A kind of near-infrared two-region fluorescent quantum dot probe and its preparation method and application
A fluorescent quantum dot and near-infrared technology, applied in the chemical field, can solve problems affecting the detection effect and achieve the effects of avoiding background noise, high sensitivity, and reducing surface defects
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Embodiment 1
[0052] A method for preparing a fluorescent quantum dot probe in the second near-infrared region, comprising the steps of:
[0053] (1) Preparation of silver sulfide quantum dots: 5mmol AgNO 3 Dissolve in 20ml of deionized water to form a colorless and transparent solution, then add concentrated ammonia water dropwise until the brown precipitate generated completely disappears, then transfer the resulting solution to the reaction kettle, add 3ml of n-dodecanethiol, and Place it in an oven at 200°C for 2 hours to obtain;
[0054] (2) Surface modification: After adding 25ml of ultrapure water and 5ml of mercaptoacetic acid to the reaction product obtained in step (1), place it in an ultrasonic generator for processing for 1h, wherein the frequency of the ultrasonic wave is 40kHz, and the power is 150W;
[0055] (3) Dialysis: filter the product obtained in step (2) through a filter with a pore size of 220 nm, and use a dialysis bag with a MWCO molecular weight cut-off of 100 DA ...
Embodiment 2
[0057] A method for detecting cadmium ion content using a fluorescent quantum dot probe in the second near-infrared region, wherein the fluorescent quantum dot probe in the second near-infrared region refers to the probe prepared in Example 1, and the method comprises the following steps:
[0058] (1) Prepare the silver sulfide quantum dot detection system: configure the Tris-HCl buffer solution with pH=7.4 according to the standard method, and configure the silver sulfide quantum dots with the volume ratio of the silver sulfide quantum dot stock solution and the Tris-HCl buffer solution at a volume ratio of 1:14 detection system.
[0059] (2) Preparation of standard solution: use Tris-HCl buffer solution with pH=7.4 to prepare concentrations of 1.5×10 -4 , 3×10 -4 , 4.5×10 -4 , 6×10 -4 , 7.5×10 -4 , 9×10 -4 , 10.5×10 -4 , 12×10 -4 The cadmium chloride solution of M, obtains the standard solution;
[0060] (3) Prepare the known solution to be tested: add 100 μL of the ...
experiment example 1
[0063] Experimental Example 1 Accuracy and Detection Limit Investigation
[0064] (1) Prepare the silver sulfide quantum dot detection system: configure the Tris-HCl buffer solution with pH=7.4 according to the standard method, and configure the silver sulfide quantum dots with the volume ratio of the silver sulfide quantum dot stock solution and the Tris-HCl buffer solution at a volume ratio of 1:14 detection system.
[0065] (2) Utilize the Tris-HCl buffer solution of pH=7.4 to prepare concentrations of 3×10 -3 , 3×10 -4 M in cadmium chloride solution. Add 100μL, 80μL, 60μL, 40μL, 30μL, 20μL to the quartz cuvette successively, the concentration is 3×10 -3 M, 100μL, 80μL, 60μL, 40μL, 20μL concentration is 3×10 -4 M cadmium chloride solution, and add silver sulfide quantum dot detection system to 3mL, with 3mL pure silver sulfide quantum dot detection system as the control group. Mix the two evenly and put them into an infrared fluorescence test system to detect their flu...
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