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Primers for detecting polymorphism of PACSIN2 rs2413739 gene and PCR (polymerase chain reaction) method thereof

A gene polymorphism and primer pair technology, applied in the field of medical biology, can solve the problem of high incidence of adverse reactions, and achieve the effect of simple and easy method and wide application prospect.

Inactive Publication Date: 2017-06-20
SUN YAT SEN UNIV
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Problems solved by technology

However, this class of drugs has a high incidence of adverse reactions, including bone marrow suppression, liver damage, gastrointestinal disturbances, pancreatitis, and influenza-like symptoms

Method used

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  • Primers for detecting polymorphism of PACSIN2 rs2413739 gene and PCR (polymerase chain reaction) method thereof
  • Primers for detecting polymorphism of PACSIN2 rs2413739 gene and PCR (polymerase chain reaction) method thereof
  • Primers for detecting polymorphism of PACSIN2 rs2413739 gene and PCR (polymerase chain reaction) method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Primers for detecting PACSIN2 rs2413739 gene polymorphism and PCR method thereof

[0026] 1. Reagents and configuration required for the experiment

[0027] (1) DNA extraction reagent

[0028] 6mol / L NaI (Shanghai Shenggong Biological Engineering Technology Service Co., Ltd., Shanghai), chloroform, isopropanol, isoamyl alcohol, and ethanol are all commercially available analytical pure products.

[0029] (2) PCR amplification reagents

[0030] Ex Taq® enzyme (Takara company, Japan), synthetic primer (Shanghai Shenggong Bioengineering Technology Service Co., Ltd., Shanghai).

[0031] (3) Agarose gel electrophoresis reagent

[0032] Ordinary agarose (Biowest Company, Spain), low melting point agarose (Shanghai Bioengineering Technology Services Co., Ltd., Shanghai), 5×TBE electrophoresis solution storage solution (0.45 mol / L Tris base, 0.45 mol / L boric acid, 0.01 mol / L EDTA, adjust the pH to 8.0; Tris, boric acid, and EDTA were purchased from Shanghai Shenggong Biological...

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Abstract

The invention discloses a primer pair PrF / PrR for detecting a polymorphic site of a PACSIN2 rs2413739 gene by a PCR (polymerase chain reaction) method. Sequences are respectively shown in SEQ ID NO.1 and NO.2. The invention also discloses application of the primer pair PrF / PrR in detecting the polymorphic site of the PACSIN2 rs2413739 gene of a biological sample, and a kit prepared by the primer pair for detecting the polymorphic site of the PACSIN2 rs2413739 gene. The primer pair and the PCR method have the advantages that the gene type of the polymorphic site of the PACSIN2 rs2413739 gene can be determined according to the amplification and electrophoresis results, the therapy effect and side effect of a purinethol drug can be predicted, the validity and safety of a patient using the purinethol drug can be predicted on the basis of gene type analysis, the clinical drug application is instructed, and the individualized treatment of the patient can be performed.

Description

Technical field [0001] The invention belongs to the field of medical biotechnology. More specifically, it relates to a primer for detecting PACSIN2 rs2413739 gene polymorphism and a PCR method thereof. Background technique [0002] Inflammatory bowel disease (IBD) is a special chronic intestinal inflammatory disease, mainly including Crohn's disease (CD) and ulcerative colitis (UC). At present, in clinical practice, mercaptopurine drugs are often given to patients because they have a definite effect and are cheap. However, such drugs have a high incidence of adverse reactions, including bone marrow suppression, liver damage, gastrointestinal disorders, pancreatitis and influenza-like symptoms. [0003] Genetic background may not only affect the occurrence of diseases, but also affect individual differences in drug efficacy. Gene polymorphisms have an important impact on adverse drug reactions. Therefore, genetic screening methods are used to achieve individualized clinical medic...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156C12Q2600/106
Inventor 黄民王雪丁管少兴郑洪朱霞庄玮周珊胡品津高翔郅敏晁康
Owner SUN YAT SEN UNIV
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