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Pericarpium citri reticulatae viride reference extract preparation method

A technology of extract and green bark, which is applied in the field of preparation of green bark control extract, to achieve uniform use, ensure consistency, and stable properties

Active Publication Date: 2017-06-09
广州科曼生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But also because the Chinese medicine reference extract has the above-mentioned specific requirements, there is no or no mature method for preparing the reference extract of Qingpi in the prior art.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] Embodiment 1: the preparation of Qingpi reference extract

[0113] The present embodiment provides the preparation method of Qingpi reference extract of the present invention, and its method flow chart is as follows Figure 21 shown.

[0114] 1: Screening of raw materials and medicinal materials

[0115] In this step, the combination of thin layer chromatography and high performance liquid chromatography is used to screen the raw medicinal materials.

[0116] 1 Thin-layer chromatography screening of raw materials

[0117] 1.1 Preparation of the test product

[0118] Preparation of chemical reference solution: take hesperidin, nobietin, and tangeretin reference substances, accurately weigh them, and prepare 0.1 mg / ml solutions with methanol respectively.

[0119] The preparation of medicinal material need testing solution: get green peel A, green peel B, green peel C, green peel D, green peel E, green peel F, green peel G, green peel H, green peel I, green peel J med...

Embodiment 2

[0242] The character analysis of embodiment 2 Qingpi contrast extract

[0243] 1. Appearance: The Qingpi control extracts QPERS-1 and QPERS-2 obtained in Example 1 are both yellow-green dry powders.

[0244] 2. Moisture determination: Carry out according to Appendix IX G of the Chinese Pharmacopoeia of the 2010 edition (reduced pressure drying method). The test result shows that the water content of QPERS1 is 2.70%, and the water content of QPERS2 is 2.30%.

[0245] 3. Consistency test: prepare 10 batches of Sihua Qingpi ERS (QPERS-1) (6 batches) and a Qingpi ERS (QPERS-2) (4 batches) according to the method of Example 1, and each batch is measured The TLC detection results for flavonoid glycosides and flavonoid aglycone components showed little difference, and the fluorescent spots of hesperidin, tangeretin and nobiletin were all clearly displayed, and the distribution was very consistent; The content of hesperidin was detected by liquid chromatography within the standard r...

Embodiment 3

[0253] This example provides the application of the reference extract of Pseudomonas chinensis in Example 1.

[0254] Commercial medicinal materials were analyzed by thin-layer chromatography and high-performance liquid chromatography, respectively, with chemical reference substance and Qingpi reference extract as reference substances.

[0255] 1 TLC

[0256] 1.1 Sample preparation

[0257] Chemical reference solution: Accurately weigh 2.5mg each of hesperidin, nobiletin, tangeretin, neohesperidin, and naringin, put them in a 25ml volumetric flask, add 20ml of methanol, and process with ultrasonic (500w) for 15 minutes to Dissolve completely, let cool, make up to 25ml mark and pass through 0.22μm filter membrane.

[0258] Qingpi reference extract solution: Accurately weigh 20 mg each of Qingpi reference extracts QPERS-1 and QPERS-2 prepared in Example 1, put them in a 2ml measuring bottle, add 1.5ml of methanol respectively, ultrasonically (500w) for 15 minutes, put Cool, a...

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Abstract

The invention provides a pericarpium citri reticulatae viride reference extract preparation method. The method includes that pericarpium citri reticulatae viride medicinal material powder different in source or batch is extracted for multiple times to obtain pericarpium citri reticulatae viride extracts different in source or batch, and the pericarpium citri reticulatae viride extracts different in source or batch are blended to obtain pericarpium citri reticulatae viride reference extracts. The pericarpium citri reticulatae viride reference extract preparation method is convenient and simple in operation, low in cost, high in repeatability and high in extraction rate. Uniformity of the pericarpium citri reticulatae viride reference extracts, different in source or batch, prepared according to the method is guaranteed; by adoption of representative medicinal materials as raw materials, obtained products are representative and capable of directly reflecting quality of to-be-detected medicinal materials when serving as the reference extracts, and character stability and uniformity and convenience in use are realized.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a method for preparing a reference extract of Qingpi. Background technique [0002] Qingpi is the pericarp of dried young fruit or immature fruit of Rutaceae plant orange and its cultivars. From May to June, the young fruit that falls from the sun is collected and dried, which is commonly called "Geqingpi"; from July to August, the immature fruit is harvested, cut into four petals on the pericarp to the base, remove all the petals, and dry in the sun , Xi called "Sihua Qingpi". [0003] Qingpi has a long history of medicinal use, and is commonly used as a medicine for soothing the liver and breaking qi, eliminating accumulation and stagnation. In the 2015 edition of the Chinese Pharmacopoeia Quality Standard issued by the National Pharmacopoeia Committee, the content of hesperidin, one of the active ingredients of green peel, is stipulat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 郭隆钢许舜军孙帅许艺镌巫少娟赵岳锐谢培山许铮弟
Owner 广州科曼生物科技有限公司
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