A comprehensive conditioner for promoting the degradation of mesophilic aerobic microorganisms and its preparation and use method

Abstract

The invention relates to the field of organic contaminated soil microbial remediation, in particular to a synthetical conditioner for promoting degradation of mesophilic aerobic microorganisms (petroleum pollutants) and a preparation and application method of the synthetical conditioner. A dry preparation is made of carbonized rice hulls with the dissolved organic carbon (DOC) content ratio being 2-4% (w / w). A wet preparation is an aqueous solution composed of one or more of KNO3, KH2PO4 and K2HPO4. Through application of the synthetical conditioner, the soil porosity can be increased, the air permeability is enhanced, and the storage capacity of carbon which is utilized by the microorganisms easily is improved; meanwhile, the synthetical conditioner is supplemented into soil in the mode that a carbon source acts quickly or is released slowly, and the ratio of C to N to P in the soil is optimized; and moreover, the contact performance of the microorganisms and petroleum hydrocarbon pollutants is improved through the adsorption effect of the surface of part of carbonized rice hulls, and efficient and continuous remediation of the mesophilic aerobic microorganisms is facilitated.

Description

Technical field [0001] The invention relates to the field of microbial remediation of organic contaminated soil, in particular to a comprehensive conditioner for promoting the degradation of mid-temperature aerobic microorganisms (petroleum pollutants) and a preparation and use method thereof. Background technique [0002] Microbial remediation technology as a cheap and environmentally friendly remediation technology has been widely used in the remediation process of petroleum contaminated soil. Aiming at the defects of low repair efficiency and slow repair rate of this technology, researches and attempts have been carried out on various regulation methods such as biological amplification and biological stimulation. The main factor restricting the efficiency of microbial remediation comes from the dynamic changes of soil physical and chemical properties. As the remediation process continues, soil nutrients are gradually lacking. Among them, the content of soil organic matter, es...

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Problems solved by technology

Among them, the content of soil organic matter, especially the soluble organic carbon (DOC) is constantly decreasing, and the ratio of C:N:P in the soil is out of balance with the consumption of carbon sources available to microorganisms, resulting in a gradual decline in the physiological state of microorganisms, a decrease in the abundance of microorganisms, and a decrease in cell The activity of internal and external metabolic enzymes is weakened, so the ability to metabolize and mineralize petroleum hydrocarbons is reduced, and the repair rate is slowed down

In addition, with the progress of microbial respiration and metabolism, the oxygen content in the soil pores gradually decreases, and the soil redox potential gradually decreases, resulting in insufficient electron acceptors required for the mineralization of petroleum hydrocarbons, and the microbial degradation ability is weakened

Furthermore, the adsorption of petroleum hydrocarbon molecules by soil is an important factor restricting its bioaccessibility and bioavailability, thus limiting the development of microbial metabolic capabilities

[0003] Therefore, for the above unfavorable factors, many adjustment countermeasures have been tried, including the addition of exogenous carbon sources, soil plowing and aeration, etc. The generation of metabolic competition hinders the remediation process, and the addition of a large amount of exogenous organic carbon in a short period of time is not conducive to the maintenance of microbial remediation efficiency for a long time, and the operation process such as soil plowing is tedious and time-consuming. These potential disadvantages Factors still restrict the further improvement of microbial remediation efficiency

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