Preparation method and applications of electrochemical luminescence immunosensor sensing interface
A technology of sensing interface and luminescence immunity, which is applied in the fields of electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problems of complex electrode modification steps and poor reproducibility, and achieve the effect of avoiding deactivation problems and wide application prospects.
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Embodiment 1
[0020] Example 1 (1) 100 µL of 10 mg·mL -1 Graphene oxide aqueous solution, 100 µL 10mg·mL -1 The aqueous solution of carbon nitride nanosheets and 200 µL have a mass fraction of 30 mg·mL -1 The aqueous solution of tannic acid was mixed, ultrasonically oscillated for 5 minutes, and the concentration was 1 mol L with 50 µL -1 The sodium hydroxide solution adjusts the pH to 7;
[0021] (2) Mix the above mixed solution with 300 µL of an aqueous solution of bis(2-hydroxypropionic acid)diammonium dihydroxide titanium with a mass fraction of 40%, and vortex for 1 minute to obtain a sol doped with nanomaterials;
[0022] (3) Drop 10 µL of the sol prepared in step (2) on the surface of the treated and activated glassy carbon electrode with a diameter of 4 mm, and obtain a conductive gel-modified glassy carbon electrode after 30 minutes;
[0023] (4) Dispense 5 µL of prostate-specific antigen capture antibody solution (10 µg·mL) with a pipette gun -1 ) drop-coated on the surface of...
Embodiment 2
[0026] Example 2 (1) 100 µL of 10 mg·mL -1 Carbon nanotube aqueous solution, 100 µL 10mg·mL -1 The aqueous solution of carbon nitride nanosheets and 200 µL have a mass fraction of 30 mg·mL -1 The aqueous solution of tannic acid was mixed, ultrasonically oscillated for 5 minutes, and the concentration was 1 mol L with 50 µL -1 The sodium hydroxide solution adjusts the pH to 7;
[0027] (2) Mix the above mixed solution with 300 µL of an aqueous solution of bis(2-hydroxypropionic acid)diammonium dihydroxide titanium with a mass fraction of 40%, and vortex for 1 minute to obtain a sol doped with nanomaterials;
[0028] (3) Drop 10 µL of the sol prepared in step (2) on the surface of the treated and activated glassy carbon electrode with a diameter of 4 mm, and obtain a conductive gel-modified glassy carbon electrode after 30 minutes;
[0029] (4) Use a pipette gun to transfer 5 µL of breast cancer susceptibility gene capture antibody solution (10 µg·mL -1 ) drop-coated on the ...
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