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Application of puerarin hydrogel in drug for treating myocardial infarction

A technology of myocardial infarction and puerarin, applied in the field of puerarin small-molecule hydrogel, can solve problems such as insufficient retention, cell survival, and insufficient number of myocardial cells, and achieve the effect of promoting effect, promoting repair, and good anti-peroxidation effect

Inactive Publication Date: 2017-07-18
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Myocardial infarction is a serious threat to human health. None of the current treatments can solve the key problem of absolute shortage of myocardial cells after myocardial infarction. Stem cell transplantation therapy has the problem of insufficient cell survival and retention after transplantation

Method used

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  • Application of puerarin hydrogel in drug for treating myocardial infarction
  • Application of puerarin hydrogel in drug for treating myocardial infarction
  • Application of puerarin hydrogel in drug for treating myocardial infarction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the preparation of puerarin hydrogel

[0034] Weigh puerarin, add PBS (phosphate buffered saline), so that the concentration of puerarin in PBS is 2%wt, 4%wt, 6%wt, and heat it to 80°C-100°C with an alcohol lamp to completely dissolve the puerarin After naturally cooling to room temperature for 5 minutes, a jelly-like puerarin hydrogel can be formed.

Embodiment 2

[0035] Example 2: Effects of Puerarin Hydrogel on the Survival and Proliferation of Cardiomyocytes and Cardiac Fibroblasts

[0036] 1) Put puerarin hydrogels of different concentrations (2%, 4% and 6%) in a 96-well plate, add cells to the wells, and set the cell density to 5×10 4 / ml, no hydrogel was used as the control group, and different concentrations of puerarin hydrogels without cells were used as blank wells;

[0037] 2) When the measurement time comes, use a pipette to suck out the original medium, wash it twice with PBS, and add 10 μl of the solution in the CCK-8 kit to each well with a pipette gun;

[0038] 3) Place the 96-well plate in a 37°C, 5% carbon dioxide incubator and incubate for 4 hours;

[0039] 4) Take out the 96-well plate from the incubator, place it on a horizontal shaker, and shake it slowly for 10 minutes;

[0040] 5) Use a microplate reader to set the wavelength to 450 nm for absorbance measurement.

[0041] For experimental results, see figur...

Embodiment 3

[0042] Embodiment 3: Puerarin hydrogel to H 2 o 2 Influence of SOD activity and MDA content in induced MSCs cells

[0043] In this experiment, H 2 o 2 As an exogenous ROS mimicking the peroxidative environment after myocardial infarction in vivo, H 2 o 2 The concentration is 100μmol / L, intervene for 1h; experimental group: normal MSCs culture group, H 2 o 2 Intervention group, H 2 o 2 +2%PG (puerarin hydrogel) group, H 2 o 2+4% PG group, H 2 o 2 +6%PG group; intracellular superoxide dismutase (SOD) activity and intracellular malondialdehyde (MDA) content were detected according to the detection instructions.

[0044] For experimental results, see figure 2 , compared with the normal group, H 2 o 2 After induction for 1 h, the SOD activity and MDA content of MSCs were significantly increased (p2 o 2 The level of oxidative stress caused by H 2 o 2 Compared with two groups, 2%, 4% and 6% puerarin hydrogels could significantly reduce intracellular SOD activity...

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Abstract

The invention relates to an application of an early found self-assembled micro-molecular puerarin hydrogel in myocardium repairing after myocardial infarction. An in vitro experiment part according to the invention proves that the hydrogel has excellent cytocompatibility and anti-peroxidation capacity; an in vivo experiment part proves that the puerarin hydrogel can be used for repairing the myocardial infarction tissues in the manner of injection, can carry the mesenchymal stem cells and can promote the effect of repairing myocardial infarction through transplanted cells; a research result proves that the hydrogel has an excellent application prospect in myocardial infarction treatment.

Description

technical field [0001] The invention relates to the application of a small-molecule puerarin hydrogel formed by self-assembly in myocardial repair. Background technique [0002] Myocardial infarction is a serious threat to human health. None of the current treatment methods can solve the key problem of absolute shortage of myocardial cells after myocardial infarction. Stem cell transplantation treatment has the problem of insufficient cell survival and retention after transplantation. Injectable hydrogel preparations can provide physical support on the one hand, and can carry cells or drugs for transplantation after myocardial infarction on the other hand. Recent clinical trials have also confirmed the safety and effectiveness of injectable hydrogels in the treatment of myocardial infarction, showing good application prospects. In our previous work, we found that puerarin can self-assemble into hydrogel without any chemical modification, which is the first self-assembled hy...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K31/352A61K35/28A61L27/52A61L27/54A61L27/50A61L27/38A61P9/10A61P39/06A61P9/00
CPCA61K9/06A61K31/352A61K35/28A61L27/3834A61L27/50A61L27/52A61L27/54A61L2400/06A61K2300/00
Inventor 陈敏生杨志谋张建武区彩文
Owner SOUTHERN MEDICAL UNIVERSITY
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