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Preparation method for oat beta-glucan extract

A technology of extracting liquid and glucan, which is applied in the field of preparation of oat β-glucan extracting liquid, can solve the problems of not paying attention to conventional filtration problems such as pesticide residues, inconvenient practical application, high cost and unfavorable application, and achieve low cost , cost increase, strong application effect

Inactive Publication Date: 2017-06-13
上海芝映生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Patent CN201510115883.5 can better prepare oat β-glucan with different molecular weights, but the process is too complicated and the cost is too high to be conducive to application; Oat β-glucan, as oat β-glucan liquid for cosmetics, the existence of macromolecules will make the product unstable, and precipitation will occur during the shelf life. The membrane filtration technology adopted in CN200910199481.2 and CN201210064262. Loss of active ingredients, and because the previous process cannot remove fat, starch and other macromolecular substances, the membrane flux will drop rapidly, which is very inconvenient for practical application. In addition, the current patents do not pay attention to the problems of conventional filtration and pesticide residues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Oat bran was washed 4 times with 8 times of tap water, the residue was extracted by enzymatic hydrolysis with α-medium temperature amylase, the temperature was 55°C, the dosage was 10 U / g dry oat bran, enzymatic hydrolysis for 2 hours, pH was 4, and purified water was 4 times After the extraction, filter, freeze the filtrate at -20°C for 24 hours, and filter it while it is cold after dissolving. The filtrate is precipitated with ethanol with a concentration of 95%. After the alcohol precipitation, the solution reaches 30°, refrigerated overnight, and filtered. Activated carbon with supercritical CO 2 Impurity removal, operating pressure 25MPA, temperature 35°C, time 2h, flow rate 2000L / H, add 95 times purified water to the raffinate and stir at 95°C for 1.5h, filter and replenish water until the ratio of wheat bran and final extract is 1:50, that is Obtain oat β-glucan extract.

Embodiment 2

[0022] Example 2: oat bran was washed 3 times with 10 times of tap water, the filter residue was extracted by enzymatic hydrolysis with α-medium temperature amylase, the temperature was 50°C, the dosage was 20U / g dry oat bran, enzymatic hydrolysis for 2 hours, pH was 6, and purified water was 6 times After the extraction, filter the filtrate, freeze it at -5°C for 48 hours, and filter it while it is cold after dissolving. The filtrate is precipitated with ethanol with a concentration of 95%. After the alcohol precipitation, the solution reaches 40°, refrigerated overnight, and filtered. Activated carbon with supercritical CO 2 Impurity removal, operating pressure 20MPA, temperature 40°C, time 1h, flow rate 1500L / H, add 85 times purified water to the raffinate and stir at 95°C for 2h, filter and add water until the ratio of wheat bran and final extract is 1:50, that is, Oat beta-glucan extract.

Embodiment 3

[0023] Example 3: Oat bran was washed 4 times with 9 times of tap water, the filter residue was extracted by enzymatic hydrolysis with medium temperature amylase, the temperature was 53°C, the dosage was 15U / g of dry oat bran, the enzymatic hydrolysis was 2 hours, the pH was 5, and the amount of purified water was 5 times. After the extraction, filter, freeze the filtrate at -10°C for 36 hours, and filter it while it is cold after dissolving. The filtrate is precipitated with ethanol with a concentration of 95%. After the alcohol precipitation, the solution reaches 35°, refrigerated overnight, and filtered. with supercritical CO 2 Remove impurities, operating pressure 25MPA, temperature 35°C, time 2h, flow rate 2000L / H, add 95 times purified water to the raffinate and stir at 95°C for 2h, filter and replenish water until the ratio of wheat bran and final extract is 1:50, that is, Oat beta-glucan extract.

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Abstract

The invention discloses a preparation method for oat beta-glucan extract. The preparation method includes the steps of 1), washing and filtering; 2), enzymolysis and filtering; 3), freezing and thawing and filtering; 4), wine sinking and filtering; 5), supercritical CO2 removal; 6), redissolving. The preparation method is simple in process, low in cost, convenient in application, stable in product and free of pesticide residue.

Description

technical field [0001] The invention belongs to the technical field of cosmetic plant raw materials, and in particular relates to a preparation method of oat beta-glucan extract. Background technique [0002] Oat glucan is extracted from oat bran, and the added value of the product increases greatly after extraction. Oat glucan is a viscous polyglucan linked by β-1,3-, β-1,4-glycosidic bonds, which is mainly concentrated in the aleurone layer of oat bran. Oat glucan has multiple functions, such as lowering blood lipids, controlling blood sugar, enhancing immunity, preventing constipation, improving intestinal microenvironment, etc., and is widely used in the fields of medicine and health food. [0003] Patent CN201510115883.5 can better prepare oat β-glucan with different molecular weights, but the process is too complicated and the cost is too high to be conducive to application; Oat β-glucan, as oat β-glucan liquid for cosmetics, the existence of macromolecules will make...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
CPCC08B37/0003C08B37/0024Y02P20/54
Inventor 陈増焰
Owner 上海芝映生物科技有限公司
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