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Curcumin hyaluronic acid nano-micelle for treating rheumatoid arthritis as well as preparation method and application thereof

A technology of hyaluronic acid and nanomicelles, which is applied in the field of biomedical materials, can solve the problems of poor treatment effect, adverse reactions and side effects, and achieves the effects of low cost, simple preparation method and proliferation promotion.

Inactive Publication Date: 2017-06-13
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a low-cost, high-efficiency, humanized drug for the treatment of rheumatoid arthritis in the above-mentioned prior art that has a poor therapeutic effect and easily causes adverse reactions and side effects. Arthritis curcumin hyaluronic acid nano-micelle and its preparation method and application, the drug has good biocompatibility and no in vivo toxicity, can quickly promote the healing of rheumatoid arthritis, and at the same time can play a role in the joint The role of lubrication and protection

Method used

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  • Curcumin hyaluronic acid nano-micelle for treating rheumatoid arthritis as well as preparation method and application thereof
  • Curcumin hyaluronic acid nano-micelle for treating rheumatoid arthritis as well as preparation method and application thereof
  • Curcumin hyaluronic acid nano-micelle for treating rheumatoid arthritis as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: Preparation of hyaluronic acid curcumin nano-micelle:

[0060] (1) Dissolve 100 mg of hyaluronic acid in 50 mL of formamide solution, then add 25 mL of DMSO solution, fully dissolve under magnetic stirring, and place in a constant temperature water bath at 60°C for 24 h.

[0061] (2) Purify the resulting solution under nitrogen for 30 minutes to remove oxygen from the solution.

[0062] (3) Add 203 mg of 4-dimethylaminopyridine (DMAP) and 101.5 mg of dicyclohexylcarbodiimide to the above solution to dissolve fully.

[0063] (4) Dissolve 72 mg curcumin in 10 mL DMSO to prepare curcumin solution.

[0064] (5) The prepared curcumin solution was added dropwise to the above solution under nitrogen protection, and stirred for 24 h to obtain a hyaluronic acid solution containing grafted curcumin.

[0065] (6) Use a dialysis membrane to perform water dialysis on the solution obtained in (5) above, and replace the aqueous solution every day until the unreacted co...

Embodiment 2

[0067] Embodiment 2: Preparation of hyaluronic acid curcumin nano-micelle:

[0068] (1) Dissolve 100 mg of hyaluronic acid in 50 mL of formamide solution, then add 25 mL of DMSO solution, fully dissolve under magnetic stirring, and place in a constant temperature water bath at 60°C for 24 h.

[0069] (2) Purify the resulting solution under nitrogen for 30 minutes to remove oxygen from the solution.

[0070] (3) Add 101.5 mg of 4-dimethylaminopyridine (DMAP) and 50.75 mg of dicyclohexylcarbodiimide to the above solution to fully dissolve.

[0071] (4) Dissolve 36 mg curcumin in 5 mL DMSO to prepare curcumin solution.

[0072] (5) The prepared curcumin solution was added dropwise to the above solution under nitrogen protection, and stirred for 24 h to obtain a hyaluronic acid solution containing grafted curcumin.

[0073] (6) Use a dialysis membrane to perform water dialysis on the solution obtained in (5) above, and replace the aqueous solution every day until the unreacted c...

Embodiment 3

[0075] Embodiment 3: Preparation of hyaluronic acid curcumin nano-micelle:

[0076] (1) Dissolve 100 mg of hyaluronic acid in 50 mL of formamide solution, then add 25 mL of DMSO solution, fully dissolve under magnetic stirring, and place in a constant temperature water bath at 60°C for 24 h.

[0077] (2) Purify the resulting solution under nitrogen for 30 minutes to remove oxygen from the solution.

[0078] (3) Add 50.75 mg of 4-dimethylaminopyridine (DMAP) and 25.38 mg of dicyclohexylcarbodiimide to the above solution to dissolve fully.

[0079] (4) Dissolve 18 mg curcumin in 2.5 mL DMSO to prepare curcumin solution.

[0080] (5) The prepared curcumin solution was added dropwise to the above solution under nitrogen protection, and stirred for 24 h to obtain a hyaluronic acid solution containing grafted curcumin.

[0081] (6) Use a dialysis membrane to perform water dialysis on the solution obtained in (5) above, and replace the aqueous solution every day until the unreacted...

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Abstract

The invention discloses a curcumin hyaluronic acid nano-micelle for treating rheumatoid arthritis. A compound is formed through esterification reaction of curcumin and a hyaluronic acid solution and is subjected to self-assembling in water solution by virtue of hydrophilic and hydrophobic action forces so as to form the nano-micelle. The invention fuehrer provides a preparation method and application of the curcumin hyaluronic acid nano-micelle. The curcumin hyaluronic acid nano-micelle has the beneficial effects that the curcumin hyaluronic acid nano-micelle is prepared by virtue of a nanometer technique and is used for treating the rheumatoid arthritis; the preparation method is simple and low in cost and has no requirement on special requirement; the curcumin hyaluronic acid nano-micelle has good biocompatibility and has no toxic and side effects in vivo; the curcumin hyaluronic acid nano-micelle further has the efficacies of obviously lubricating and protecting joints and resisting inflammation edema, after the curcumin hyaluronic acid nano-micelle is used for treating the rheumatoid arthritis for two weeks, swollen joints can be completely restored to an original state, and joint tissue surfaces are normal; and compared with traditional treatment drugs, the healing cycle can be greatly shortened, and the heating effect is improved.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a curcumin hyaluronic acid nano-micelle for treating rheumatoid arthritis, a preparation method and application thereof. Background technique [0002] Rheumatoid arthritis is a chronic systemic autoimmune disease mainly characterized by joint lesions. The main clinical manifestation is joint swelling and pain, which in turn leads to the destruction of articular cartilage and bone, and finally leads to joint stiffness, deformity and dysfunction. Approximately 1% of the world's population suffers from the disease, with more than 3 million new cases diagnosed each year. The etiology of rheumatoid arthritis is still unclear, and it may be related to cytokines (IL-1, TNF-α, IL-6, IL-8), cell adhesion molecules, matrix metalloproteinases, etc. Treatment for rheumatoid arthritis focuses on reducing joint pain and swelling, reducing bone deformities, and restoring normal ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K31/12A61K47/61A61P19/02A61K9/19
CPCA61K9/19A61K31/12
Inventor 范增杰李杰刘斌
Owner LANZHOU UNIVERSITY
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