A biological scaffold material for root regeneration, preparation method and application thereof

A biological scaffold and scaffold material technology, applied in the field of medical materials, can solve the problems of weak stem cell directional differentiation function, lack of cell adhesion and differentiation, unable to maintain stem cell activity, etc., and achieve good biocompatibility, natural ingredients, and volume. and the effect of increasing the inner surface area of ​​the tubules

Active Publication Date: 2019-11-05
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these scaffold materials still have different defects, such as: the immunogenicity of allogeneic TDM and whether it causes disease transmission remains to be verified; although TDM retains some active protein components in natural dentin, the number of these proteins is small, and it can induce stem cell orientation. The function of differentiation is weak; the root regeneration scaffold material formed by inorganic substances such as hydroxyapatite and tricalcium phosphate lacks the function of loading active ingredients, cannot maintain the activity of stem cells, and does not have the microstructure to promote cell adhesion and differentiation
These defects on the one hand lead to the low success rate of the above-mentioned scaffold materials when applied to tooth root regeneration; on the other hand, even if the scaffold materials survive, the amount of hard tissue formed is often limited, and most of them are bone-like tissues rather than real teeth. Essence and cementum
Therefore, there is currently no ideal scaffold material capable of achieving biomimetic root regeneration.

Method used

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  • A biological scaffold material for root regeneration, preparation method and application thereof
  • A biological scaffold material for root regeneration, preparation method and application thereof
  • A biological scaffold material for root regeneration, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation of dentin scaffold material derived from bovine teeth.

[0033] (1) Take bovine milk incisors, intercept the upper 1 / 3 part of the root, and remove the pulp tissue;

[0034] (2) Remove the pulp and part of the predentin along the inner side of the pulp cavity of the root, and remove the cementum and part of the dentin according to the shape of the root to obtain a 1 mm thick and 1.1 cm long dentin. Scaffolds;

[0035] (3) The scaffold material was placed in water, treated in an ultrasonic oscillator for 30 minutes, and dried at 60°C.

[0036] (4) Place the dried scaffold material in an aqueous formic acid solution with a mass fraction of 20%, and quickly put the whole system under a negative pressure of 5Kpa for 3 minutes.

[0037] (5) Take out the scaffold material treated in step (4), quickly blow off the acid on its surface, and place it at room temperature for 10 minutes.

[0038] (6) The scaffold material was placed in water, treated in an...

Embodiment 2

[0042] Example 2: Preparation of dentin scaffold material derived from porcine teeth.

[0043] (1) Take pig mandibular lateral incisors, intercept the upper 1 / 3 part of the root, and remove the pulp tissue;

[0044] (2) Remove the pulp and part of the predentin along the inside of the pulp cavity of the root, and remove the cementum and part of the dentin according to the shape of the root to obtain a dentin layer with a thickness of 1.5 mm and a length of 1.2 cm the support material;

[0045] (3) The scaffold material was placed in water, treated in an ultrasonic oscillator for 30 minutes, and dried at 80°C.

[0046] (4) Place the dried scaffold material in an aqueous formic acid solution with a mass fraction of 20%, and quickly put the whole system under a negative pressure of 5Kpa for 1 minute.

[0047] (5) Take out the scaffold material treated in step (4), quickly blow off the acid on its surface, and place it at room temperature for 15 minutes.

[0048] (6) The scaffo...

Embodiment 3

[0052] Example 3: Preparation of dentin scaffold material derived from human teeth.

[0053] (1) Take the mandibular first premolar, cut off the upper 1 / 2 part of the root, and remove the pulp tissue;

[0054] (2) Remove the pulp and part of the predentin along the inner side of the pulp cavity of the tooth root, and remove the cementum and part of the dentin according to the shape of the root to obtain a 0.5 mm thick and 5 mm long dentin. Scaffolds;

[0055] (3) The scaffold material was placed in water, treated in an ultrasonic oscillator for 20 minutes, and dried at 50°C.

[0056] (4) Place the dried scaffold material in an aqueous formic acid solution with a mass fraction of 10%, and quickly place the whole system under a negative pressure of 5Kpa for 2 minutes.

[0057] (5) Take out the scaffold material treated in step (4), quickly blow off the acid on its surface, and place it at room temperature for 30 minutes.

[0058] (6) The scaffold material was placed in water,...

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Abstract

The invention relates to a biological scaffold material for regenerating fang, a preparation method and an application thereof at the aspects of fang regeneration or bone regeneration, drug slow release and control release and belongs to the technical field of medical materials. The scaffold material is derived from natural dentin; after micro-selective acid etching and temperature control roasting treatment, the organic matter is removed, the apatite inorganic constituent in the natural dentin is maintained, a functional micron-order tubular structure is formed on the surface of the material, the adherence of cells to the material surface is enhanced and the cell process is boosted to grow into the material, so that the forming of well-aligned dentinal tubule in the fang regeneration process is boosted. The scaffold material has excellent biocompatibility and biological function, can be used as a drug and gene carrier and can realize the drug and gene slow release function in the fang regeneration process.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a biological scaffold material for tooth root regeneration, a preparation method thereof, and its application in tooth root regeneration or bone regeneration, slow release and controlled release of drugs. Background technique [0002] Tooth loss due to periodontitis, caries, trauma and other reasons is one of the most common clinical manifestations in oral cavity. Tooth loss not only seriously damages the function of oral mastication, but also affects pronunciation, facial shape, etc., which seriously affects the physical and mental health of patients. How to best restore missing teeth has always been an important issue in the field of stomatology. [0003] At present, the methods commonly used in dental clinics to restore missing teeth include removable dentures, fixed dentures and implant dentures. Among them, removable dentures often cause foreign body ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/12A61L27/50A61L27/56A61L27/54
CPCA61L27/12A61L27/3608A61L27/365A61L27/3695A61L27/50A61L27/54A61L27/56A61L2300/112A61L2300/232A61L2300/252A61L2300/412A61L2300/602A61L2430/02A61L2430/12A61L2430/40
Inventor 倪世磊孙宏晨张恺杨柏李道伟李祥伟李毅
Owner JILIN UNIV
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