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Preparation method for optical table for observing osteocyte

An optical platform, bone cell technology, applied in the fields of medical science, pharmaceutical formulations, prostheses, etc., can solve the problem that the growth and state of cells cannot be observed with an optical microscope.

Inactive Publication Date: 2017-06-13
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the early stage of the experiment, the growth and state of the cells could not be observed with an optical microscope, which caused great inconvenience in the inspection stage of the early application of the material.

Method used

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  • Preparation method for optical table for observing osteocyte
  • Preparation method for optical table for observing osteocyte
  • Preparation method for optical table for observing osteocyte

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0031] Weigh 0.5968g of calcium nitrate tetrahydrate and dissolve it completely with 50mL of deionized water to obtain a calcium nitrate solution.

[0032] Weigh 0.1996g of diammonium hydrogen phosphate and dissolve it completely with 50mL of deionized water to obtain ammonium dihydrogen phosphate solution.

[0033] Under the condition of magnetic stirring, the ammonium dihydrogen phosphate solution was slowly added to the calcium nitrate solution for reaction, and the pH of the reaction system was adjusted to 10 by ammonia water during the addition process to obtain a white hydroxyapatite suspension.

[0034] After the treated glass substrate is placed in the polytetrafluoroethylene reaction liner, hydroxyapatite suspension is added to the liner.

[0035] Put the autoclave in an oven for hydrothermal reaction at 180°C for 24 hours. After the reaction was completed and cooled to room temperature, the sample was ultrasonically washed for 5 minutes to remove the unstable hydroxy...

Embodiment example 2

[0037] Weigh 0.2984g calcium nitrate tetrahydrate and dissolve completely with 50mL deionized water to obtain calcium nitrate solution.

[0038] Weigh 0.0998g of diammonium hydrogen phosphate and dissolve it completely with 50mL of deionized water to obtain ammonium dihydrogen phosphate solution.

[0039] Under the condition of magnetic stirring, the ammonium dihydrogen phosphate solution was slowly added to the calcium nitrate solution for reaction, and the pH of the reaction system was adjusted to 10 by ammonia water during the addition process to obtain a white hydroxyapatite suspension.

[0040] After the treated glass substrate is placed in the polytetrafluoroethylene reaction liner, hydroxyapatite suspension is added to the liner.

[0041] Put the autoclave in an oven for hydrothermal reaction at 180°C for 24 hours. After the reaction was completed and cooled to room temperature, the sample was ultrasonically washed for 5 minutes to remove the unstable hydroxyapatite att...

Embodiment example 3

[0043] Weigh 0.1492g of calcium nitrate tetrahydrate and dissolve it completely with 50mL of deionized water to obtain a calcium nitrate solution.

[0044] Weigh 0.0449g of diammonium hydrogen phosphate and dissolve it completely with 50mL of deionized water to obtain ammonium dihydrogen phosphate solution.

[0045] Under the condition of magnetic stirring, the ammonium dihydrogen phosphate solution was slowly added to the calcium nitrate solution for reaction, and the pH of the reaction system was adjusted to 10 by ammonia water during the addition process to obtain a white hydroxyapatite suspension.

[0046] After the treated glass substrate is placed in the polytetrafluoroethylene reaction liner, hydroxyapatite suspension is added to the liner.

[0047] Put the autoclave in an oven for hydrothermal reaction at 180°C for 24 hours. After the reaction was completed and cooled to room temperature, the sample was ultrasonically washed for 5 minutes to remove the unstable hydroxy...

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Abstract

The invention relates to a preparation method for an optical table for observing osteocyte and belongs to the technical field of compounding of a compound biological material. According to the invention, a method for coating hydroxyapatite films on optical substances through a hydrothermal process is adopted; the coated substance materials can be used for conveniently observing the condition of growth and differentiation of the cells on the surface of hydroxyapatite; and meanwhile, the interaction of human osteocyte and orthopaedic drug can be observed.

Description

technical field [0001] The invention belongs to the technical field of composite biomaterial synthesis, in particular to the fabrication technology of an optical platform for observing the interaction between bone cells and orthopedic drugs. Background technique [0002] Hydroxyapatite (Hydroxyapatite, HA) is the most important component of human bones. The hydroxyapatite synthesized by the hydrothermal method not only has a nano-scale crystal needle structure, but also has very good biocompatibility, and has very broad application value in the field of medical support materials. The artificially synthesized hydroxyapatite powder sample has the advantages of special surface properties, bioactivity, biodegradability, osteoconductivity, and non-immunogenicity. After implanting in the human body, calcium and phosphorus are released from the material under the action of body fluids. Surface, and form a chemical bond with human bone tissue, participate in the formation of new ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/32A61L27/10A61L27/50
CPCA61L27/32A61L27/10A61L27/50A61L2400/18
Inventor 朱沛志刘冠雄赵科薛才宝
Owner YANGZHOU UNIV
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