration of 0-20 μg / mL, and filter it with a PVDF needle filter membrane with a pore size of 0.22 μm; take 50 μL in a 1.5 mL centrifuge tube, and The volume of the gold nanoparticle colloid (prepared in Example 1) was mixed, vortexed for 10 s, and 5 μL of the mixed liquid was dropped on a clean silicon wafer, dried, and measured by a micro-Raman spectrometer. The power of the micro-Raman spectrometer is 80mW, the laser wavelength is 780nm, and the scanning range is 550-2000cm -1 .

[0039] The obtained SERS spectrum is as follows Image 6 As shown, it can be seen that the direct mixing method was used to detect the content of paraquat in apple juice, and the minimum detection concentration was 5 μg / mL.

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Abstract

The invention belongs to the field of pesticide detection and provides a method capable of quickly detecting paraquat in beverages. The method capable of quickly detecting the paraquat in the beverages includes the steps of diluting samples of beverages such as fruit juice and vegetable juice, using gold nanoparticle sol as a substrate, and detecting the paraquat in the samples through surface-enhanced Roman spectroscopy. The method capable of quickly detecting the paraquat in the beverages has the advantages that the residual paraquat in the samples can be detected qualitatively and quantitatively quickly and effectively; the method is high in sensitivity, low in detection limit and capable of detecting the 0.1 microgram / mL paraquat effectively only within a few minutes, and can only detect the above 5 micrograms / mL paraquat in undiluted samples, so that the paraquat can be detected quickly, practically, efficiently and economically.

Description

technical field [0001] The invention relates to a method for detecting paraquat, in particular to a method for rapidly detecting paraquat in beverages. Background technique [0002] With the advancement of science and technology, the use of pesticides in agriculture has become increasingly widespread. However, pesticide residues in agricultural products are harmful to personal safety. Pesticide residues in agricultural products gradually accumulate in the human body after being eaten, causing chronic poisoning and endangering personal safety. [0003] As a quick-acting contact-killing quaternary ammonium salt herbicide, paraquat has high-efficiency herbicide effect and is widely used in the planting process of fruit and vegetable crops. At present, more than 130 countries and regions in the world use pesticides containing paraquat in large quantities. Because paraquat has a stable chemical molecular structure and is not easy to degrade, it remains in plant tissues for a l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/38G01N1/34
CPCG01N21/658G01N1/34G01N1/38G01N2201/06113
Inventor 樊玉霞黄轶群赖克强罗海瑞
Owner SHANGHAI OCEAN UNIV
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