Method for culturing euscaphis japonica new germ plasm through space mutation breeding and proliferation culture medium
A proliferation medium and space mutagenesis technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of low seedling emergence rate, restrictions on the production and utilization of Corvus japonica seedlings, failure to maintain excellent variation characteristics, etc. Problems, to achieve ease of operation, improve the frequency of somatic embryos and regenerated plants of S. arvensis, and amplify the effects of spatial mutagenesis
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Embodiment 1
[0033] A method of space mutation breeding for cultivating new germplasm of Arvenia japonica. Select mature and plump Arvenia japonica seeds, carry the "Shenzhou 10" spacecraft into space for 15 days, carry out space mutation breeding, and after sowing seedlings, obtain annual For seedlings, select the second side branch bud eye, clean it with detergent, rinse it in running water for 10 minutes, immerse it in 75% ethanol for 30 seconds, rinse it three times with sterile water, immerse it in 0.1% mercuric chloride for 8 minutes, rinse it with sterile water for 5 minutes. Next, drain the water, cut off the 03-0.5cm part of the terminal section soaked by the drug, cut off the leaves, and inoculate in the improved MS+6-BA (1.0mg / L)+NAA (0.2mg / L)+white sugar (25g / L ) + agar (7g / L) culture medium to induce callus tissue, transfer to the same culture medium again after 30 days, wait for the plant to grow to 3-4cm high, select the robust rootless seedlings and inoculate them into the r...
Embodiment 2
[0035] A method of space mutation breeding for cultivating new germplasm of Arvenia japonica. Select mature and plump Arvenia japonica seeds, carry the "Shenzhou 10" spacecraft into space for 15 days, carry out space mutation breeding, and after sowing seedlings, obtain annual For seedlings, cut off the bud eye of the second lateral branch, rinse with running water for 15 minutes, immerse in 75% ethanol for 30 seconds, rinse with sterile water for 3 times, immerse in 0.1% mercuric chloride for 8 minutes, rinse with sterile water for 5 times, drain the water, and cut off the branches. Cut off the part of the terminal segment 03~0.5cm soaked by the medicine, cut off the leaves, inoculate on the medium of improved MS+0.5mg / L 6-BA+0.1mg / L NAA+25g / L white sugar+7g / L agar to induce callus After 20 days, transfer to the medium of improved MS+1.0mg / L 6-BA+0.2mg / L NAA+25g / L white sugar+8g / L agar to obtain regenerated seedlings. After 25-30 days, the growth and quality characteristics of...
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